Product nameAnti-SORBS2 antibody
See all SORBS2 primary antibodies
DescriptionMouse polyclonal to SORBS2
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Human
Full length (aa 1-1100) human SORBS2 protein (NP_066547.1).
- HepG2 cells; SORBS2 transfected 293T cell lysate.
Storage instructionsShipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.20
Constituent: 2.68% PBS
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab73444 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/1000. Detects a band of approximately 124 kDa (predicted molecular weight: 124 kDa).|
|ICC/IF||Use a concentration of 10 µg/ml.|
FunctionAdapter protein that plays a role in the assembling of signaling complexes, being a link between ABL kinases and actin cytoskeleton. Can form complex with ABL1 and CBL, thus promoting ubiquitination and degradation of ABL1 or with AKT1 and PAK1, thus mediating AKT1-mediated activation of PAK1. May play a role in the regulation of pancreatic cell adhesion, possibly by acting on WASF1 phosphorylation, enhancing phosphorylation by ABL1, as well as dephosphorylation by PTPN12 (PubMed:18559503). Isoform 6 increases water and sodium absorption in the intestine and gall-bladder.
Tissue specificityAbundantly expressed in heart. In cardiac muscle cells, located in the Z-disks of sarcomere. Also found, but to a lower extent, in small and large intestine, pancreas, thymus, colon, spleen, prostate, testis, brain, ovary and epithelial cells. In the pancreas, mainly expressed in acinar cells, duct cells and all cell types in islets (at protein level). Tends to be down-regulated in pancreatic adenocarcinomas ans metastases.
Sequence similaritiesContains 3 SH3 domains.
Contains 1 SoHo domain.
DomainThe first 2 SH3 domains are required for WASF1-binding. All 3 SH3 domains can bind independently to PTPN12.
modificationsUbiquitinated by CBL.
Dephosphorylated by PTPN12.
Cellular localizationCytoplasm, perinuclear region. Apical cell membrane. Cell junction, focal adhesion. Cell projection, lamellipodium. Found at the Z-disk sarcomeres, stress fibers, dense bodies and focal adhesion. In pancreatic acinar cells, localized preferentially to the apical membrane. Colocalized with vinculin and filamentous actin at focal adhesions and lamellipodia of pancreatic cells.
- Information by UniProt
- Arg binding protein 2 antibody
- Arg-binding protein 2 antibody
- Arg/Abl interacting protein 2 antibody
ab73444 at 10µg/ml staining SORBS2 in HepG2 cells by Immunofluorescence.
All lanes : Anti-SORBS2 antibody (ab73444) at 1/500 dilution
Lane 1 : SORBS2 transfected 293T cell lysate
Lane 2 : Non-transfected 293T cell lysate
Lysates/proteins at 25 µg per lane.
All lanes : Goat Anti-Mouse IgG (H&L)-HRP at 1/2500 dilution
Predicted band size: 124 kDa
Observed band size: 124 kDa
This product has been referenced in:
- Zhao L et al. The RNA binding protein SORBS2 suppresses metastatic colonization of ovarian cancer by stabilizing tumor-suppressive immunomodulatory transcripts. Genome Biol 19:35 (2018). Read more (PubMed: 29548303) »
- Kawabori M et al. Triggering receptor expressed on myeloid cells 2 (TREM2) deficiency attenuates phagocytic activities of microglia and exacerbates ischemic damage in experimental stroke. J Neurosci 35:3384-96 (2015). IHC-FoFr ; Mouse . Read more (PubMed: 25716838) »