Anti-SOX8 antibody (ab27655)
Key features and details
- Rabbit polyclonal to SOX8
- Suitable for: IHC-P, WB
- Reacts with: Mouse, Chicken
- Isotype: IgG
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Overview
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Product name
Anti-SOX8 antibody
See all SOX8 primary antibodies -
Description
Rabbit polyclonal to SOX8 -
Host species
Rabbit -
Specificity
Antibody was raised using a synthetic peptide corresponding to Sox8. However, this antibody demonstrates cross reactivity against Sox10. Does not cross react with SOX9 -
Tested applications
Suitable for: IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Chicken -
Immunogen
Synthetic peptide corresponding to Chicken SOX8 aa 400-500 (C terminal).
Database link: P57074 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Concentration information loading...
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Purity
Whole antiserum -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab27655 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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IHC-P |
Use at an assay dependent concentration.
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WB | (2) |
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa).
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Notes |
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IHC-P
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 56 kDa (predicted molecular weight: 50 kDa). |
Target
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Function
May play a role in central nervous system, limb and facial development. May be involved in male sex determination. Binds the consensus motif 5'-[AT][AT]CAA[AT]G-3'. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395483 Chicken
- Entrez Gene: 20681 Mouse
- SwissProt: P57074 Chicken
- SwissProt: Q04886 Mouse
- Unigene: 258220 Mouse
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Alternative names
- MGC24837 antibody
- SOX 8 antibody
- Sox8 antibody
see all
Images
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Sections through the trunk of E4 chick embryos fixed in 4 % paraformaldehyde and stained with anti-SOX10 (ab27655) diluted 1/5000. Secondary antibody was FITC-conjugated donkey anti-rabbit. ab27655 stains the embryonic neural crest cells which can be seen migrating into the dorsal root ganglion.
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All lanes : Anti-SOX8 antibody (ab27655) at 1/500 dilution
Lane 1 : Brain (Mouse) Tissue Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 50 kDa
Observed band size: 56 kDa why is the actual band size different from the predicted?
Additional bands at: 100 kDa, 74 kDa. We are unsure as to the identity of these extra bands.
Exposure time: 8 minutes
SOX10 contains a number of potential phosphorylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
References (17)
ab27655 has been referenced in 17 publications.
- Mac Donald K & Iulianella A The actin-cytoskeleton associating protein BASP1 regulates neural progenitor localization in the neural tube. Genesis 60:e23464 (2022). PubMed: 34897971
- Inubushi T et al. The cell surface hyaluronidase TMEM2 plays an essential role in mouse neural crest cell development and survival. PLoS Genet 18:e1009765 (2022). PubMed: 35839257
- Zhang S et al. HIFa Regulates Developmental Myelination Independent of Autocrine Wnt Signaling. J Neurosci 41:251-268 (2021). PubMed: 33208471
- Zhang S et al. The Wnt Effector TCF7l2 Promotes Oligodendroglial Differentiation by Repressing Autocrine BMP4-Mediated Signaling. J Neurosci 41:1650-1664 (2021). PubMed: 33452226
- Huang T et al. Optogenetically Controlled TrkA Activity Improves the Regenerative Capacity of Hair-Follicle-Derived Stem Cells to Differentiate into Neurons and Glia. Adv Biol (Weinh) 5:e2000134 (2021). PubMed: 32924336