Anti-SOX1 antibody (ab87775)
Key features and details
- Rabbit polyclonal to SOX1
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
- Long-term and scalable supply – powered by recombinant technology for fast production
- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SOX1 antibody
See all SOX1 primary antibodies -
Description
Rabbit polyclonal to SOX1 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Rabbit, Dog, Chimpanzee, Orangutan -
Immunogen
Synthetic peptide corresponding to Human SOX1 aa 350 to the C-terminus conjugated to keyhole limpet haemocyanin.
(Peptide available asab138973) -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.40
Preservative: 0.02% Sodium azide
Constituent: PBS
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help. -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab87775 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ICC/IF | (5) |
Use at an assay dependent concentration.
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IHC-P | (2) |
Use at an assay dependent concentration.
|
WB | (1) |
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
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Notes |
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ICC/IF
Use at an assay dependent concentration. |
IHC-P
Use at an assay dependent concentration. |
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). |
Target
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Function
Transcriptional activator. -
Tissue specificity
Mainly expressed in the developing central nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
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Alternative names
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
see all
Images
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All lanes : Anti-SOX1 antibody (ab87775) at 1 µg/ml
Lane 1 : E14Tg2a (Mouse embryonic stem cell line) Whole Cell Lysate
Lane 2 : 46C (Mouse embryonic stem cell line) Whole Cell Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 40 kDa why is the actual band size different from the predicted?
Additional bands at: 45 kDa (possible non-specific binding)
Exposure time: 8 minutesThis blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% BSA before being incubated with ab87775 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.
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All lanes : Anti-SOX1 antibody (ab87775) at 1/1000 dilution
Lane 1 : H1 Human embryonic stem cells
Lane 2 : H1 Human embryonic stem cell derived neural progenitor stem cells
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Mouse anti-rabbit HRP conjugated at 1/20000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 39 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Exposure time: 30 seconds
Datasheets and documents
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SDS download
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Datasheet download
References (22)
ab87775 has been referenced in 22 publications.
- Aslam N et al. Anti-oncogenic activities exhibited by paracrine factors of MSCs can be mediated by modulation of KITLG and DKK1 genes in glioma SCs in vitro. Mol Ther Oncolytics 20:147-165 (2021). PubMed: 33575478
- Ju ZH et al. Neurons derived from human-induced pluripotent stem cells express mu and kappa opioid receptors. Neural Regen Res 16:653-658 (2021). PubMed: 33063716
- Latchoumane CV et al. Engineered glycomaterial implants orchestrate large-scale functional repair of brain tissue chronically after severe traumatic brain injury. Sci Adv 7:N/A (2021). PubMed: 33674306
- Sharma Y et al. In vitro human stem cell derived cultures to monitor calcium signaling in neuronal development and function. Wellcome Open Res 5:16 (2020). PubMed: 32195361
- Li X et al. Odontogenesis and neuronal differentiation characteristics of periodontal ligament stem cells from beagle dog. J Cell Mol Med 24:5146-5151 (2020). PubMed: 32202359