Product nameSophiaGreen™ 421
DescriptionMembrane impermeant DNA and chromatin green counterstain
Membrane impermeant DNA and chromatin green counterstain. Can be excited using excitation filters appropriate for green-fluorescent dyes. Does not stain live cells.
A unit contains 50 μl of a 1 mM solution in DMSO. Suggested starting concentration for dead cell determination is 50nM.
Storage instructionsShipped at 4°C. Store at -20°C. Store In the Dark. Store under desiccating conditions.
Soluble in DMSO
Refer to SDS for further information
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab275924 has been referenced in 3 publications.
- Fürstenberg A et al. Ultrafast excited-state dynamics of DNA fluorescent intercalators: new insight into the fluorescence enhancement mechanism. J Am Chem Soc 128:7661-9 (2006). PubMed: 16756323
- Rye HS & Glazer AN Interaction of dimeric intercalating dyes with single-stranded DNA. Nucleic Acids Res 23:1215-22 (1995). PubMed: 7739900
- Rye HS et al. Fluorometric assay using dimeric dyes for double- and single-stranded DNA and RNA with picogram sensitivity. Anal Biochem 208:144-50 (1993). PubMed: 7679561