Overview

  • Product name
  • Description
    Rabbit polyclonal to SOX10
  • Host species
    Rabbit
  • Specificity
    Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9
  • Tested applications
    Suitable for: ICC/IF, IHC-Fr, WBmore details
  • Species reactivity
    Reacts with: Mouse, Chicken
  • Immunogen

    Synthetic peptide:

    CPPAHSPTANWDQPVYTTLT

    , corresponding to C terminal amino acids 449-468 of Chicken SOX8

Properties

Applications

Our Abpromise guarantee covers the use of ab27655 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/1000 - 1/5000.
IHC-Fr 1/5000. Fix with 4% paraformaldehyde for 2h at 4C.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).

Target

  • Function
    Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
  • Tissue specificity
    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • Involvement in disease
    Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
    Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
    Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • DOM antibody
    • DOM antibody
    • Dominant megacolon mouse human homolog of antibody
    • MGC15649 antibody
    • PCWH antibody
    • SOX 10 antibody
    • SOX10 antibody
    • SOX10_HUMAN antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY box 10 antibody
    • SRY box containing gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • Transcription factor SOX 10 antibody
    • Transcription factor SOX-10 antibody
    • WS2E antibody
    • WS4 antibody
    • WS4C antibody
    see all

Images

  • Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion.
  • All lanes : Anti-SOX10 antibody (ab27655) at 1/500 dilution

    Lane 1 : Brain (Mouse) Tissue Lysate
    Lane 2 : Heart (Mouse) Tissue Lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 50 kDa
    Observed band size: 56 kDa
    why is the actual band size different from the predicted?
    Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.


    Exposure time: 8 minutes


    SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

References

This product has been referenced in:
  • Pelletier J  et al. Generation and characterization of polyclonal and monoclonal antibodies to human NTPDase2 including a blocking antibody. Purinergic Signal 13:293-304 (2017). Read more (PubMed: 28409324) »
  • Chen Y  et al. GSK3ß inhibition accelerates axon debris clearance and new axon remyelination. Am J Transl Res 8:5410-5420 (2016). Read more (PubMed: 28078012) »
See all 9 Publications for this product

Customer reviews and Q&As

1-10 of 16 Abreviews or Q&A

Abcam has not validated the combination of species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Monkey Cell (Kidney)
Permeabilization
Yes - 0.1% triton x-100
Specification
Kidney
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 01 2016

Abreviews
Application
Western blot
Sample
Monkey Cell lysate - whole cell (Kiney)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Kiney
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2016

Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Non-Denaturing (Native)
Loading amount
30 µg
Specification
Brain
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jun 02 2016

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Brain)
Permeabilization
Yes - 0.3% triton x-100
Specification
Brain
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 37°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jun 01 2016

Answer



The immunogen was actually chicken Sox8. However, we are confident that it also recognizes Sox10 and does not cross react with Sox9.

The originator used what turns out to be the chick Sox8 sequence for the peptide. (When they originally designed the peptide the identification of chick Sox8 and Sox10 wasn't 100% clear). However, from characterization of the antibody it definitely recognizes Sox10 in both mouse and chick tissue, this is not too surprising as the genes are highly related. It doesn't cross react, at least in assays in either chick or mouse, with Sox9 - another SoxE family member. It probably also recognizes Sox8 however we're not aware of any tissue that expresses Sox8 in the absence of Sox10, so its not possible to test this directly.

Read More

Answer

Merci pour votre email.

J'ai mis en place l'envoi d'une nouvelle unité de ab27655, le numéro de commande de remplacement gratuit de ce produit est ***.
Par mesure préventive, j'ai pris l'initiative de retarder l'envoi du ab72876 à lundi afin qu'il ne reste pas en transit tout le weekend. Vous recevrez prochainement un mail de confirmation comprenant les détails d'expédition.

Veuillez noter que cet anticorps de remplacement est également couvert par la garantie Abpromise, donc n'hésitez pas à nous contacter de nouveau si nécessaire.

Read More

Answer

Thank you for contacting us.

I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. I appreciate the time you have spent in the laboratory and understand your concerns. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results. It appears that you may have received a faulty vial.

I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation.
If a replacement is a better resolution for you, I can offer:

one unit of a different lot of ab27655
one unit of anti-SOX10 ab107532 which shows 87% homology with the chicken protein, www.abcam.com/ab107532
one unit of anti-SOX10 ab64055 which also shows 87% homology with the chicken protein, www.abcam.com/ab64055




Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed. Could you also please confirm the name and address of your lab?

Read More
Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Dorsal Root Ganglia (DRG) neurons)
Specification
Dorsal Root Ganglia (DRG) neurons
Fixative
Paraformaldehyde
Blocking step
Serum as blocking agent for 30 minute(s) · Concentration: 2% · Temperature: 21°C

Abcam user community

Verified customer

Submitted Oct 18 2012

Answer

Thank you for contacting us.

I am very sorry for the inconveniences and am happy to create a free of charge replacement order in compensation for the problems experienced with this antibody. The order number is XXXXXX. You should receive an automatic confirmation. To check the status of the order please contact our Customer Service team and reference this number.

I hope this new vial works as expected. Please do not hesitate to contact us if you need any more advice or information.

Read More

Answer

Thank you for your reply.

I can understand your situation. I cannot myself explain why all these antibodies would not be detecting the SOX10 protein in your samples. I will have my colleague look into this when she is back next week and hopefully she can provide some input.

I can certainly arrange for a credit note to be issued to you. This can be used to against the purchase of any antibody or product from our catalogue. Or if its easier for you, I can arrange for a refund or to provide you with any primary antibody of your choice from our catalogue free of charge. Please do let me know how you would like to proceed.

I look forward to receiving your reply.

Read More

1-10 of 16 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Sign up