Overview

  • Product name

    Anti-Sonic Hedgehog antibody [5H4]
    See all Sonic Hedgehog primary antibodies
  • Description

    Mouse monoclonal [5H4] to Sonic Hedgehog
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Human, African green monkey
  • Immunogen

    Purified Recombinant fragment, corresponding to amino acids 26-161 of Human Sonic Hedgehog expressed in E. Coli.

  • Positive control

    • Human Sonic Hedgehog recombinant protein; Sonic Hedgehog (amino acids 26-161)-hIgGFc transfected HEK293 cell lysate; LNCaP, HepG2, PANC1),HeLa, SK-N-SH, F9, NIH 3T3, and COS7 cell lysates; HeLa cells; Human liver cancer tissue.
  • General notes

    This product was changed from ascites to supernatant. Lot no’s high than GR164113-18 are from Tissue Culture Supernatant

Applications

Our Abpromise guarantee covers the use of ab135240 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/2000. Predicted molecular weight: 49 kDa.
IHC-P 1/200 - 1/1000.
Flow Cyt 1/200 - 1/400.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

Target

  • Function

    Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • Tissue specificity

    Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • Involvement in disease

    Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • Sequence similarities

    Belongs to the hedgehog family.
  • Post-translational
    modifications

    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • Cellular localization

    Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Information by UniProt
  • Database links

  • Alternative names

    • HHG 1 antibody
    • HHG-1 antibody
    • HHG1 antibody
    • HLP 3 antibody
    • HLP3 antibody
    • Holoprosencephaly 3 antibody
    • HPE 3 antibody
    • HPE3 antibody
    • MCOPCB5 antibody
    • shh antibody
    • SHH_HUMAN antibody
    • SMMC I antibody
    • SMMCI antibody
    • Sonic Hedgehog (Drosophila) homolog antibody
    • sonic hedgehog homolog (Drosophila) antibody
    • Sonic hedgehog homolog antibody
    • Sonic hedgehog protein antibody
    • Sonic hedgehog protein C-product antibody
    • TPT antibody
    • TPTPS antibody
    see all

Images

  • Immunohistochemical analysis of Paraffin-embedded Human liver cancer tissue labelling Sonic Hedgehog with ab135240 at 1/200 dilution followed by DAB staining.
  • Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution + Human Sonic Hedgehog recombinant protein

    Predicted band size: 49 kDa



    Expected MW is 41 kDa.
  • All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

    Lane 1 : HEK293 cell lysate
    Lane 2 : HEK293 cell lysate, transfected with Sonic Hedgehog (amino acids 26-161)-hIgGFc

    Predicted band size: 49 kDa

  • All lanes : Anti-Sonic Hedgehog antibody [5H4] (ab135240) at 1/500 dilution

    Lane 1 : LNCaP cell lysate
    Lane 2 : HepG2 cell lysate
    Lane 3 : PANC1 cell lysate
    Lane 4 : HeLa cell lysate
    Lane 5 : SK-N-SH
    cell lysate
    Lane 6 : F9 cell lysate
    Lane 7 : NIH 3T3 cell lysate
    Lane 8 : COS7 cell lysate

    Predicted band size: 49 kDa

  • Flow cytometric analysis of HeLa cells labelling Sonic Hedgehog with ab135240 at 1/200 dilution (green). Negative control (red).

References

This product has been referenced in:

  • Chen WY  et al. IL-33/ST2 axis mediates hyperplasia of intrarenal urothelium in obstructive renal injury. Exp Mol Med 50:36 (2018). WB ; Mouse . Read more (PubMed: 29674622) »
  • Song M  et al. Adipocyte-Derived Exosomes Carrying Sonic Hedgehog Mediate M1 Macrophage Polarization-Induced Insulin Resistance via Ptch and PI3K Pathways. Cell Physiol Biochem 48:1416-1432 (2018). Read more (PubMed: 30064125) »
See all 2 Publications for this product

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