Key features and details
- Goat polyclonal to SORBS1
- Suitable for: WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SORBS1 antibody
See all SORBS1 primary antibodies
DescriptionGoat polyclonal to SORBS1
SpecificityThis antibody is expected to recognise both human isoforms.
Tested applicationsSuitable for: WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
General notesGenBank Accession Number – NP_006425; NP_056200. LocusLink ID - 10580 (human); 20411 (mouse). Gene Ontology terms - cytoskeletal protein binding; actin binding; cell-matrix adhesion; zonula adherens; cell-cell adherens junction.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage bufferPreservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab4551 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use at an assay dependent concentration.
A 1 hour primary incubation is recommended for this product. No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.
|IHC-P||Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
FunctionPlays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
Tissue specificityDetected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.
Sequence similaritiesContains 3 SH3 domains.
Contains 1 SoHo domain.
Cellular localizationCell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
- Information by UniProt
- c-Cbl-associated protein antibody
- CAP antibody
- CBL Associated Protein antibody
All lanes : Anti-SORBS1 antibody (ab4551)
Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)
All lanes : Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution
ab4551 was tested in in human plasma, human white blood cells and HEK293 cells. Samples were run for 1h at 200 V using the Fisherbrand vertical complete gel unit, and transferred onto nitrocellulose 0.45um (1h, 100V, 4 C) using the Bio-rad Trans blot cell with plate electrodes. Membranes were blocked for 1 h at RT, then incubated overnight in primary antibody (1/200, 1/1000, 1/5000 or minus primary antibody) at 4 degrees C, with shaking. Following washing, membranes were incubated in secondary antibody (1/5000, Alexa-Fluor680 donkey anti-goat IgG, H+L) for 1 h at RT. Antibody binding was detected using LI-COR Bioscience's Odyssey Infrared Imaging system (intensity 5). The molecular weight of sorbs 1 was calculated using 'Compute pI/MW'from ExPASy tools based on the amino acid sequence according to NP_006425. The band indicated is approximately this predicted molecular weight, and is not present in the minus primary image, scanned at the same intensity.
Image courtesy of
ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P. Steamed antigen retrieval in citrate buffer pH6, AP-staining. This image shows spotty staining on muscle fibres in longitudinal section.
ab4551 has been referenced in 2 publications.
- Holle AW et al. High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation. Integr Biol (Camb) 8:1049-1058 (2016). PubMed: 27723854
- Hallock PT et al. Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation. Mol Cell Biol 36:262-70 (2015). IF . PubMed: 26527617