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RecombinantRabMAb

Recombinant Anti-SOS1 antibody [EPR7480] (ab140621)

  • Datasheet
  • SDS
Reviews (3) Submit a question References (2)

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Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
  • Anti-SOS1 antibody [EPR7480] (ab140621)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR7480] to SOS1
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-SOS1 antibody [EPR7480]
    See all SOS1 primary antibodies
  • Description

    Rabbit monoclonal [EPR7480] to SOS1
  • Host species

    Rabbit
  • Tested Applications & Species

    Application Species
    ICC/IF
    Human
    IHC-P
    Human
    WB
    Human
    See all applications and species data
  • Immunogen

    Recombinant fragment corresponding to Human SOS1 aa 1050-1200.

  • Positive control

    • Raji, K562, HeLa and THP1 cell lysates; Human ovarian carcinoma tissue; Raji cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at -20ºC.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR7480
  • Isotype

    IgG
  • Research areas

    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Regulators
    • Signal Transduction
    • Signaling Pathway
    • G Protein Signaling
    • Small G Proteins
    • Ras Family
    • Cancer
    • Signal transduction
    • G protein signaling
    • Small G proteins
    • Ras family

Associated products

  • Alternative Versions

    • Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Positive Controls

    • HeLa whole cell lysate (ab29545)
    • Raji whole cell lysate (ab30124)
    • K-562 whole cell lysate (ab7911)
    • THP-1 whole cell lysate (ab7913)
  • Recombinant Protein

    • Recombinant Human SOS1 protein (His tag) (ab233594)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
ICC/IF
Human
IHC-P
Human
WB
Human
All applications
Mouse
Rat
Application Abreviews Notes
WB (1)
1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P (2)
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
Notes
WB
1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P
1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF
1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function

      Promotes the exchange of Ras-bound GDP by GTP.
    • Tissue specificity

      Expressed in gingival tissues.
    • Involvement in disease

      Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
      Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
    • Sequence similarities

      Contains 1 DH (DBL-homology) domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 PH domain.
      Contains 1 Ras-GEF domain.
    • Target information above from: UniProt accession Q07889 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6654 Human
      • Entrez Gene: 20662 Mouse
      • Entrez Gene: 313845 Rat
      • Omim: 182530 Human
      • SwissProt: Q07889 Human
      • SwissProt: Q62245 Mouse
      • Unigene: 709893 Human
      • Unigene: 360004 Mouse
      • Unigene: 60975 Mouse
      • Unigene: 91844 Rat
      see all
    • Alternative names

      • alternate SOS1 antibody
      • GF1 antibody
      • GGF1 antibody
      • GINGF antibody
      • gingival fibromatosis antibody
      • gingival fibromatosis hereditary 1 antibody
      • Guanine nucleotide exchange factor antibody
      • HGF antibody
      • NS4 antibody
      • Son of sevenless homolog 1 (Drosophila) antibody
      • Son of sevenless homolog 1 antibody
      • SOS Ras/Rac guanine nucleotide exchange factor 1 antibody
      • SOS-1 antibody
      • Sos1 antibody
      • SOS1_HUMAN antibody
      see all

    Images

    • Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
      Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)
      All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 152 kDa

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

      Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.
    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

      Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

      Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

      Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

      Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

      Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.

      Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

    • Anti-SOS1 antibody [EPR7480] (ab140621)
      Anti-SOS1 antibody [EPR7480] (ab140621)

    Protocols

    • Western blot protocols
    • Immunohistochemistry protocols
    • Immunocytochemistry & immunofluorescence protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
    • SDS
  • References (2)

    Publishing research using ab140621? Please let us know so that we can cite the reference in this datasheet.

    ab140621 has been referenced in 2 publications.

    • Mitsuda Y  et al. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci Rep 8:6423 (2018). PubMed: 29686309
    • Naidu S  et al. PDGFR-modulated miR-23b cluster and miR-125a-5p suppress lung tumorigenesis by targeting multiple components of KRAS and NF-kB pathways. Sci Rep 7:15441 (2017). PubMed: 29133857

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-3 of 3 Abreviews or Q&A

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (A549 tumor)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: Citrate pH6
    Permeabilization
    No
    Specification
    A549 tumor
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Nov 03 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Prostate cancer)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: pH9 target retrieval Dako
    Permeabilization
    No
    Specification
    Prostate cancer
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 01 2020

    Western blot abreview for Anti-SOS1 antibody [EPR7480]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Human Cell lysate - other (HeLa and HEK293E)
    Gel Running Conditions
    Reduced Denaturing (3-8% Tris acetate)
    Loading amount
    3e+007 cells
    Treatment
    EGF and an inhibitor
    Specification
    HeLa and HEK293E
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
    Read More

    Stacy Wilson

    Verified customer

    Submitted Dec 19 2019

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