RecombinantRabMAb

Recombinant Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

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Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4766] to SOX1 - BSA and Azide free
  • Suitable for: Flow Cyt, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Human

Overview

  • Product name

    Anti-SOX1 antibody [EPR4766] - BSA and Azide free
    See all SOX1 primary antibodies

  • Description

    Rabbit monoclonal [EPR4766] to SOX1 - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
    Unsuitable for: IP

  • Species reactivity

    Reacts with: Mouse, Human

  • Immunogen

    Synthetic peptide corresponding to residues in Human SOX1.

  • Positive control

    • NCCIT, SH-SY5Y, MCF7, and HeLa cell lysates, Human fetal brain tissue, NCCIT cells

  • General notes

    Ab227118 is the carrier-free version of ab109290. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab227118 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab227118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.

 
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
ICC/IF Use at an assay dependent concentration.
  • Application notes
    Is unsuitable for IP.

    • Target

    Images

    • Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

      This ICC/IF data was generated using the same anti-SOX1 antibody clone, EPR4766, in a different buffer formulation (cat# ab109290).

      Immunofluorescent staining of NCCIT cells using ab109290 at a dilution of 1/100.

    • Flow Cytometry - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
      Flow Cytometry - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

      Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX1 with unpurified ab109290 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109290).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

      Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue using ab109290 at a dilution of 1/100.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109290).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
      Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (3)

    Publishing research using ab227118? Please let us know so that we can cite the reference in this datasheet.

    ab227118 has been referenced in 3 publications.

    • Wang H  et al. Genetic screening and multipotency in rhesus monkey haploid neural progenitor cells. Development 145:N/A (2018). PubMed: 29784672
    • Popovic J  et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB ; Human . PubMed: 24637840
    • Ko HA  et al. Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum. Neurosci Lett 548:44-9 (2013). ICC/IF ; Mouse . PubMed: 23684982

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