• Product name
  • Description
    Rabbit polyclonal to SorLA
  • Host species
  • Tested applications
    Suitable for: IHC-P, IHC-Fr, IHC (PFA fixed)more details
  • Species reactivity
    Reacts with: Rat, Human
    Predicted to work with: Mouse
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 2150 to the C-terminus of Human SorLA.

    Read Abcam's proprietary immunogen policy (Peptide available as ab16691.)

  • Positive control
    • IHC-P: Rat brain tissue, most abundant expression has been identified in the cerebellum, cerebral cortex and the occipital pole.



Our Abpromise guarantee covers the use of ab16642 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr Use at an assay dependent concentration.
IHC (PFA fixed) Use a concentration of 0.6 - 1 µg/ml.


  • Function
    Likely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction.
  • Tissue specificity
    Expressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals.
  • Sequence similarities
    Belongs to the SORL1 family.
    Contains 5 BNR repeats.
    Contains 1 EGF-like domain.
    Contains 6 fibronectin type-III domains.
    Contains 11 LDL-receptor class A domains.
    Contains 5 LDL-receptor class B repeats.
  • Post-translational
    The propeptide removed in the N-terminus may be cleaved by furin or homologous proteases.
  • Cellular localization
  • Information by UniProt
  • Database links
  • Alternative names
    • C11orf32 antibody
    • FLJ21930 antibody
    • FLJ39258 antibody
    • gp250 antibody
    • LDLR relative with 11 ligand binding repeats antibody
    • LDLR relative with 11 ligand-binding repeats antibody
    • Low density lipoprotein receptor relative with 11 ligand binding repeats antibody
    • Low-density lipoprotein receptor relative with 11 ligand-binding repeats antibody
    • LR 11 antibody
    • LR11 antibody
    • LRP 9 antibody
    • LRP9 antibody
    • Mosaic protein LR11 antibody
    • SORL 1 antibody
    • SORL_HUMAN antibody
    • SORL1 antibody
    • SorLA 1 antibody
    • SorLA antibody
    • SorLA-1 antibody
    • Sortilin related receptor antibody
    • Sortilin related receptor L(DLR class) A repeats containing antibody
    • Sortilin-related receptor antibody
    • Sorting protein related receptor containing LDLR class A repeats antibody
    • Sorting protein-related receptor containing LDLR class A repeats antibody
    see all


  • Immunofluorescent staining for SorLA in rat cortical neurons using Rabbit polyclonal to SorLA (ab16642). The staining is cytoplasmic and punctate in these cortical neurons.

    Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab16642 was incubated overnight at 1/500 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.

  • Image courtesy of Human Protein Atlas
    ab16642 staining SorLA in paraffin embedded human cerebellum tissues, showing a strong signal in the granular and molecular cell layers and Purkinje cells. Paraffin embedded human cerebellum sections (4µm) were incubated with ab16642 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16642 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.


This product has been referenced in:
  • Gowrishankar S  et al. Massive accumulation of luminal protease-deficient axonal lysosomes at Alzheimer's disease amyloid plaques. Proc Natl Acad Sci U S A 112:E3699-708 (2015). Read more (PubMed: 26124111) »
  • Mecozzi VJ  et al. Pharmacological chaperones stabilize retromer to limit APP processing. Nat Chem Biol 10:443-9 (2014). Read more (PubMed: 24747528) »
See all 3 Publications for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate buffer
Mouse Tissue sections (Brain)

Abcam user community

Verified customer

Submitted Apr 02 2015


Thank you for your phone call.
As a one time exception I am sending you retroactively a new discount code. Please ensure to use it in the given time period.

Expiration date: xxx
I am very pleased to hear you would like to accept our offer and test ab16642 in WB. This code will give you: 1 free PRIMARY ANTIBODY before the expiration date. To redeem this offer, please submit an Abreview for WB and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. Please remember that submission of the Abreview is sufficient for the discount code to become active.
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Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.
Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.
The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

Read More
Abcam has not validated the combination of species/application used in this Abreview.
Western blot
Rat Tissue lysate - whole (Cortex)
Loading amount
30 µg
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Dr. Balmiki Ray

Verified customer

Submitted Jun 17 2010

Abcam guarantees this product to work in the species/application used in this Abreview.
Immunohistochemistry (Frozen sections)
Rat Tissue sections (sections of rat brain)
sections of rat brain

Dr. Sophie Pezet

Verified customer

Submitted Dec 21 2005


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