Anti-Sonic Hedgehog antibody [RM0128-4A37] (ab86462)
Key features and details
- Rat monoclonal [RM0128-4A37] to Sonic Hedgehog
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG2
Overview
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Product name
Anti-Sonic Hedgehog antibody [RM0128-4A37]
See all Sonic Hedgehog primary antibodies -
Description
Rat monoclonal [RM0128-4A37] to Sonic Hedgehog -
Host species
Rat -
Specificity
ab86462 detects Sonic Hedgehog, but not dhh or ihh. -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse -
Immunogen
Recombinant fragment from the C terminal region of mouse Sonic Hedgehog
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General notes
ab86462 was produced from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat).
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Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Constituent: PBS -
Concentration information loading...
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Purity
Immunogen affinity purified -
Purification notes
IgG fraction of culture supernatant purified by Protein A/G affinity chromatography and 0.2 µm filtered. -
Primary antibody notes
ab86462 was produced from a hybridoma (mouse myeloma fused with spleen cells from an immunized rat). -
Clonality
Monoclonal -
Clone number
RM0128-4A37 -
Isotype
IgG2 -
Research areas
Associated products
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Compatible Secondaries
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab86462 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P | (1) |
Use at an assay dependent concentration.
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WB |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. |
Target
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Function
Binds to the patched (PTC) receptor, which functions in association with smoothened (SMO), to activate the transcription of target genes. In the absence of SHH, PTC represses the constitutive signaling activity of SMO. Also regulates another target, the gli oncogene. Intercellular signal essential for a variety of patterning events during development: signal produced by the notochord that induces ventral cell fate in the neural tube and somites, and the polarizing signal for patterning of the anterior-posterior axis of the developing limb bud. Displays both floor plate- and motor neuron-inducing activity. The threshold concentration of N-product required for motor neuron induction is 5-fold lower than that required for floor plate induction. -
Tissue specificity
Expressed in fetal intestine, liver, lung, and kidney. Not expressed in adult tissues. -
Involvement in disease
Defects in SHH are the cause of microphthalmia isolated with coloboma type 5 (MCOPCB5) [MIM:611638]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, cataract and other abnormalities like cataract may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure).
Defects in SHH are the cause of holoprosencephaly type 3 (HPE3) [MIM:142945]. Holoprosencephaly (HPE) [MIM:236100] is the most common structural anomaly of the brain, in which the developing forebrain fails to correctly separate into right and left hemispheres. Holoprosencephaly is genetically heterogeneous and associated with several distinct facies and phenotypic variability. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. Interestingly, up to 30% of obligate carriers of HPE3 gene in autosomal dominant pedigrees are clinically unaffected.
Defects in SHH are a cause of solitary median maxillary central incisor (SMMCI) [MIM:147250]. SMMCI is a rare dental anomaly characterized by the congenital absence of one maxillary central incisor.
Defects in SHH are the cause of triphalangeal thumb-polysyndactyly syndrome (TPTPS) [MIM:174500]. TPTPS is an autosomal dominant syndrome characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. TPTPS mutations have been mapped to the 7q36 locus in the LMBR1 gene which contains in its intron 5 a long-range cis-regulatory element of SHH expression. -
Sequence similarities
Belongs to the hedgehog family. -
Post-translational
modificationsThe C-terminal domain displays an autoproteolysis activity and a cholesterol transferase activity. Both activities result in the cleavage of the full-length protein and covalent attachment of a cholesterol moiety to the C-terminal of the newly generated N-terminal fragment (N-product). The N-product is the active species in both local and long-range signaling, whereas the C-product has no signaling activity.
Cholesterylation is required for N-product targeting to lipid rafts and multimerization.
N-palmitoylation of Cys-24 by HHAT is required for N-product multimerization and full activity. -
Cellular localization
Cell membrane. The N-product either remains associated with lipid rafts at the cell surface, or forms freely diffusible active multimers with its hydrophobic lipid-modified N- and C-termini buried inside and Secreted > extracellular space. The C-terminal peptide diffuses from the cell. - Information by UniProt
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Database links
- Entrez Gene: 20423 Mouse
- SwissProt: Q62226 Mouse
- Unigene: 57202 Mouse
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Alternative names
- HHG 1 antibody
- HHG-1 antibody
- HHG1 antibody
see all
Datasheets and documents
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Datasheet download
References (2)
ab86462 has been referenced in 2 publications.
- Williamson I et al. Developmentally regulated Shh expression is robust to TAD perturbations. Development 146:N/A (2019). PubMed: 31511252
- Lettice LA et al. Development of five digits is controlled by a bipartite long-range cis-regulator. Development 141:1715-25 (2014). IHC-P ; Mouse . PubMed: 24715461