Recombinant Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)
- Datasheet
- References (1)
- Protocols
Overview
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Product nameAnti-Sortilin/NT3 antibody [EPR15010]
See all Sortilin/NT3 primary antibodies -
DescriptionRabbit monoclonal [EPR15010] to Sortilin/NT3
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Host speciesRabbit
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Tested applicationsSuitable for: IHC-P, WBmore details
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Species reactivityReacts with: Human
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Immunogen
Recombinant fragment within Human Sortilin/NT3 aa 650-800. The exact sequence is proprietary.
Database link: Q99523 -
Positive control
- WB: SW480 whole cell lysate (ab3957), SW40, HeLa and SH-SY5Y cell lysates. IHC-P: Human brain tissues.
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General notes
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
This product is a recombinant rabbit monoclonal antibody.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
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Storage bufferPreservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA -
Concentration information loading... -
PurityProtein A purified
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ClonalityMonoclonal
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Clone numberEPR15010
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IsotypeIgG
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab188586 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
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| IHC-P | 1/250. Perform heat mediated antigen retrieval with EDTA Buffer, pH9 before commencing with IHC protocol. |
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| WB | 1/1000 - 1/2000. Detects a band of approximately 92-114 kDa (predicted molecular weight: 92 kDa). |
Target
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FunctionFunctions as a sorting receptor in the Golgi compartment and as a clearance receptor on the cell surface. Required for protein transport from the Golgi apparatus to the lysosomes by a pathway that is independent of the mannose-6-phosphate receptor (M6PR). Also required for protein transport from the Golgi apparatus to the endosomes. Promotes neuronal apoptosis by mediating endocytosis of the proapoptotic precursor forms of BDNF (proBDNF) and NGFB (proNGFB). Also acts as a receptor for neurotensin. May promote mineralization of the extracellular matrix during osteogenic differentiation by scavenging extracellular LPL. Probably required in adipocytes for the formation of specialized storage vesicles containing the glucose transporter SLC2A4/GLUT4 (GLUT4 storage vesicles, or GSVs). These vesicles provide a stable pool of SLC2A4 and confer increased responsiveness to insulin. May also mediate transport from the endoplasmic reticulum to the Golgi.
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Tissue specificityExpressed at high levels in brain, spinal cord, heart, skeletal muscle, thyroid, placenta and testis. Expressed at lower levels in lymphoid organs, kidney, colon and liver.
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Involvement in diseaseNote=A common polymorphism located in a non-coding region between CELSR2 and PSRC1 alters a CEBP transcription factor binding site and is responsible for changes in hepatic expression of SORT1. Altered SORT1 expression in liver affects low density lipoprotein cholesterol levels in plasma and is associated with susceptibility to myocardial infarction.
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Sequence similaritiesBelongs to the VPS10-related sortilin family. SORT1 subfamily.
Contains 9 BNR repeats. -
DomainThe N-terminal propeptide may facilitate precursor transport within the Golgi stack. Intrachain binding of the N-terminal propeptide and the extracellular domain may also inhibit premature ligand binding.
The extracellular domain may be shed following protease cleavage in some cell types. -
Post-translational
modificationsThe N-terminal propeptide is cleaved by furin and possibly other homologous proteases. -
Cellular localizationMembrane. Endoplasmic reticulum membrane. Endosome membrane. Golgi apparatus > Golgi stack membrane. Lysosome membrane. Nucleus membrane. Cell membrane. Lysosome membrane. Localized to membranes of the endoplasmic reticulum, endosomes, Golgi stack, lysosomes and nucleus. A small fraction of the protein is also localized to the plasma membrane. May also be found in SLC2A4/GLUT4 storage vesicles (GSVs) in adipocytes. Localization to the plasma membrane in adipocytes may be enhanced by insulin.
- Information by UniProt
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Database links
- Entrez Gene: 6272 Human
- Omim: 602458 Human
- SwissProt: Q99523 Human
- Unigene: 485195 Human
- Unigene: 703487 Human
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Alternative names
- 100 kDa NT receptor antibody
- Glycoprotein 95 antibody
- Gp 95 antibody
see all
Images
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![Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)](https://www.abcam.com/ps/products/188/ab188586/Images/ab188586-254422-ab188586WTKO.jpg)
Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)Lane 1: Wild-type HAP1 cell lysate (20 µg)
Lane 2: Sortilin/NT3 knockout HAP1 cell lysate (20 µg)
Lane 3: SW480 cell lysate (20 µg)
Lane 4: HeLa cell lysate (20 µg)Lanes 1 - 4: Merged signal (red and green). Green - ab188586 observed at 92 kDa. Red - loading control, ab8245, observed at 37 kDa.
ab188586 was shown to react with Sortilin/NT3 in wild-type HAP1 cells along with additional cross-reactive bands. No band was observed when Sortilin/NT3 knockout samples were examined. Wild-type and Sortilin/NT3 knockout samples were subjected to SDS-PAGE. ab188586 and ab8245 (loading control to GAPDH) were both diluted 1/2000 and incubated overnight at 4°C. Blots were developed with Goat anti-Rabbit IgG H&L (IRDye® 800CW) preadsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye® 680RD) preadsorbed (ab216776) secondary antibodies at 1/10,000 dilution for 1 hour at room temperature before imaging. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)](https://www.abcam.com/ps/products/188/ab188586/Images/ab188586-222650-ab188586c.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)Immunohistochemical analysis of paraffin-embedded Human brain tissue labeling Sortilin/NT3 with ab188586 at 1/250 dilution followed by prediluted HRP Polymer for Rabbit IgG. Counter stained with Hematoxylin.
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![Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)](https://www.abcam.com/ps/products/188/ab188586/Images/ab188586-284707-ab188586wb.jpg)
Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)All lanes : Anti-Sortilin/NT3 antibody [EPR15010] (ab188586) at 1/1000 dilution
Lane 1 : SH-SY5Y whole cell lysate
Lane 2 : Human hippocampus tissue lysate
Lane 3 : Human fetal brain tissue lysate
Lane 4 : Mouse brain tissue lysate
Lane 5 : 3T3-L1 whole cell lysate
Lane 6 : Rat brain tissue lysate
Lysates/proteins at 15 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 92 kDa
Observed band size: 92 kDa
Exposure time: 30 secondsBlocking and dilution buffer: 5% NFDM/TBST.
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![Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)](https://www.abcam.com/ps/products/188/ab188586/Images/ab188586-222652-188586.JPG)
Western blot - Anti-Sortilin/NT3 antibody [EPR15010] (ab188586)All lanes : Anti-Sortilin/NT3 antibody [EPR15010] (ab188586) at 1/2000 dilution
Lane 1 : SW480 cell lysate
Lane 2 : HeLa cell lysate
Lane 3 : SH-SY5Y cell lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugate at 1/1000 dilution
Predicted band size: 92 kDa
Datasheets and documents
References
This product has been referenced in:
- Hirst J et al. Role of the AP-5 adaptor protein complex in late endosome-to-Golgi retrieval. PLoS Biol 16:e2004411 (2018). Read more (PubMed: 29381698) »
