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    sox10-antibody-ab221733.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-SOX10 antibody (ab221733)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab221733)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody (ab221733)

Key features and details

  • Rabbit polyclonal to SOX10
  • Suitable for: IHC-P, ICC/IF
  • Reacts with: Human
  • Isotype: IgG

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Overview

  • Product name

    Anti-SOX10 antibody
    See all SOX10 primary antibodies
  • Description

    Rabbit polyclonal to SOX10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Chicken, Pig
  • Immunogen

    Recombinant fragment corresponding to Human SOX10 aa 367-416.
    Sequence:

    PHYTDQPSTSQIAYTSLSLPHYGSAFPSISRPQFDYSDHQPSGPYYGHSG


    Database link: P56693
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI
  • Positive control

    • IHC-P: Human cerebral cortex tissue. ICC/IF: SK-MEL-30 cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.02% Sodium azide
    Constituents: PBS, 40% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SOX10 protein (ab114238)
  • Related Products

    • Recombinant Human SOX10 protein (ab114238)

Applications

Our Abpromise guarantee covers the use of ab221733 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/500 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.25 - 2 µg/ml.

Fixation/Permeabilization: PFA/Triton X-100.

Target

  • Function

    Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
  • Tissue specificity

    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • Involvement in disease

    Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
    Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
    Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P56693 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395573 Chicken
    • Entrez Gene: 6663 Human
    • Entrez Gene: 20665 Mouse
    • Entrez Gene: 414903 Pig
    • Entrez Gene: 29361 Rat
    • Omim: 602229 Human
    • SwissProt: Q9W757 Chicken
    • SwissProt: P56693 Human
    • SwissProt: Q04888 Mouse
    • SwissProt: A5A763 Pig
    • SwissProt: O55170 Rat
    • Unigene: 376984 Human
    • Unigene: 276739 Mouse
    • Unigene: 10883 Rat
    see all
  • Alternative names

    • DOM antibody
    • DOM antibody
    • Dominant megacolon mouse human homolog of antibody
    • MGC15649 antibody
    • PCWH antibody
    • SOX 10 antibody
    • SOX10 antibody
    • SOX10_HUMAN antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY box 10 antibody
    • SRY box containing gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • Transcription factor SOX 10 antibody
    • Transcription factor SOX-10 antibody
    • WS2E antibody
    • WS4 antibody
    • WS4C antibody
    see all

Images

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab221733)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab221733)

    Paraffin-embedded human cerebral cortex tissue stained for SOX10 using ab221733 at 1/500 dilution in Immunohistochemical analysis.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody (ab221733)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody (ab221733)

    PFA-fixed, Triton X-100 permeabilized SK-MEL-30 cells stained for SOX10  (green) using ab221733 at 4 µg/ml in ICC/IF.

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
  • References (1)

    Publishing research using ab221733? Please let us know so that we can cite the reference in this datasheet.

    ab221733 has been referenced in 1 publication.

    • Wu M  et al. A large-scale collection of giant congenital melanocytic nevi: Clinical and histopathological characteristics. Exp Ther Med 19:313-318 (2020). PubMed: 31853305

    Customer reviews and Q&As

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