Name: Anti-SOS1 antibody [EPR7480]
Brand: RabMAb
SKU: ab140621
Rating: 5 (3 reviews)

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Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

Key features and details

Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
Rabbit monoclonal [EPR7480] to SOS1
Suitable for: WB, IHC-P, ICC/IF
Knockout validated
Reacts with: Human

Carrier Free

Product name

Anti-SOS1 antibody [EPR7480]
See all SOS1 primary antibodies
Description

Rabbit monoclonal [EPR7480] to SOS1
Host species

Rabbit
Tested applications

Suitable for: WB, IHC-P, ICC/IFmore details
Unsuitable for: Flow Cyt or IP
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat
Immunogen

Recombinant fragment corresponding to Human SOS1 aa 1050-1200.
Positive control
- Raji, K562, HeLa and THP1 cell lysates; Human ovarian carcinoma tissue; Raji cells.
General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
For more information see here.
Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb^® patents.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at -20ºC.
Storage buffer

pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

EPR7480
Isotype

IgG
Research areas

Alternative Versions
- Anti-SOS1 antibody [EPR7480] - BSA and Azide free (ab248908)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Positive Controls
Recombinant Protein
- Recombinant Human SOS1 protein (His tag) (ab233594)

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab140621 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
WB	(1)	1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P	(2)	1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF		1/250 - 1/500.

Notes
WB 1/1000 - 1/10000. Predicted molecular weight: 152 kDa.
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/250 - 1/500.

Application notes

Is unsuitable for Flow Cyt or IP.

Function

Promotes the exchange of Ras-bound GDP by GTP.
Tissue specificity

Expressed in gingival tissues.
Involvement in disease

Defects in SOS1 are the cause of gingival fibromatosis 1 (GGF1) [MIM:135300]; also known as GINGF1. Gingival fibromatosis is a rare overgrowth condition characterized by a benign, slowly progressive, nonhemorrhagic, fibrous enlargement of maxillary and mandibular keratinized gingiva. GGF1 is usually transmitted as an autosomal dominant trait, although sporadic cases are common.
Defects in SOS1 are the cause of Noonan syndrome type 4 (NS4) [MIM:610733]. NS4 is an autosomal dominant disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS4 is associated with juvenile myelomonocytic leukemia (JMML). SOS1 mutations engender a high prevalence of pulmonary valve disease; atrial septal defects are less common.
Sequence similarities

Contains 1 DH (DBL-homology) domain.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 PH domain.
Contains 1 Ras-GEF domain.
Target information above from: UniProt accession Q07889 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6654 Human
- Entrez Gene: 20662 Mouse
- Entrez Gene: 313845 Rat
- Omim: 182530 Human
- SwissProt: Q07889 Human
- SwissProt: Q62245 Mouse
- Unigene: 709893 Human
- Unigene: 360004 Mouse
- Unigene: 60975 Mouse
see all
Alternative names
- alternate SOS1 antibody
- GF1 antibody
- GGF1 antibody
- GINGF antibody
- gingival fibromatosis antibody
- gingival fibromatosis hereditary 1 antibody
- Guanine nucleotide exchange factor antibody
- HGF antibody
- NS4 antibody
- Son of sevenless homolog 1 (Drosophila) antibody
- Son of sevenless homolog 1 antibody
- SOS Ras/Rac guanine nucleotide exchange factor 1 antibody
- SOS-1 antibody
- Sos1 antibody
- SOS1_HUMAN antibody
see all

Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

Lane 1 : Wild-type A431 cell lysate
Lane 2 : SOS1 knockout A431 cell lysate
Lane 3 : HEK-293 cell lysate
Lane 4 : SK-OV-3 cell lysate

Lysates/proteins at 20 µg per lane.

Performed under reducing conditions.

Predicted band size: 152 kDa
Observed band size: 171 kDa why is the actual band size different from the predicted?

False colour image of Western blot: Anti-SOS1 antibody [EPR7480] staining at 1/1000 dilution, shown in green; Mouse anti-Alpha Tubulin [DM1A] (ab7291) loading control staining at 1/20000 dilution, shown in red. In Western blot, ab140621 was shown to bind specifically to SOS1. A band was observed at 171 kDa in wild-type A431 cell lysates with no signal observed at this size in SOS1 knockout cell line ab276087 (knockout cell lysate ab283833). To generate this image, wild-type and SOS1 knockout A431 cell lysates were analysed. First, samples were run on an SDS-PAGE gel then transferred onto a nitrocellulose membrane. Membranes were blocked in 3 % milk in TBS-0.1 % Tween^Â® 20 (TBS-T) before incubation with primary antibodies overnight at 4 Â°C. Blots were washed four times in TBS-T, incubated with secondary antibodies for 1 h at room temperature, washed again four times then imaged. Secondary antibodies used were Goat anti-Rabbit IgG H&L (IRDye^Â® 800CW) preabsorbed (ab216773) and Goat anti-Mouse IgG H&L (IRDye^Â® 680RD) preabsorbed (ab216776) at 1/20000 dilution.
Western blot - Anti-SOS1 antibody [EPR7480] (ab140621)

All lanes : Anti-SOS1 antibody [EPR7480] (ab140621) at 1/1000 dilution

Lane 1 : Raji cell lysate
Lane 2 : K562 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : THP1 cell lysate

Lysates/proteins at 10 µg per lane.

Predicted band size: 152 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin-embedded Human ovarian carcinoma tissue labelling SOS1 with ab140621 at 1/100 dilution.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunocytochemistry/ Immunofluorescence - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunofluorescent staining of Raji cells labelling SOS1 with ab140621 at 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Breast carcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded normal Human tonsil tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Lung adenocarcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Glioma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOS1 antibody [EPR7480] (ab140621)

Immunohistochemical analysis of paraffin embedded Human Cervical carcinoma tissue using ab140621 showing +ve staining.

Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Anti-SOS1 antibody [EPR7480] (ab140621)

Western blot protocols
Immunohistochemistry protocols
Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

SDS download

Country/Region

Language
Datasheet download

Download

Publishing research using ab140621? Please let us know so that we can cite the reference in this datasheet.

ab140621 has been referenced in 4 publications.

Cowan JR et al. SOS1 Gain-of-Function Variants in Dilated Cardiomyopathy. Circ Genom Precis Med 13:e002892 (2020). PubMed: 32603605
Mishra M et al. Adaptor Protein p66Shc: A Link Between Cytosolic and Mitochondrial Dysfunction in the Development of Diabetic Retinopathy. Antioxid Redox Signal 30:1621-1634 (2019). PubMed: 30105917
Mitsuda Y et al. RUNX1 positively regulates the ErbB2/HER2 signaling pathway through modulating SOS1 expression in gastric cancer cells. Sci Rep 8:6423 (2018). PubMed: 29686309
Naidu S et al. PDGFR-modulated miR-23b cluster and miR-125a-5p suppress lung tumorigenesis by targeting multiple components of KRAS and NF-kB pathways. Sci Rep 7:15441 (2017). PubMed: 29133857

Customer reviews and Q&As

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1-3 of 3 Abreviews or Q&A

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (A549 tumor)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: Citrate pH6

Permeabilization

Specification

A549 tumor

Blocking step

Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 20°C

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Nov 03 2020

Application

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)

Sample

Human Tissue sections (Prostate cancer)

Antigen retrieval step

Heat mediated - Buffer/Enzyme Used: pH9 target retrieval Dako

Permeabilization

Specification

Prostate cancer

Fixative

Formaldehyde

Abcam user community

Verified customer

Submitted Mar 01 2020

Application

Western blot

Sample

Human Cell lysate - other (HeLa and HEK293E)

Gel Running Conditions

Reduced Denaturing (3-8% Tris acetate)

Loading amount

3e+007 cells

Treatment

EGF and an inhibitor

Specification

HeLa and HEK293E

Blocking step

BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C

Stacy Wilson

Verified customer

Submitted Dec 19 2019

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Key features and details

Related conjugates and formulations

Overview

Product name

Description

Host species

Tested applications

Species reactivity

Immunogen

Positive control

General notes

Properties

Form

Storage instructions

Storage buffer

Purity

Clonality

Clone number

Isotype

Research areas

Associated products

Alternative Versions

Isotype control

Positive Controls

Recombinant Protein

Applications

The Abpromise guarantee

Target

Function

Tissue specificity

Involvement in disease

Sequence similarities

Database links

Alternative names

Images

Protocols

Datasheets and documents

SDS download

Datasheet download

References (4)

Customer reviews and Q&As

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOS1 antibody [EPR7480]

Western blot abreview for Anti-SOS1 antibody [EPR7480]