Recombinant Anti-Sorbitol Dehydrogenase antibody [EPR15857] (ab189248)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR15857] to Sorbitol Dehydrogenase
- Suitable for: IP, WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-Sorbitol Dehydrogenase antibody [EPR15857]
See all Sorbitol Dehydrogenase primary antibodies -
Description
Rabbit monoclonal [EPR15857] to Sorbitol Dehydrogenase -
Host species
Rabbit -
Tested applications
Suitable for: IP, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal kidney, human fetal liver, mouse kidney, mouse spleen, rat kidney, C6, Hela, PC-3 cell lysates,
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR15857 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab189248 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IP |
1/50.
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WB |
1/1000 - 1/20000. Predicted molecular weight: 38 kDa.
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Notes |
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IP
1/50. |
WB
1/1000 - 1/20000. Predicted molecular weight: 38 kDa. |
Target
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Function
Converts sorbitol to fructose. Part of the polyol pathway that plays an important role in sperm physiology. May play a role in the sperm motility by providing an energetic source for sperm. -
Tissue specificity
Expressed in kidney and epithelial cells of both benign and malignant prostate tissue. Expressed in epididymis (at protein level). -
Sequence similarities
Belongs to the zinc-containing alcohol dehydrogenase family. -
Cellular localization
Mitochondrion membrane. Cell projection > cilium > flagellum. Associated with mitochondria of the midpiece and near the plasma membrane in the principal piece of the flagellum. Also found in the epididymosome, secreted by the epididymal epithelium and that transfers proteins from the epididymal fluid to the sperm surface. - Information by UniProt
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Database links
- Entrez Gene: 6652 Human
- Entrez Gene: 20322 Mouse
- Entrez Gene: 24788 Rat
- Omim: 182500 Human
- SwissProt: Q00796 Human
- SwissProt: Q64442 Mouse
- SwissProt: P27867 Rat
- Unigene: 878 Human
see all -
Alternative names
- DHSO_HUMAN antibody
- L iditol 2 dehydrogenase antibody
- L-iditol 2-dehydrogenase antibody
see all
Images
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All lanes : Anti-Sorbitol Dehydrogenase antibody [EPR15857] (ab189248) at 1/20000 dilution
Lane 1 : Human fetal kidney tissue lysate
Lane 2 : Human fetal liver tissue lysate
Lane 3 : Hela cell lysate
Lane 4 : PC-3 cell lysate
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
All lanes : Anti-Sorbitol Dehydrogenase antibody [EPR15857] (ab189248) at 1/5000 dilution
Lane 1 : Mouse kidney tissue lysate
Lane 2 : Mouse spleen tissue lysate
Lane 3 : Rat kidney tissue lysate
Lane 4 : C6 lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size: 38 kDa -
Immunoprecipitation of Human fetal liver labeling Sorbitol Dehydrogenase with ab189248 at 1/50 dilution and Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (2)
ab189248 has been referenced in 2 publications.
- Dong HL et al. Biallelic SORD pathogenic variants cause Chinese patients with distal hereditary motor neuropathy. NPJ Genom Med 6:1 (2021). PubMed: 33397963
- Cortese A et al. Biallelic mutations in SORD cause a common and potentially treatable hereditary neuropathy with implications for diabetes. Nat Genet 52:473-481 (2020). PubMed: 32367058