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Rabbit monoclonal [EPR4766] to SOX1
ICC/IF more details Unsuitable for:
Synthetic peptide within Human SOX1. The exact sequence is proprietary.
NCCIT, SH-SY5Y, MCF7, and HeLa cell lysates, Human fetal brain tissue, NCCIT cells
® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab ® patents
This product is a recombinant rabbit monoclonal antibody.
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Preservative: 0.05% Sodium azide Constituents: 0.1% BSA, 40% Glycerol, 9.85% Tris glycine, 50% Tissue culture supernatant
Tissue culture supernatant
Abpromise guarantee covers the use of
in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
1/1000 - 1/10000. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
1/100 - 1/250. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Use at an assay dependent concentration.
ab172730 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody.
1/100 - 1/250.
Is unsuitable for IP.
Mainly expressed in the developing central nervous system.
Contains 1 HMG box DNA-binding domain.
Information by UniProt
Sex determining region Y box 1 antibody
Western blot - Anti-SOX1 antibody [EPR4766] (ab109290)
All lanes : Anti-SOX1 antibody [EPR4766] (ab109290) at 1/1000 dilution Lane 1 : NCCIT cell lysate Lane 2 : SH-SY5Y cell lysate Lane 3 : MCF7 cell lysate Lane 4 : HeLa cell lysate Lysates/proteins at 10 µg per lane. Predicted band size: 39 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR4766] (ab109290)
Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue using ab109290 at a dilution of 1/100.
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] (ab109290)
Immunofluorescent staining of NCCIT cells using ab109290 at a dilution of 1/100.
Flow Cytometry - Anti-SOX1 antibody [EPR4766] (ab109290)
Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX1 with unpurified ab109290 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This product has been referenced in:
et al. Epigenetic regulation of human SOX3 gene expression during early phases of neural differentiation of NT2/D1 cells. PLoS One 12:e0184099 (2017).
Read more (PubMed: 28886103) »
et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014).
Read more (PubMed: 24637840) »
See all 4 Publications
for this product
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"