Key features and details
- Rabbit polyclonal to SOS1
- Suitable for: IP, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-SOS1 antibody
See all SOS1 primary antibodies
DescriptionRabbit polyclonal to SOS1
Tested applicationsSuitable for: IP, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse
Synthetic peptide within Human SOS1 aa 1283-1333. The exact sequence is proprietary. NP_005624.2
Database link: Q07889
- WB: HeLa and HEK-293T whole cell lysate. IP: HeLa whole cell lysate.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 6.8
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab245645 was affinity purified using an epitope specific to SOS1 immobilized on solid support.
Our Abpromise guarantee covers the use of ab245645 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at 2-5 µg/mg of lysate.|
|WB||1/2000 - 1/10000. Predicted molecular weight: 152 kDa.|
FunctionPromotes the exchange of Ras-bound GDP by GTP.
Tissue specificityExpressed in gingival tissues.
Involvement in diseaseDefects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
Sequence similaritiesContains 1 DH (DBL-homology) domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PH domain.
Contains 1 Ras-GEF domain.
- Information by UniProt
- alternate SOS1 antibody
- GF1 antibody
- GGF1 antibody
All lanes : Anti-SOS1 antibody (ab245645) at 0.04 µg/ml
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 152 kDa
Exposure time: 3 minutes
SOS1 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1 mg per IP reaction; 20% of IP loaded).
ab245645 used for IP at 3 µg/mg lysate. For WB 1 µg/ml.
Lane 1: ab245645 IP in HeLa whole cell lysate.
Lane 2: Control IgG in HeLa whole cell lysate.
Chemiluminescence detection: 30 seconds.
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab245645 has not yet been referenced specifically in any publications.