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    sox10-antibody-ab27655.pdf

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Neuroscience Cell Type Marker Neural Stem Cell marker
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Anti-SOX10 antibody (ab27655)

  • Datasheet
Reviews (5)Q&A (11)References (11)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab27655)
  • Western blot - Anti-SOX10 antibody (ab27655)

Key features and details

  • Rabbit polyclonal to SOX10
  • Suitable for: IHC-P, WB
  • Reacts with: Mouse, Chicken
  • Isotype: IgG

You may also be interested in

Protein
Product image
Recombinant Human SOX10 protein (ab114238)
Secondary
Product image
Goat Anti-Rabbit IgG H&L (HRP) (ab205718)

View more associated products

Overview

  • Product name

    Anti-SOX10 antibody
    See all SOX10 primary antibodies
  • Description

    Rabbit polyclonal to SOX10
  • Host species

    Rabbit
  • Specificity

    Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9
  • Tested Applications & Species

    Application Species
    IHC-P
    Chicken
    WB
    Mouse
    See all applications and species data
  • Immunogen

    Synthetic peptide:

    CPPAHSPTANWDQPVYTTLT

    , corresponding to C terminal amino acids 449-468 of Chicken SOX8
    Run BLAST with BLAST the sequence with ExPASy Run BLAST with BLAST the sequence with NCBI

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Concentration information loading...
  • Purity

    Whole antiserum
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human SOX10 protein (ab114238)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab27655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Guaranteed

Tested applications are guaranteed to work and covered by our Abpromise guarantee.

Predicted

Predicted to work for this combination of applications and species but not guaranteed.

Incompatible

Does not work for this combination of applications and species.

Application Species
IHC-P
Chicken
WB
Mouse
Application Abreviews Notes
IHC-P
Use at an assay dependent concentration.
WB (2)
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
Notes
IHC-P
Use at an assay dependent concentration.
WB
Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).

Target

  • Function

    Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
  • Tissue specificity

    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • Involvement in disease

    Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
    Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
    Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P56693 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395573 Chicken
    • Entrez Gene: 20665 Mouse
    • SwissProt: Q9W757 Chicken
    • SwissProt: Q04888 Mouse
    • Unigene: 276739 Mouse
    • Alternative names

      • DOM antibody
      • DOM antibody
      • Dominant megacolon mouse human homolog of antibody
      • MGC15649 antibody
      • PCWH antibody
      • SOX 10 antibody
      • SOX10 antibody
      • SOX10_HUMAN antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY box 10 antibody
      • SRY box containing gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • Transcription factor SOX 10 antibody
      • Transcription factor SOX-10 antibody
      • WS2E antibody
      • WS4 antibody
      • WS4C antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab27655)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody (ab27655)
      Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion.
    • Western blot - Anti-SOX10 antibody (ab27655)
      Western blot - Anti-SOX10 antibody (ab27655)
      All lanes : Anti-SOX10 antibody (ab27655) at 1/500 dilution

      Lane 1 : Brain (Mouse) Tissue Lysate
      Lane 2 : Heart (Mouse) Tissue Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 50 kDa
      Observed band size: 56 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 8 minutes


      SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (11)

    Publishing research using ab27655? Please let us know so that we can cite the reference in this datasheet.

    ab27655 has been referenced in 11 publications.

    • Hu B  et al. Myelin abnormality in Charcot-Marie-Tooth type 4J recapitulates features of acquired demyelination. Ann Neurol 83:756-770 (2018). PubMed: 29518270
    • Falcone C  et al. Cortical interlaminar astrocytes across the therian mammal radiation. J Comp Neurol N/A:N/A (2018). PubMed: 30552685
    • Pelletier J  et al. Generation and characterization of polyclonal and monoclonal antibodies to human NTPDase2 including a blocking antibody. Purinergic Signal 13:293-304 (2017). PubMed: 28409324
    • Chen Y  et al. GSK3ß inhibition accelerates axon debris clearance and new axon remyelination. Am J Transl Res 8:5410-5420 (2016). PubMed: 28078012
    • Werner A  et al. Cell-fate determination by ubiquitin-dependent regulation of translation. Nature 525:523-7 (2015). PubMed: 26399832
    View all Publications for this product

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    1-10 of 16 Abreviews or Q&A

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX10 antibody

    Average
    Abreviews
    Abreviews
    abreview image
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Monkey Cell (Kidney)
    Permeabilization
    Yes - 0.1% triton x-100
    Specification
    Kidney
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jul 01 2016

    Western blot abreview for Anti-SOX10 antibody

    Below Average
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Monkey Cell lysate - whole cell (Kiney)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Kiney
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2016

    Western blot abreview for Anti-SOX10 antibody

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Western blot
    Sample
    Mouse Tissue lysate - whole (Brain)
    Gel Running Conditions
    Non-reduced Non-Denaturing (Native)
    Loading amount
    30 µg
    Specification
    Brain
    Blocking step
    BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 02 2016

    Immunohistochemistry (Frozen sections) abreview for Anti-SOX10 antibody

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Frozen sections)
    Sample
    Mouse Tissue sections (Brain)
    Permeabilization
    Yes - 0.3% triton x-100
    Specification
    Brain
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Jun 01 2016

    Question

    We have some doubts about the data sheat of this ab. For the specificity it is written that: "Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9"
    Can you clarify better what you mean? Is it correct or there is a spelling error? Does it mean that the ab recognise both Sox10 and Sox8 or is it really specific for Sox10?

    Read More

    Abcam community

    Verified customer

    Asked on Jul 12 2013

    Answer



    The immunogen was actually chicken Sox8. However, we are confident that it also recognizes Sox10 and does not cross react with Sox9.

    The originator used what turns out to be the chick Sox8 sequence for the peptide. (When they originally designed the peptide the identification of chick Sox8 and Sox10 wasn't 100% clear). However, from characterization of the antibody it definitely recognizes Sox10 in both mouse and chick tissue, this is not too surprising as the genes are highly related. It doesn't cross react, at least in assays in either chick or mouse, with Sox9 - another SoxE family member. It probably also recognizes Sox8 however we're not aware of any tissue that expresses Sox8 in the absence of Sox10, so its not possible to test this directly.

    Read More

    Elisa Thomas

    Abcam Scientific Support

    Answered on Jul 12 2013

    Question

    Bonjour,
    Merci de votre réponse.
    Nous serions éventuellement intéressé par un autre lot de ab2765 s’il provient d’un lapin dont le sérum vous a permis d’obtenir le marquage que vous présentez dans la fiche technique.
    Par contre si les lots que vous avez en stock n’ont pas été testé en Immunohistochimie par votre service qualité nous préférions bénéficier d’un avoir.
    Je reste à votre disposition si vous avez besoin d’informations complémentaires. J’ai notamment oublié de vous préciser lors de mon précédent mail que l’anticorps avait bien été aliquoté et conservé à -20°C après sa réception.

    Read More

    Abcam community

    Verified customer

    Asked on Nov 30 2012

    Answer

    Merci pour votre email.

    J'ai mis en place l'envoi d'une nouvelle unité de ab27655, le numéro de commande de remplacement gratuit de ce produit est ***.
    Par mesure préventive, j'ai pris l'initiative de retarder l'envoi du ab72876 à lundi afin qu'il ne reste pas en transit tout le weekend. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

    Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

    Read More

    Abcam Scientific Support

    Answered on Nov 30 2012

    Question

    Product code: 27655
    Lot number: GR93638-1

    Inquiry:
    Hello,
    We purchased your anti-Sox-10 antibody (ab27655) to label neural cell crest on cryostat section of chick embryos (E3.5 to E4.5).
    Unfortunately, we were not able to get the nice labelling that is shown on the datasheet.
    We tried it on embryos fixed in 4% PFA for either 2h or overnight at 4°c, with different permeabilisation (triton 0,5% or 0,1%), blocking solutions (horse serum or BSA) and antibody concentrations (1/1000 to 1/100).
    The secondary antibody was an anti-rabbit Alexa 555 1/400 and the washes were performed in PBS 1X (two washes of 20 minutes after the primary and secondary antibody).
    Although, the labelling seems better with a 1h30 pre-blocking/premeabilisation in BSA 6% + 0,5% triton and an overnight incubation of the antibody diluted in BSA 1% +/- 0.1%Triton, it is still really weak.
    Thus, I would like to know if you have any suggestions to improve you IHC or if you could provide us some more detailed description of the procedure used to get the labelling you show?
    Thanks

    Read More

    Abcam community

    Verified customer

    Asked on Nov 28 2012

    Answer

    Thank you for contacting us.

    I am sorry to hear this antibody is not providing satisfactory results.

    The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

    Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

    I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.
    If a replacement is a better resolution for you, I can offer:

    one unit of a different lot of ab27655
    one unit of anti-SOX10 ab107532 which shows 87% homology with the chicken protein, www.abcam.com/ab107532
    one unit of anti-SOX10 ab64055 which also shows 87% homology with the chicken protein, www.abcam.com/ab64055




    Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed. Could you also please confirm the name and address of your lab?

    Read More

    Abcam Scientific Support

    Answered on Nov 28 2012

    Immunocytochemistry/ Immunofluorescence abreview for Anti-SOX10 antibody

    Excellent
    Abreviews
    Abreviews
    Application
    Immunocytochemistry/ Immunofluorescence
    Sample
    Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
    Specification
    Dorsal Root Ganglia (DRG) neurons
    Fixative
    Paraformaldehyde
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C
    Read More

    Abcam user community

    Verified customer

    Submitted Oct 18 2012

    Question

    Hello, A collegue contact you on July 30th for a problem with antibody which stay at room temperature during a hot week end. I tested this antibody on spinal columns of P0 mouse with the same protocol published by Mukhopadhyay A et al. HeyL regulates the number of TrkC neurons in dorsal root ganglia. Dev Biol 334:142-51 (2009). IHC-Fr Mouse. I tested, also, several dilution for this antibody. Unfortunately, the antibody does not work. Can you replace it ? Sincerely yours,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 24 2012

    Answer

    Thank you for contacting us.

    I am very sorry for the inconveniences and am happy to create a free of charge replacement order in compensation for the problems experienced with this antibody. The order number is XXXXXX. You should receive an automatic confirmation. To check the status of the order please contact our Customer Service team and reference this number.

    I hope this new vial works as expected. Please do not hesitate to contact us if you need any more advice or information.

    Read More

    Abcam Scientific Support

    Answered on Aug 24 2012

    Question

    Dear xxxxx,
    thanks for your support. I will be looking forward for any input. Besides your two antibodies we also tested the xxxx from xxxxxx and the xxxxxx from xxxxxx. Both with negative results in adult PNS tissue. In terms of Schwann cell markers we have functional S100beta antibody, but the point is that we need a nuclear Schwann cell marker to colocalize with BrdU and Tunel-positive cells.
    We therefore would prefer a voucher for an antibody of yours at a point we would need one, if possible. If that is not possible I could check which antibody of yours we successfully use.
    Best wishes,

    Read More

    Abcam community

    Verified customer

    Asked on Aug 01 2012

    Answer

    Thank you for your reply.

    I can understand your situation. I cannot myself explain why all these antibodies would not be detecting the SOX10 protein in your samples. I will have my colleague look into this when she is back next week and hopefully she can provide some input.

    I can certainly arrange for a credit note to be issued to you. This can be used to against the purchase of any antibody or product from our catalogue. Or if its easier for you, I can arrange for a refund or to provide you with any primary antibody of your choice from our catalogue free of charge. Please do let me know how you would like to proceed.

    I look forward to receiving your reply.

    Read More

    Abcam Scientific Support

    Answered on Aug 01 2012

    1-10 of 16 Abreviews or Q&A

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