Key features and details
- Mouse monoclonal [2E7B5] to SOX10
- Suitable for: WB, Flow Cyt
- Reacts with: Human
- Isotype: IgG1
Product nameAnti-SOX10 antibody [2E7B5]
See all SOX10 primary antibodies
DescriptionMouse monoclonal [2E7B5] to SOX10
Tested applicationsSuitable for: WB, Flow Cytmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Pig
- SOX10 recombinant protein; SOX10 (Amino acid: 147-252)-hIgGFc transfected HEK293 cell lysate; HepG2 cells.
This product was changed from ascites to supernatant. Lot no’s high than GR243375-14 are from Tissue Culture Supernatant
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.05% Sodium azide
Constituent: 99% PBS
Contains 0.5% protein stabilizer.
Concentration information loading...
PurityProtein G purified
Purification notesPurified from tissue culture supernatant.
Our Abpromise guarantee covers the use of ab181466 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/2000. Predicted molecular weight: 49 kDa.|
|Flow Cyt||1/200 - 1/400.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
FunctionTranscription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
Tissue specificityExpressed in fetal brain and in adult brain, heart, small intestine and colon.
Involvement in diseaseDefects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
Flow cytometric analysis of HepG2 cells labeling SOX10 with ab181466 at 1/200 dilution (green) compared to a negative control (red).
All lanes : Anti-SOX10 antibody [2E7B5] (ab181466) at 1/500 dilution
Lane 1 : HEK293 cell lysate.
Lane 2 : SOX10 (Amino acids: 147-252)-hIgGFc transfected HEK293 cell lysate.
Predicted band size: 49 kDa
Anti-SOX10 antibody [2E7B5] (ab181466) at 1/500 dilution + SOX10 recombinant protein
Predicted band size: 49 kDa
Expected MWt is 31.7 kDa.
ab181466 has been referenced in 1 publication.
- Vazquez SE et al. Identification of novel, clinically correlated autoantigens in the monogenic autoimmune syndrome APS1 by proteome-wide PhIP-Seq. Elife 9:N/A (2020). PubMed: 32410729