Anti-SOX1 antibody [EPR4766] - BSA and Azide free Recombinant (ab227118)

Product name

Anti-SOX1 antibody [EPR4766] - BSA and Azide free
See all SOX1 primary antibodies
Description

Rabbit monoclonal [EPR4766] to SOX1 - BSA and Azide free
Host species

Rabbit
Tested applications

Suitable for: Flow Cyt, IHC-P, WB, ICC/IFmore details
Unsuitable for: IP
Species reactivity

Reacts with: Mouse, Human
Immunogen

Synthetic peptide corresponding to residues in Human SOX1.
Positive control
- NCCIT, SH-SY5Y, MCF7, and HeLa cell lysates, Human fetal brain tissue, NCCIT cells
General notes

ab227118 is a PBS only buffer version of ab109290, containing no BSA or sodium azide, ideal for antibody labeling. Please refer to ab109290 for information on protocols, dilutions, and image data.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
Storage buffer

pH: 7.20
Constituent: PBS
Concentration information loading...
Purity

Affinity purified
Clonality

Monoclonal
Clone number

EPR4766
Isotype

IgG
Research areas

Our Abpromise guarantee covers the use of ab227118 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
Flow Cyt		Use at an assay dependent concentration. ab199376 - Rabbit monoclonal (Low endotoxin, Azide free), is suitable for use as an isotype control with this antibody.
IHC-P		Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
WB		Use at an assay dependent concentration. Detects a band of approximately 40 kDa (predicted molecular weight: 39 kDa).
ICC/IF		Use at an assay dependent concentration.

Application notes

Is unsuitable for IP.

Function

Transcriptional activator.
Tissue specificity

Mainly expressed in the developing central nervous system.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Cellular localization

Nucleus.
Target information above from: UniProt accession O00570 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 6656 Human
- Entrez Gene: 20664 Mouse
- Omim: 602148 Human
- SwissProt: O00570 Human
- SwissProt: P53783 Mouse
- Unigene: 202526 Human
- Unigene: 39088 Mouse
Alternative names
- Sex determining region Y box 1 antibody
- sox1 antibody
- SOX1_HUMAN antibody
- SRY antibody
- SRY box containing gene 1 antibody
- SRY related HMG box gene 1 antibody
- Transcription factor Sox-1 antibody
see all

Flow Cytometry - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

Flow Cytometry analysis of NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX1 with unpurified ab109290 at 1/100 dilution (10ug/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black) was used as the isotype control, cells without incubation with primary antibody and secondary antibody (Blue) was used as the unlabeled control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109290).
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue using ab109290 at a dilution of 1/100.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab109290).
Immunocytochemistry/ Immunofluorescence - Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)

This ICC/IF data was generated using the same anti-SOX1 antibody clone, EPR4766, in a different buffer formulation (cat# ab109290).

Immunofluorescent staining of NCCIT cells using ab109290 at a dilution of 1/100.

Popovic J et al. Expression analysis of SOX14 during retinoic acid induced neural differentiation of embryonal carcinoma cells and assessment of the effect of its ectopic expression on SOXB members in HeLa cells. PLoS One 9:e91852 (2014). WB ; Human . Read more (PubMed: 24637840) »
Ko HA et al. Cell type-selective expression of the zinc finger-containing gene Nolz-1/Zfp503 in the developing mouse striatum. Neurosci Lett 548:44-9 (2013). ICC/IF ; Mouse . Read more (PubMed: 23684982) »

Publishing research using ab227118? Please let us know so that we can cite the reference in this datasheet.

Anti-SOX1 antibody [EPR4766] - BSA and Azide free (ab227118)