Anti-Sonic Hedgehog antibody (ab19897)

Reviews (7)Q&A (3)References (17)
Sandwich ELISA - Anti-Sonic Hedgehog antibody (ab19897)
  • Western blot - Anti-Sonic Hedgehog antibody (ab19897)

Key features and details

  • Rabbit polyclonal to Sonic Hedgehog
  • Suitable for: Sandwich ELISA, WB
  • Reacts with: Mouse
  • Isotype: IgG

Overview

  • Product name

    Anti-Sonic Hedgehog antibody
    See all Sonic Hedgehog primary antibodies

  • Description

    Rabbit polyclonal to Sonic Hedgehog

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Sandwich ELISA, WBmore details
    Unsuitable for: IHC-Fr

  • Species reactivity

    Reacts with: Mouse
    Predicted to work with: Rat, Chicken, Cow, Human, Xenopus laevis, Zebrafish, a wide range of other species

  • Immunogen

    Synthetic peptide corresponding to Human Sonic Hedgehog aa 1-100 (N terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab21448)

  • Positive control

    • This antibody gave a positive signal in Mouse Recombinant Sonic Hedgehog protein.

  • General notes

    The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.

    If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As

Properties

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab19897 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 48 kDa).
Notes
Sandwich ELISA
Use a concentration of 0.5 µg/ml. For sandwich ELISA, use this antibody as Detection at 0.5 µg/ml with Mouse monoclonal [10H6] to Sonic Hedgehog (ab87382) as Capture.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 26 kDa (predicted molecular weight: 48 kDa).
  • Application notes
    Is unsuitable for IHC-Fr.

    • Target

    • Function

      Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.

    • Tissue specificity

      Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.

    • Involvement in disease

      Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
      Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
      Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
      Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.

    • Sequence similarities

      Belongs to the hedgehog family.

    • Post-translational
      modifications

      The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
      Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
      N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.

    • Cellular localization

      Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
    • Information by UniProt

    • Database links

      see all

    • Alternative names

      • HHG 1 antibody
      • HHG-1 antibody
      • HHG1 antibody
      • HLP 3 antibody
      • HLP3 antibody
      • Holoprosencephaly 3 antibody
      • HPE 3 antibody
      • HPE3 antibody
      • MCOPCB5 antibody
      • shh antibody
      • SHH_HUMAN antibody
      • SMMC I antibody
      • SMMCI antibody
      • Sonic Hedgehog (Drosophila) homolog antibody
      • sonic hedgehog homolog (Drosophila) antibody
      • Sonic hedgehog homolog antibody
      • Sonic hedgehog protein antibody
      • Sonic hedgehog protein C-product antibody
      • TPT antibody
      • TPTPS antibody
      see all

    Images

    References (17)

    Publishing research using ab19897? Please let us know so that we can cite the reference in this datasheet.

    ab19897 has been referenced in 17 publications.

    • Zhang Y  et al. Role of VIP and Sonic Hedgehog Signaling Pathways in Mediating Epithelial Wound Healing, Sensory Nerve Regeneration, and Their Defects in Diabetic Corneas. Diabetes 69:1549-1561 (2020). PubMed: 32345752
    • Ren Y  et al. Regulation of miR-375 and Sonic hedgehog on vascular endothelial growth factor in preeclampsia rats and its effect on trophoblast cells. Biosci Rep N/A:N/A (2020). PubMed: 32412057
    • Tarulli GA  et al. Discrete Hedgehog Factor Expression and Action in the Developing Phallus. Int J Mol Sci 21:N/A (2020). PubMed: 32059607
    • Sun Q  et al. microRNA-9 and -29a regulate the progression of diabetic peripheral neuropathy via ISL1-mediated sonic hedgehog signaling pathway. Aging (Albany NY) 12:11446-11465 (2020). PubMed: 32544883
    • Chen J  et al. The anti-inflammation, anti-oxidative and anti-fibrosis properties of swertiamarin in cigarette smoke exposure-induced prostate dysfunction in rats. Aging (Albany NY) 11:10409-10421 (2019). PubMed: 31739286
    View all Publications for this product

    Customer reviews and Q&As

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    1-3 of 3 Q&A

    Question

    sELISA is still not working. Would like ELISA kit ab100639 as a replacement.

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    Answer

    Thank you for your call today, and I apologize that these antibodies still are not working.

    As we discussed, I'm sending a free of charge ELISA kit ab100639 on the order ***, which I expect to arrive by Friday.

    Please keep me updated about how this kit works for you, and let me know if you have any questions or if there is anything else that we can do for you.

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    Question

    No signal response in sELISA, usinghuman recombinant protein as antigen.

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    Answer

    Thank you for your call last week and for your patience while I have been in touch with my colleagues about these antibodies. I apologize for the delay getting back to you.

    The capture antibody ab87382 was tested at a coating concentration of 1 ug/mL, which corresponds to a 1:200 dilution as the antibody is provided at 0.2 mg/mL. If the antibody was used at 1:1000, this is a 0.2 ug/mL dilution which might have contributed to a weak signal in the ELISA. I would recommend decreasing the concentration of this antibody to 1:200. The detection antibody ab19897 was tested at 0.5 ug/mL, which is approximately the same concentration that you have used (the antibody is provided at 0.6 mg/mL for the lot that you have).

    We had also discussed testing the secondary antibody to see if it cross-reacts with the mouse capture antibody ab87382. Have you had a chance to test this?

    We do guarantee these antibodies to work in your assay, so if you think that they are faulty, I would be happy to send a replacement or issue a credit or refund if you prefer. Please let me know if you have any questions, and keep me updated about any progress with these antibodies. Feel free to call 888-772-2226 and ask to speak to me anytime.

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    Question

    Customer interested in testing ab19897 in Zebrafish

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    Answer

    DISCOUNT CODE: XXXXXXXX
    Expiration date: 18-05-2012

    I am very pleased to hear you would like to accept our offer and test ab19897 in ZebraFish. This code will give you 1 free [PRIMARY ANTIBODY/PROTEIN] before the expiration date. To redeem this offer, please submit an Abreview forZebraFish and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews.


    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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