Overview

  • Product name
    Anti-SOS1 antibody [EPR7480]
    See all SOS1 primary antibodies
  • Description
    Rabbit monoclonal [EPR7480] to SOS1
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WB, IHC-P, ICC/IFmore details
    Unsuitable for: Flow Cyt or IP
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Recombinant fragment corresponding to amino acids in Human SOS1 (UniProt ID: Q07889).

  • Positive control
    • Raji, K562, HeLa and THP1 cell lysates; Human ovarian carcinoma tissue; Raji cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab140621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.
  • Application notes
    Is unsuitable for Flow Cyt or IP.
  • Target

    • Function
      Promotes the exchange of Ras-bound GDP by GTP.
    • Tissue specificity
      Expressed in gingival tissues.
    • Involvement in disease
      Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
      Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
    • Sequence similarities
      Contains 1 DH (DBL-homology) domain.
      Contains 1 N-terminal Ras-GEF domain.
      Contains 1 PH domain.
      Contains 1 Ras-GEF domain.
    • Information by UniProt
    • Database links
    • Alternative names
      • alternate SOS1 antibody
      • GF1 antibody
      • GGF1 antibody
      • GINGF antibody
      • gingival fibromatosis antibody
      • gingival fibromatosis hereditary 1 antibody
      • Guanine nucleotide exchange factor antibody
      • HGF antibody
      • NS4 antibody
      • Son of sevenless homolog 1 (Drosophila) antibody
      • Son of sevenless homolog 1 antibody
      • SOS Ras/Rac guanine nucleotide exchange factor 1 antibody
      • SOS-1 antibody
      • Sos1 antibody
      • SOS1_HUMAN antibody
      see all

    Images

    • All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

      Lane 1 : Raji cell lysate
      Lane 2 : K562 cell lysate
      Lane 3 : HeLa cell lysate
      Lane 4 : THP1 cell lysate

      Lysates/proteins at 10 µg per lane.

      Predicted band size: 152 kDa

    • Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.
    • Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.
    • Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.

    • Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.

    References

    This product has been referenced in:
    • Mitsuda Y  et al. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci Rep 8:6423 (2018). Read more (PubMed: 29686309) »
    • Naidu S  et al. PDGFR-modulated miR-23b cluster and miR-125a-5p suppress lung tumorigenesis by targeting multiple components of KRAS and NF-kB pathways. Sci Rep 7:15441 (2017). Read more (PubMed: 29133857) »
    See all 2 Publications for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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