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  1. Link

    sox1-antibody-epr23041-60-ab242125.pdf

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Epigenetics and Nuclear Signaling Transcription Domain Families HMG Box
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RecombinantRabMAb

Recombinant Anti-SOX1 antibody [EPR23041-60] (ab242125)

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  • SDS
  • Certificate of Compliance
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
  • Anti-SOX1 antibody [EPR23041-60] (ab242125)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR23041-60] to SOX1
  • Suitable for: IHC-P, IHC-Fr
  • Reacts with: Mouse, Rat

Conjugates logo Related conjugates and formulations

Carrier Free

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Primary
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Anti-PAX6 antibody [EPR3352(2)] (ab109233)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
Peptide
PAX6 peptide (ab5895)

View more associated products

Overview

  • Product name

    Anti-SOX1 antibody [EPR23041-60]
    See all SOX1 primary antibodies
  • Description

    Rabbit monoclonal [EPR23041-60] to SOX1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-Frmore details
    Unsuitable for: IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat
    Predicted to work with: Hamster
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Mouse cerebellum and hippocampus tissue; rat cerebellum tissue. IHC-Fr: Mouse and rat cerebellum tissue.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.2
    Preservative: 0.01% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    EPR23041-60
  • Isotype

    IgG
  • Research areas

    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Neuroscience
    • Neurology process
    • Neurogenesis
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Other factors
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • Developmental Families
    • Other
    • Stem Cells
    • Neural Stem Cells
    • Intracellular

Associated products

  • Alternative Versions

    • Anti-SOX1 antibody [EPR23041-60] - BSA and Azide free (ab263870)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Rabbit specific IHC polymer detection kit HRP/DAB (ab209101)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab242125 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Notes
IHC-P
1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IHC-Fr
1/100.

Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

Application notes
Is unsuitable for IP or WB.

Target

  • Function

    Transcriptional activator.
  • Tissue specificity

    Mainly expressed in the developing central nervous system.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Target information above from: UniProt accession O00570 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 20664 Mouse
    • SwissProt: P53783 Mouse
    • Unigene: 39088 Mouse
    • Alternative names

      • Sex determining region Y box 1 antibody
      • sox1 antibody
      • SOX1_HUMAN antibody
      • SRY antibody
      • SRY box containing gene 1 antibody
      • SRY related HMG box gene 1 antibody
      • Transcription factor Sox-1 antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse cerebrum tissue labelling NeuN with ab177487 at 1/100 dilution (B), SOX1 with ab242125 at 1/100 dilution (C) and Olig2 with ab109186 at 1/100 dilution (D). Anti-Rabbit and Mouse Polymer HRP was used as a secondary antibody, and DAPI was used for a nuclear counter stain. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins. Heat mediated antigen retrieval (Leica ER2, PH9.0, 20 minutes) was used in between rounds of tyramide signal amplification to remove the antibodies from the previous round, to avoid any cross-reactivity. 

      Panel A: merged staining of anti- NeuN (green, Opal™520), anti-SOX1 (red, Opal™570) and anti- Olig2 (yellow, Opal™690).

      Panel B: anti-NeuN stands for neurons.

      Panel C: anti-SOX1 stained on neural progenitors.

      Panel D: anti-Olig2 stained on oligodendrocyte.

      The section was incubated in three rounds of staining: in the order of ab177487, ab242125 and ab109186 for 30 mins at room temperature. Each round was followed by a separate fluorescent tyramide signal amplification system.

      The immunostaining was performed on a Leica Biosystems BOND® RX instrument with an Opal™ 4-color kit. Image acquisition was performed with Leica SP8 confocal microscope.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on neural progenitor cells in rat cerebellum is observed. The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized rat cerebellum tissue labeling SOX1 with ab242125 at 1/100 followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on rat cerebellum (PMID: 24676388) is observed. The nuclear counterstain is DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemical analysis of paraffin-embedded mouse testis tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Negative control: No staining on the mouse testis.

      The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemical analysis of paraffin-embedded mouse hippocampus tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on neural progenitor cells in mouse hippocampus is observed (PMID: 22992951). The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Frozen sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemical analysis of frozen section of 4% PFA-fixed, 0.2% Triton X-100 permeabilized mouse cerebellum tissue labeling SOX1 with ab242125 at 1/100 followed by ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution (Green). Nuclear staining on mouse cerebellum (PMID: 24676388) is observed. The nuclear counterstain is DAPI (Blue).

      Secondary antibody only control: Secondary antibody is ab150077 AlexaFluor®488 Goat anti-Rabbit secondary at 1/1000 dilution.

      Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR23041-60] (ab242125)

      Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue labeling SOX1 with ab242125 at 1/2000 dilution (0.293 µg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on neural progenitor cells in mouse cerebellum is observed (PMID: 24676388). The section was incubated with ab242125 for 15 mins at room temperature. The immunostaining staining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with hematoxylin.

      Secondary antibody only control: Secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

      Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 mins.

    • Anti-SOX1 antibody [EPR23041-60] (ab242125)
      Anti-SOX1 antibody [EPR23041-60] (ab242125)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    Certificate of Compliance

    To download a Certificate of Compliance, please enter your Lot number below:

    References (0)

    Publishing research using ab242125? Please let us know so that we can cite the reference in this datasheet.

    ab242125 has not yet been referenced specifically in any publications.

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