Anti-Sortilin/NT3 antibody (ab16640)

Knockout Tested Rabbit polyclonal Sortilin/NT3 antibody. Validated in WB, IP, IHC, ICC, Flow Cyt, ICC/IF and tested in Mouse, Rat, Cow, Human. Cited in 48 publication(s).


  • Product name
    Anti-Sortilin/NT3 antibody
    See all Sortilin/NT3 primary antibodies
  • Description
    Rabbit polyclonal to Sortilin/NT3
  • Host species
  • Tested applications
    Suitable for: WB, ICC/IF, Flow Cyt, IP, ICC, IHC-FoFrmore details
  • Species reactivity
    Reacts with: Mouse, Rat, Cow, Human
  • Immunogen

    Synthetic peptide corresponding to Human Sortilin/NT3 aa 800 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab16686)

  • Positive control
    • Mouse, human or rat brain



Our Abpromise guarantee covers the use of ab16640 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 100 kDa (predicted molecular weight: 95 kDa).
ICC/IF Use a concentration of 1 µg/ml.
Flow Cyt Use at an assay dependent concentration. PubMed: 19761405

ab171870 - Rabbit polyclonal IgG, is suitable for use as an isotype control with this antibody.

IP Use at an assay dependent concentration. PubMed: 21357693
ICC 1/1000.
IHC-FoFr Use a concentration of 0.2 - 0.5 µg/ml.


  • Function
    Functions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
  • Tissue specificity
    Expressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
  • Involvement in disease
    Note=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction.
  • Sequence similarities
    Belongs to the VPS10-related sortilin family. SORT1 subfamily.
    Contains 9 BNR repeats.
  • Domain
    The N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
    The extracellular domain may be shed following protease cleavage in some cell types.
  • Post-translational
    The N-terminal propeptide is cleaved by furin and possibly other homologous proteases.
  • Cellular localization
    Membrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
  • Information by UniProt
  • Database links
  • Alternative names
    • 100 kDa NT receptor antibody
    • Glycoprotein 95 antibody
    • Gp 95 antibody
    • Gp95 antibody
    • LDLCQ6 antibody
    • Neurotensin receptor 3 antibody
    • NT 3 antibody
    • NT3 antibody
    • NTR 3 antibody
    • NTR3 antibody
    • OTTHUMP00000013784 antibody
    • SORT 1 antibody
    • SORT_HUMAN antibody
    • SORT1 (gene name) antibody
    • Sort1 antibody
    • Sortilin 1 antibody
    • Sortilin antibody
    see all


  • Lane 1: Wild-type HAP1 cell lysate (20 µg)
    Lane 2: Sortilin/NT3 knockout HAP1 cell lysate (20 µg)
    Lane 3: NIH/3T3 cell lysate (20 µg)
    Lane 4: 293T cell lysate (20 µg)
    Lanes 1 - 4: Merged signal (red and green). Green - ab16440 observed at 100 kDa. Red - loading control, ab8245, observed at 37 kDa.

    ab16640 was shown to specifically react with Sortilin/NT3 in wild-type HAP1 cells. No band was observed when Sortilin/NT3 knockout samples were examined. Wild-type and Sortilin/NT3 knockout samples were subjected to SDS-PAGE. ab16640 and ab8245 (loading control to GAPDH) were diluted 1 µg/mL and 1/10,000 respectively and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging.

  • Immunofluorescent staining for Sortilin/NT3 in rat cortical neurons using Rabbit polyclonal to Sortilin (ab16640). The staining is cytoplasmic and punctate of many cells such as cortical neurons. The image was taken with a X20 objective.

    Protocol details: Rats were intracardially perfused with 4% paraformaldehyde. Whole brain tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT and then cut on cryostat (30µm coronal sections). IHC was perfored in free floating with fixed tissues (rat brain sections). Primary antibody was incubated overnight at 1/3000 at room temperature

  • ab16640 staining cultured mouse astrocytes by ICC/IF. The cultured cells were fixed with 4% paraformaldehyde for 5 minutes and blocked with 10% donkey serum in 0.1% PBS-0.3% TritonX for 30 minutes at 24°C. The cultured cells were then stained with ab16640 at 1/1000 in 0.3% TritonX with 0.1% PBS and 10% donkey serum for 4h at 24°C. An Alexa Fluro 488 donkey anti-rabbit polyclonal antibody at 1/1000 was used as the secondary antibody. Nuclei were stained with 1.43µM Hoechst and can be observed in blue. Sortilin expressed in the cytosol mainly in trans-golgi compartments.

    See Abreview

  • Lane 1 : Marker
    Lanes 2-4 : Anti-Sortilin/NT3 antibody (ab16640) at 1 µg/ml

    Lane 2 : Human Brain Tissue Lysate at 20 µg

    Lane 3 : Mouse Brain at 20 µg

    Lane 4 : Brain (Rat) Whole Cell Lysate - normal tissue at 20 µg

    Lanes 2-4 : Goat polyclonal to Rabbit IgG H&L (HRP) Pre-Adsorbed at 1/10000 dilution

    Performed under reducing conditions.

    Predicted band size: 95 kDa
    Additional bands at: 31 kDa (possible cleavage fragment)

  • ICC/IF image of ab16640 stained PC12 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab16640, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.


This product has been referenced in:
See all 50 Publications for this product

Customer reviews and Q&As

1-8 of 8 Abreviews or Q&A


I am sorry this product did not perform as stated on the datasheet and for the inconvenience this has caused. As requested, I have issued a free of charge replacement with the order number ****.

To check the status of the order please contact our Customer Service team and reference this number.

Please note that this free of charge replacement vial is also covered by our Abpromise guarantee. Should you still be experiencing difficulties, or if you have any further questions, please do not hesitate to let us know.

I wish you the best of luck with your research.

Read More
Western blot
Rat Tissue lysate - whole (Rat brain-Hippocampus, cortex)
Gel Running Conditions
Non-reduced Denaturing (4-12 % Bis-Tris)
Loading amount
20 µg
Rat brain-Hippocampus, cortex
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Apr 30 2012

Mouse Cultured Cells (Astrocytes culture)
Yes - 0.3% TritonX in 0.1% PBS
Astrocytes culture
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Feb 13 2012

Immunocytochemistry/ Immunofluorescence
Rat Cultured Cells (Hippocampal neurons)
Yes - 0.3% TritonX in 0.1% PBS
Hippocampal neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 24°C

Dr. Ruma Raha-Chowdhury

Verified customer

Submitted Sep 02 2011

Western blot
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Reduced Denaturing
Loading amount
40 µg
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Mr. Youngjeon Lee

Verified customer

Submitted Dec 29 2009

Western blot
Human Cell lysate - whole cell (Schwann Cells)
Loading amount
45 µg
Schwann Cells
Blocking step
Milk as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 5%

Matthew Provenzano

Verified customer

Submitted Mar 27 2007


Thank you for getting back to me and for sharing your recent findings with me. I am very sorry to hear that even after following my suggestions, you are still not satisfied with this antibody. This product has been tested for IHC by one of our collaborators who has used rat cortical neurons fixed with 4% paraformaldehyde. Rats were intracardially perfused with 4% paraformaldehyde and the whole brain tissue was post-fixed overnight in the same fixative, cryoprotected in 20% sucrose and frozen in OCT and then cut on cryostat (30µm coronal sections). IHC was performed in free floating with fixed tissues (rat brain sections). As I understand from your previous e-mail, you tried 1% formaldehyde, 1% paraformaldehyde, 4% paraformaldehyde for 20 minutes. As I have mentioned ab16640 has not been tested for ICC/IF, only for IHC on perfused samples and it is very likely that the different fixation technique may be the reason for this discrepancy. My last suggestion, perhaps, would be to test ice-cold methanol or acetone (only for 5 mins) as alternative fixation to see if it changes the staining pattern. Since you purchased this antibody in September and the guarantee period (as Abcam promise indicates) has not expired, I am ready to offer you a new vial as a free of charge replacement – in case the product may have been damaged during storing or shipping. Please do let me know if you would like to get one vial of this product to test or not. Your findings are very interesting and I am convinced that other customers would find your feedback very useful. We always encourage customers to send their results back to us, whether positive or negative, and make all product information available to researchers. If you send us an Abreview, we can offer you from 50 up to 300 Abcam points (Abpoints) based on submission time and type of application. The less characterised the product you review, the more Abpoints you will receive. Furthermore, you get 100 extra Abpoints for submitting an image. These Abpoints can then be claimed back as rewards. To find out more about our new Loyalty Scheme, Abpoints and rewards please visit our website at and look at Your Account panel (left bottom section on our home page). You can submit the Abreview if you click on the Abreview section (top menu bar) on the on-line datasheet of this antibody. It is a very straightforward process. Should you need any help with your submission, then please do not hesitate to contact us again. Thank you for contacting Abcam again and I am looking forward to hearing from you.

Read More
Immunohistochemistry (PFA perfusion fixed frozen sections)
Rat Tissue sections (Rat brain)
Rat brain
Antigen retrieval step

Dr. Sophie Pezet

Verified customer

Submitted Nov 02 2006

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