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  1. Link

    sonic-hedgehog-antibody-ep1190y-ab53281.pdf

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RecombinantRabMAb

Recombinant Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

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Reviews (6)Q&A (9)References (69)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
  • Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EP1190Y] to Sonic Hedgehog
  • Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-P
  • Reacts with: Human

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Overview

  • Product name

    Anti-Sonic Hedgehog antibody [EP1190Y]
    See all Sonic Hedgehog primary antibodies
  • Description

    Rabbit monoclonal [EP1190Y] to Sonic Hedgehog
  • Host species

    Rabbit
  • Specificity

    Specific for both full length (51kDa) and c-product subunit (27kDa) of human Sonic Hedgehog protein
  • Tested applications

    Suitable for: Flow Cyt (Intra), ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human Sonic Hedgehog. The exact sequence is proprietary.
    (Peptide available as ab203144)

  • Positive control

    • Human fetal liver and kidney lysates, human kidney, fetal membrane and human pancreatic carcinoma tissue and HeLa cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or outlicensing@thermofisher.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Avoid freeze / thaw cycle. Stable for 12 months at -20°C.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 59% PBS, 40% Glycerol, 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EP1190Y
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Embryonic Stem Cells
    • Surface Molecules
    • Stem Cells
    • Neural Stem Cells
    • Surface Molecules
    • Stem Cells
    • Signaling Pathways
    • Hedgehog
    • Secreted
    • Cancer
    • Oncoproteins/suppressors
    • Tumor suppressors
    • PTC & Wnt pathway
    • Developmental Biology
    • Embryogenesis
    • Morphogens
    • Developmental Biology
    • Embryogenesis
    • Embryonic stem cells
    • Intracellular
    • Developmental Biology
    • Organogenesis
    • Gut development
    • Gut specification
    • Cancer
    • Cancer Metabolism
    • Response to hypoxia
    • Metabolism
    • Pathways and Processes
    • Metabolism processes
    • Hypoxia
    • Metabolism
    • Types of disease
    • Cancer

Associated products

  • Alternative Versions

    • Anti-Sonic Hedgehog antibody [EP1190Y] - BSA and Azide free (ab175180)
    • Alexa Fluor® 488 Anti-Sonic Hedgehog antibody [EP1190Y] (ab203961)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
    • Goat Anti-Rabbit IgG H&L (DyLight® 488) preadsorbed (ab96899)
  • Immunizing Peptide (Blocking)

    • Sonic Hedgehog peptide (ab203144)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab150035)
  • Recombinant Protein

    • Recombinant human Sonic Hedgehog protein (ab123773)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab53281 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF (1)
1/250 - 1/500.
WB (2)
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Can be blocked with Sonic Hedgehog peptide (ab203144).
IHC-P (3)
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.

Notes
Flow Cyt (Intra)
1/10 - 1/100.

ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.

ICC/IF
1/250 - 1/500.
WB
1/1000 - 1/10000. Detects a band of approximately 51 kDa (predicted molecular weight: 51 kDa).Can be blocked with Sonic Hedgehog peptide (ab203144).
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

See IHC antigen retrieval protocols.

For unpurified use at 1/100.

Target

  • Function

    Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction.
  • Tissue specificity

    Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues.
  • Involvement in disease

    Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
    Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
    Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
    Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression.
  • Sequence similarities

    Belongs to the hedgehog family.
  • Post-translational
    modifications

    The C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
    Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
    N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity.
  • Cellular localization

    Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell.
  • Target information above from: UniProt accession Q15465 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6469 Human
    • Omim: 600725 Human
    • SwissProt: Q15465 Human
    • Unigene: 164537 Human
    • Alternative names

      • HHG 1 antibody
      • HHG-1 antibody
      • HHG1 antibody
      • HLP 3 antibody
      • HLP3 antibody
      • Holoprosencephaly 3 antibody
      • HPE 3 antibody
      • HPE3 antibody
      • MCOPCB5 antibody
      • shh antibody
      • SHH_HUMAN antibody
      • SMMC I antibody
      • SMMCI antibody
      • Sonic Hedgehog (Drosophila) homolog antibody
      • sonic hedgehog homolog (Drosophila) antibody
      • Sonic hedgehog homolog antibody
      • Sonic hedgehog protein antibody
      • Sonic hedgehog protein C-product antibody
      • TPT antibody
      • TPTPS antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human pancreatic carcinoma tissue sections labeling Sonic Hedgehog  with purified ab53281 at 1/2000 dilution (0.097 μg/ml). Heat mediated antigen retrieval was performed using EDTA Buffer, PH9. Tissue was counterstained with Hematoxylin. ab97051 Goat Anti-Rabbit IgG H&L (HRP) secondary antibody was used at 1/500 dilution. PBS instead of the primary antibody was used as the negative control.

    • Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Immunocytochemistry/ Immunofluorescence analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Sonic Hedgehog with purified  ab53281 at 1/250 dilution (0.8μg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889, Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1/200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor® 488) was used as the secondary antibody at 1/1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281) at 1/2000 dilution (purified) + Human fetal liver lysate at 15 µg

      Secondary
      Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution

      Predicted band size: 51 kDa
      Observed band size: 27,50 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM/TBST.

      50kDa: Full-length SHH; 27KDa: C-terminal product

    • Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Intracellular Flow Cytometry analysis of HeLa (Human epithelial cell line from cervix adenocarcinoma) cells labeling Sonic Hedgehog with purified ab53281 at 1/20 dilution (10 ug/ml) (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluorr® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - cells without incubation with primary antibody and secondary antibody (Blue). 

       

       

    • Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Immunocytochemistry/Immunofluorescence analysis of HeLa (human cervix adenocarcinoma) labelling Sonic Hedgehog with purified ab53281 at 1/500. Cells were fixed with 4% Paraformaldehyde and permeabilised by 0.1% tritonX-100. An Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody (Ab150077). Nuclei counterstained with DAPI (blue).

    • Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Immunocytochemistry/ Immunofluorescence - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Immunocytochemistry/Immunofluorescence analysis of HepG2 cells labelling Sonic Hedgehog with unpurified ab53281.

    • Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281) at 1/1000 dilution (purified) + Human fetal kidney lysate at 15 µg

      Secondary
      Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 27 kDa why is the actual band size different from the predicted?



      Blocking and diluting buffer: 5% NFDM /TBST .

      27KDa: C-terminal product.

    • Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Intracellular Flow Cytometry analysis of HeLa (human cervix adenocarcinoma) cells labelling Sonic Hedgehog with unpurified ab53281 at 1/20 (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. An Alexa Fluorr®488-conjugated goat anti-rabbit IgG (1/2000) was used as the secondary antibody. Black - Isotype control, rabbit monoclonal IgG. Blue - Unlabelled control, cells without incubation with primary and secondary antibodies. 

       

       

    • Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Western blot - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281) at 1/2000 dilution (unpurified) + Fetal liver membrane at 10 µg

      Secondary
      Goat anti-rabbit HRP at 1/2000 dilution

      Predicted band size: 51 kDa
      Observed band size: 51 kDa
      Additional bands at: 27 kDa (possible isoform), 49 kDa. We are unsure as to the identity of these extra bands.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Immunohistochemical analysis of Formalin fixed paraffin-embedded human kidney cancerous tissue labeling Sonic Hedgehog with unpurified ab53281 at 1/100 dilution.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)Image from McCann CK et al., PLoS One. 2011;6(11):e28077. Epub 2011 Nov 29. Fig 1.; doi:10.1371/journal.pone.0028077; November 29, 2011, PLoS ONE 6(11): e28077.

      Immunohistochemical analysis of Formalin fixed paraffin embedded human ovarian cancer tissue, staining Sonic Hedgehog with unpurified ab53281.

      Antigen retrieval was carried out in citrate buffer using a pressure cooker for 40 minutes. Sections were blocked with blocking agent before incubating with primary antibody (1/2000) for 90 minutes at room temperature. Staining was detected using DAB.

    • Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Flow Cytometry (Intracellular) - Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

      Overlay histogram showing HepG2 cells stained with unpurified ab53281 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53281, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in HepG2 cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions. 

       

       

    • Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)
      Anti-Sonic Hedgehog antibody [EP1190Y] (ab53281)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (69)

    Publishing research using ab53281? Please let us know so that we can cite the reference in this datasheet.

    ab53281 has been referenced in 69 publications.

    • Pan Y  et al. Dysfunction of Shh signaling activates autophagy to inhibit trophoblast motility in recurrent miscarriage. Exp Mol Med 53:52-66 (2021). PubMed: 33390589
    • Peng Y  et al. Dual activation of Hedgehog and Wnt/ß-catenin signaling pathway caused by downregulation of SUFU targeted by miRNA-150 in human gastric cancer. Aging (Albany NY) 13:10749-10769 (2021). PubMed: 33848981
    • Liu T  et al. MicroRNA-26a inhibits cell proliferation and invasion by targeting FAM98A in breast cancer. Oncol Lett 21:367 (2021). PubMed: 33747224
    • Zhu Z  et al. NAT10 Promotes Osteogenic Differentiation of Mesenchymal Stem Cells by Mediating N4-Acetylcytidine Modification of Gremlin 1. Stem Cells Int 2021:8833527 (2021). PubMed: 33953754
    • Tang N  et al. Possible involvement of the Hedgehog and PDPK1-Akt pathways in the growth and migration of small-cell lung cancer. J Int Med Res 49:3000605211016562 (2021). PubMed: 34038205
    View all Publications for this product

    Customer reviews and Q&As

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    1-6 of 6 Abreviews

    Immunocytochemistry/ Immunofluorescence abreview for Anti-Sonic Hedgehog antibody [EP1190Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Human Cell (Human Kidney, FFPE cortex/medulla)
    Permeabilization
    No
    Specification
    Human Kidney, FFPE cortex/medulla
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Lindsey Fitzsimons

    Verified customer

    Submitted Oct 28 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sonic Hedgehog antibody [EP1190Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (tooth)
    Antigen retrieval step
    None
    Permeabilization
    No
    Specification
    tooth
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 19 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sonic Hedgehog antibody [EP1190Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (pancreatic tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization
    No
    Specification
    pancreatic tumor
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 14 2015

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-Sonic Hedgehog antibody [EP1190Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Mouse Tissue sections (Pancreatic nodule)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: 10mM Citrate buffer pH 6
    Permeabilization
    No
    Specification
    Pancreatic nodule
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Fixative
    10% buffered Formalin
    Read More

    Abcam user community

    Verified customer

    Submitted Aug 07 2015

    Western blot abreview for Anti-Sonic Hedgehog antibody [EP1190Y]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Loading amount
    50 µg
    Gel Running Conditions
    Reduced Denaturing
    Sample
    Human Cell lysate - whole cell (fibroblasts)
    Specification
    fibroblasts
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
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    Abcam user community

    Verified customer

    Submitted Mar 27 2015

    Western blot abreview for Anti-Sonic Hedgehog antibody [EP1190Y]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - whole cell (Glioblastoma primary cells)
    Loading amount
    30 µg
    Specification
    Glioblastoma primary cells
    Gel Running Conditions
    Reduced Denaturing (10%)
    Blocking step
    Milk as blocking agent for 30 minute(s) · Concentration: 10% · Temperature: 20°C
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 21 2012

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