Anti-SOX10 antibody (ab27655)
- Datasheet
- References (10)
- Protocols
Overview
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Product name
Anti-SOX10 antibody
See all SOX10 primary antibodies -
Description
Rabbit polyclonal to SOX10 -
Host species
Rabbit -
Specificity
Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9 -
Tested applications
Suitable for: ICC/IF, IHC-Fr, WBmore details -
Species reactivity
Reacts with: Mouse, Chicken -
Immunogen
Synthetic peptide:
CPPAHSPTANWDQPVYTTLT
, corresponding to C terminal amino acids 449-468 of Chicken SOX8
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading... -
Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab27655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | 1/1000 - 1/5000. | |
| IHC-Fr | 1/5000. Fix with 4% paraformaldehyde for 2h at 4C. | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. -
Tissue specificity
Expressed in fetal brain and in adult brain, heart, small intestine and colon. -
Involvement in disease
Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395573 Chicken
- Entrez Gene: 20665 Mouse
- SwissProt: Q9W757 Chicken
- SwissProt: Q04888 Mouse
- Unigene: 276739 Mouse
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Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab27655)Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion. -
Western blot - Anti-SOX10 antibody (ab27655)All lanes : Anti-SOX10 antibody (ab27655) at 1/500 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Datasheets and documents
References
This product has been referenced in:
- Hu B et al. Myelin abnormality in Charcot-Marie-Tooth type 4J recapitulates features of acquired demyelination. Ann Neurol 83:756-770 (2018). Read more (PubMed: 29518270) »
- Pelletier J et al. Generation and characterization of polyclonal and monoclonal antibodies to human NTPDase2 including a blocking antibody. Purinergic Signal 13:293-304 (2017). Read more (PubMed: 28409324) »
