Recombinant Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)
- Datasheet
- References (2)
- Protocols
Overview
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Product name
Anti-SOX1 antibody [EPR4766] - BSA and Azide free
See all SOX1 primary antibodies -
Description
Rabbit monoclonal [EPR4766] to SOX1 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
Unsuitable for: IP -
Species reactivity
Reacts with: Mouse, Human -
Immunogen
Synthetic peptide corresponding to residues in Human SOX1.
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Positive control
- NCCIT, SH-SY5Y, MCF7, and HeLa cell lysates, Human fetal brain tissue, NCCIT cells
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General notes
Ab227118 is the carrier-free version of ab109290. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.
Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
ab227118 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.
Maxpar® is a trademark of Fluidigm Canada Inc.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. -
Storage buffer
pH: 7.20
Constituent: PBS -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4766 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Positive Controls
Applications
Our Abpromise guarantee covers the use of ab227118 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use at an assay dependent concentration. ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
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| IHC-P | Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. | |
| WB | Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa). | |
| ICC/IF | Use at an assay dependent concentration. |
Target
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Function
Transcriptional activator. -
Tissue specificity
Mainly expressed in the developing central nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
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Alternative names
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
see all
Images
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![Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)](https://www.abcam.com/ps/products/227/ab227118/Images/ab227118-288843-ab109290ICC.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118) -
![Flow Cytometry - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)](https://www.abcam.com/ps/products/227/ab227118/Images/ab227118-3-ab109290269689ab109290FC1.jpg)
Flow Cytometry - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX1 with unpurified ab109290 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109290).
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)](https://www.abcam.com/ps/products/227/ab227118/Images/ab227118-2-SOX1Primaryantibodiesab1092905.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue using ab109290 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109290).
Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
Datasheets and documents
References
This product has been referenced in:
- Popovic J et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB ; Human . Read more (PubMed: 24637840) »
- Ko HA et al. Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum. Neurosci Lett 548:44-9 (2013). ICC/IF ; Mouse . Read more (PubMed: 23684982) »
