Product nameAnti-SorLA antibody
See all SorLA primary antibodies
DescriptionRabbit polyclonal to SorLA
Tested applicationsSuitable for: IHC-P, IHC-Fr, IHC (PFA fixed)more details
Species reactivityReacts with: Rat, Human
Predicted to work with: Mouse
- IHC-P: Rat brain tissue, most abundant expression has been identified in the cerebellum, cerebral cortex and the occipital pole.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab16642 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/40. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.|
|IHC-Fr||Use at an assay dependent concentration.|
|IHC (PFA fixed)||Use a concentration of 0.6 - 1 µg/ml.|
FunctionLikely to be a multifunctional endocytic receptor, that may be implicated in the uptake of lipoproteins and of proteases. Binds LDL, the major cholesterol-carrying lipoprotein of plasma, and transports it into cells by endocytosis. Binds the receptor-associated protein (RAP). Could play a role in cell-cell interaction.
Tissue specificityExpressed mainly in brain, where it is most abundant in the cerebellum, cerebral cortex and the occipital pole; low expression in the putamen and the thalamus. According to PubMed:9157966, found in spinal cord, testis, liver, kidney and pancreas with detectable levels in placenta, lung and heart. According to PubMed:8940146, expressed in the prostate, ovary, thyroid and spleen, but not found in kidney, liver, lung, skeletal muscle, bone marrow and adrenals.
Sequence similaritiesBelongs to the SORL1 family.
Contains 5 BNR repeats.
Contains 1 EGF-like domain.
Contains 6 fibronectin type-III domains.
Contains 11 LDL-receptor class A domains.
Contains 5 LDL-receptor class B repeats.
modificationsThe propeptide removed in the N-terminus may be cleaved by furin or homologous proteases.
- Information by UniProt
- C11orf32 antibody
- FLJ21930 antibody
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Immunofluorescent staining for SorLA in rat cortical neurons using Rabbit polyclonal to SorLA (ab16642). The staining is cytoplasmic and punctate in these cortical neurons.
Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, and cryoprotected in 20% sucrose and frozen in OCT. 30µm coronal sections were cut by cyrostat for use in fre floating IHC. Primary antibody ab16642 was incubated overnight at 1/500 at room temperature. Secondary antibody Alexa fluor 488 1/1000 was incubated for 2 hours at room temperature.
Image courtesy of Human Protein Atlas
ab16642 staining SorLA in paraffin embedded human cerebellum tissues, showing a strong signal in the granular and molecular cell layers and Purkinje cells. Paraffin embedded human cerebellum sections (4µm) were incubated with ab16642 (1/40 dilution) for 30 mins at room temperature. Antigen retrieval was performed by heat induction in citrate buffer pH 6. ab16642 was tested in a tissue microarray (TMA) containing a wide range of normal and cancer tissues as well as a cell microarray consisting of a range of commonly used, well characterised human cell lines. Further results for this antibody can be found at www.proteinatlas.org.
This product has been referenced in:
- Gowrishankar S et al. Massive accumulation of luminal protease-deficient axonal lysosomes at Alzheimer's disease amyloid plaques. Proc Natl Acad Sci U S A 112:E3699-708 (2015). Read more (PubMed: 26124111) »
- Mecozzi VJ et al. Pharmacological chaperones stabilize retromer to limit APP processing. Nat Chem Biol 10:443-9 (2014). Read more (PubMed: 24747528) »