• Product name

  • Description

    Goat polyclonal to SORBS1
  • Host species

  • Specificity

    This antibody is expected to recognise both human isoforms.
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide: GTFPGNYVKPLYL, corresponding to C terminal amino acids 804-816 of Human SORBS1.

  • General notes

    GenBank Accession Number – NP_006425; NP_056200. LocusLink ID - 10580 (human); 20411 (mouse). Gene Ontology terms - cytoskeletal protein binding; actin binding; cell-matrix adhesion; zonula adherens; cell-cell adherens junction.



Our Abpromise guarantee covers the use of ab4551 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent dilution. Can be blocked with Human SORBS1 peptide (ab18388). No signal obtained yet but low background observed in human heart and 293 lysates at up to 2 µg/ml.
IHC-P Use a concentration of 2.5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function

    Plays a role in tyrosine phosphorylation of CBL by linking CBL to the insulin receptor. Required for insulin-stimulated glucose transport. Involved in formation of actin stress fibers and focal adhesions.
  • Tissue specificity

    Detected in skeletal muscle (at protein level). Widely expressed with highest levels in heart and skeletal muscle.
  • Sequence similarities

    Contains 3 SH3 domains.
    Contains 1 SoHo domain.
  • Post-translational

  • Cellular localization

    Cell junction > adherens junction. Cell membrane. Cytoplasm > cytoskeleton. Cell junction > focal adhesion. Colocalizes with actin stress fibers. Also detected at the plasma membrane and in neuronal intranuclear inclusions. Colocalized with PXN at focal adhesions during myogenic differentiation.
  • Information by UniProt
  • Database links

  • Alternative names

    • c-Cbl-associated protein antibody
    • CAP antibody
    • CBL Associated Protein antibody
    • Ponsin antibody
    • SH3 domain protein 5 antibody
    • SH3D5 antibody
    • SH3P12 antibody
    • Sorbin and SH3 Domain Containing 1 antibody
    • Sorbin and SH3 domain-containing protein 1 antibody
    • Sorbs1 antibody
    • SRBS1_HUMAN antibody
    see all


  • All lanes : Anti-SORBS1 antibody (ab4551)

    Lane 1 : Molecular weight marker (Bio-Rad Precision Plus Kaleidoscope standards)
    Lane 2 : 10 µl Human plasma 1 (600-800 µg protein)
    Lane 3 : 10 µl Human plasma 2 (600-800 µg protein)
    Lane 4 : 10 µl Human White Blood cells 1 (30-50 µg protein)
    Lane 5 : 10 µl Human White Blood cells 2 (30-50 µg protein)
    Lane 6 : 10 µl HEK293 whole cell lysate (prepared by scrapping 10 cm confluent dish into 4ml of 2x sample buffer (Sigma)

    All lanes : Donkey anti-goat IgG (H+L) conjugated to Alexa-Fluor680 at 1/5000 dilution

    ab4551 was tested in in human plasma, human white blood cells and HEK293 cells. Samples were run for 1h at 200 V using the Fisherbrand vertical complete gel unit, and transferred onto nitrocellulose 0.45um (1h, 100V, 4 C) using the Bio-rad Trans blot cell with plate electrodes. Membranes were blocked for 1 h at RT, then incubated overnight in primary antibody (1/200, 1/1000, 1/5000 or minus primary antibody) at 4 degrees C, with shaking. Following washing, membranes were incubated in secondary antibody (1/5000, Alexa-Fluor680 donkey anti-goat IgG, H+L) for 1 h at RT. Antibody binding was detected using LI-COR Bioscience's Odyssey Infrared Imaging system (intensity 5). The molecular weight of sorbs 1 was calculated using 'Compute pI/MW'from ExPASy tools based on the amino acid sequence according to NP_006425. The band indicated is approximately this predicted molecular weight, and is not present in the minus primary image, scanned at the same intensity.

    Image courtesy of

  • ab4551 (2.5 µg/ml) staining SORBS1 in Human Skeletal Muscle by IHC-P.  Steamed antigen retrieval in citrate buffer pH6, AP-staining.  This image shows spotty staining on muscle fibres in longitudinal section.


This product has been referenced in:

  • Holle AW  et al. High content image analysis of focal adhesion-dependent mechanosensitive stem cell differentiation. Integr Biol (Camb) 8:1049-1058 (2016). Read more (PubMed: 27723854) »
  • Hallock PT  et al. Sorbs1 and -2 Interact with CrkL and Are Required for Acetylcholine Receptor Cluster Formation. Mol Cell Biol 36:262-70 (2015). IF . Read more (PubMed: 26527617) »
See all 2 Publications for this product

Customer reviews and Q&As

Western blot
Human Cell lysate - whole cell (white blood cells)
Loading amount
40 µg
white blood cells
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2% · Temperature: 20°C

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Submitted Aug 23 2007

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