• Product name

  • Description

    Rabbit polyclonal to SOSTDC1
  • Host species

  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Macaque monkey, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human SOSTDC1 aa 150 to the C-terminus conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab119134)

  • Positive control

    • This antibody gave a positive signal in Human, Mouse and Rat Kidney tissue lysates. It also gave a positive signal in FFPE human normal lung tissue sections


  • Form

  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.40
    Preservative: 0.02% Sodium azide
    Constituent: PBS
    Note: Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

  • Isotype

  • Research areas


Our Abpromise guarantee covers the use of ab99340 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 28 kDa (predicted molecular weight: 23 kDa).
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.


  • Function

    May be involved in the onset of endometrial receptivity for implantation/sensitization for the decidual cell reaction Enhances Wnt signaling and inhibits TGF-beta signaling (By similarity). Directly antagonizes activity of BMP2, BMP4, BMP6 and BMP7 in a dose-dependent manner.
  • Tissue specificity

    Highly expressed in kidney and weakly in lung.
  • Sequence similarities

    Belongs to the sclerostin family.
    Contains 1 CTCK (C-terminal cystine knot-like) domain.
  • Cellular localization

  • Information by UniProt
  • Database links

  • Alternative names

    • CDA019 antibody
    • DKFZp564D206 antibody
    • Ectodermal BMP inhibitor antibody
    • Ectodin antibody
    • sclerostin domain containing 1 antibody
    • Sclerostin domain containing protein 1 antibody
    • Sclerostin domain-containing protein 1 antibody
    • SOSD1_HUMAN antibody
    • Sostdc1 antibody
    • USAG-1 antibody
    • USAG1 antibody
    • Uterine sensitization associated gene 1 protein antibody
    • Uterine sensitization-associated gene 1 protein antibody
    • Wise antibody
    see all


  • IHC image of SOSTDC1 staining in human normal lung formalin fixed paraffin embedded tissue section, performed on a Leica BondTM system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab99340, 5µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
  • All lanes : Anti-SOSTDC1 antibody (ab99340) at 1 µg/ml

    Lane 1 : Human kidney tissue lysate - total protein (ab30203)
    Lane 2 : Kidney (Mouse) Tissue Lysate
    Lane 3 : Kidney (Rat) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    All lanes : Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 23 kDa
    Observed band size: 28 kDa
    why is the actual band size different from the predicted?

    Exposure time: 20 minutes

    SOSTDC1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
  • ICC/IF image of ab99340 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab99340, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 4% formaldehyde fixed (10 min) HeLa cells at 5µg/ml, and in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.


This product has been referenced in:

  • Lu L  et al. Febuxostat inhibits TGF-ß1-induced epithelial-mesenchymal transition via downregulation of USAG-1 expression in Madin-Darby canine kidney cells in vitro. Mol Med Rep 19:1694-1704 (2019). Read more (PubMed: 30628645) »
  • Barbe MF  et al. Prolonged high force high repetition pulling induces osteocyte apoptosis and trabecular bone loss in distal radius, while low force high repetition pulling induces bone anabolism. Bone 110:267-283 (2018). IHC-P . Read more (PubMed: 29476978) »
See all 7 Publications for this product

Customer reviews and Q&As

1-6 of 6 Abreviews or Q&A

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Mouse Tissue sections (pancreas)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate
Yes - tritonX100
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Nov 05 2012


Gracias por enviarnos el Abreview.

Esta mañana lo he publicado en nuestra página web, con las imágenes incluidas. Ha quedado muy bien el montaje, gracias por molestarte.

El código puedes utilizarlo ya. Realiza tu pedido preferiblemente por mail, a mailto:orders@abcam.com indicando el código de descuento valido por un nuevo producto.

Si tienes cualquier otra consulta, no dudes en volverme a contactar.

Que pases una buena semana.

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¡Gracias a ti!

No olvides una vez enviados tus resultados a nuestra web, hacer el nuevo pedido indicando el código promocional para obtener un 100% de descuento.

Si necesitas mas información no dudes en contactarnos, por mail, o por teléfono.

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Gracias por tu respuesta.

Sí que sería válido para testarlo en immunohistoquímica. Para poder mandarte el código es necesario indicar también si las secciones a teñir están congeladas o embebidas en parafina.

Una vez me lo indiques te envío el código para que puedas testarlo y mandarnos tu publicación.

Muchas gracias.

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