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    sox10-antibody-epr4007-104-ab180862.pdf

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RecombinantRabMAb

Recombinant Anti-SOX10 antibody [EPR4007-104] (ab180862)

  • Datasheet
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
  • Anti-SOX10 antibody [EPR4007-104] (ab180862)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR4007-104] to SOX10
  • Suitable for: IHC-P, IHC-FoFr
  • Reacts with: Mouse, Rat, Human

You may also be interested in

Protein
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Recombinant Human SOX10 protein (ab114238)
Secondary
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Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)

View more associated products

Overview

  • Product name

    Anti-SOX10 antibody [EPR4007-104]
    See all SOX10 primary antibodies
  • Description

    Rabbit monoclonal [EPR4007-104] to SOX10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-P, IHC-FoFrmore details
    Unsuitable for: ICC,IP or WB
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SOX10 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P56693

  • Positive control

    • IHC-P: Human fetal brain tissue, mouse breast and cerebellum tissue IHC-Fr: Mouse and rat cerebellum
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.01% Sodium azide
    Constituents: 9% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA, 50% Tissue culture supernatant
  • Concentration information loading...
  • Purity

    Tissue culture supernatant
  • Clonality

    Monoclonal
  • Clone number

    EPR4007-104
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm

Associated products

  • Alternative Versions

    • Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free (ab220078)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SOX10 protein (ab114238)
  • Related Products

    • Recombinant Human SOX10 protein (ab114238)
    • Anti-PRAME antibody [EPR20330] (ab219650)
    • Anti-PRAME antibody [EPR20330] - BSA and Azide free (ab232571)

Applications

Our Abpromise guarantee covers the use of ab180862 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/100 - 1/500. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-FoFr 1/50.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

  • Application notes
    Is unsuitable for ICC,IP or WB.
  • Target

    • Function

      Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
    • Tissue specificity

      Expressed in fetal brain and in adult brain, heart, small intestine and colon.
    • Involvement in disease

      Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
      Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
      Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
      Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
    • Sequence similarities

      Contains 1 HMG box DNA-binding domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Target information above from: UniProt accession P56693 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 6663 Human
      • Entrez Gene: 20665 Mouse
      • Entrez Gene: 29361 Rat
      • Omim: 602229 Human
      • SwissProt: P56693 Human
      • SwissProt: Q04888 Mouse
      • SwissProt: O55170 Rat
      • Unigene: 376984 Human
      • Unigene: 276739 Mouse
      • Unigene: 10883 Rat
      see all
    • Alternative names

      • DOM antibody
      • DOM antibody
      • Dominant megacolon mouse human homolog of antibody
      • MGC15649 antibody
      • PCWH antibody
      • SOX 10 antibody
      • SOX10 antibody
      • SOX10_HUMAN antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY box 10 antibody
      • SRY box containing gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • Transcription factor SOX 10 antibody
      • Transcription factor SOX-10 antibody
      • WS2E antibody
      • WS4 antibody
      • WS4C antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

       Immunohistochemical analysis of paraffin-embedded Mouse breast tissue labeling SOX10 with ab180862 at 1/500 dilution (0.22 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse breast. The section was incubated with ab180862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

      Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

    • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

      Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

      Immunohistochemical analysis of paraffin-embedded Mouse cerebellum  tissue labeling SOX10 with ab180862 at 1/500 dilution (0.22 μg/ml) followed by a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101). Nuclear staining on mouse cerebellum.The section was incubated with ab180862 for 30 mins at room temperature. The immunostaining was performed on a Leica Biosystems BOND® RX instrument. Counterstained with Hematoxylin. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20mins.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is a ready to use Rabbit specific IHC polymer detection kit HRP/DAB (ab209101).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

      Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue labeling SOX10 with ab180862 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

      Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [EPR4007-104] (ab180862)

      Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • Anti-SOX10 antibody [EPR4007-104] (ab180862)
      Anti-SOX10 antibody [EPR4007-104] (ab180862)

    Protocols

    • Immunohistochemistry protocols

    Click here to view the general protocols

    Datasheets and documents

    • Datasheet
  • References (4)

    Publishing research using ab180862? Please let us know so that we can cite the reference in this datasheet.

    ab180862 has been referenced in 4 publications.

    • Hermanto Y  et al. Xeno-free culture for generation of forebrain oligodendrocyte precursor cells from human pluripotent stem cells. J Neurosci Res 97:828-845 (2019). PubMed: 30891830
    • Rice FL  et al. The evolution and multi-molecular properties of NF1 cutaneous neurofibromas originating from C-fiber sensory endings and terminal Schwann cells at normal sites of sensory terminations in the skin. PLoS One 14:e0216527 (2019). PubMed: 31107888
    • Zhao C  et al. Dual Requirement of CHD8 for Chromatin Landscape Establishment and Histone Methyltransferase Recruitment to Promote CNS Myelination and Repair. Dev Cell 45:753-768.e8 (2018). PubMed: 29920279
    • Wu LMN  et al. Programming of Schwann Cells by Lats1/2-TAZ/YAP Signaling Drives Malignant Peripheral Nerve Sheath Tumorigenesis. Cancer Cell 33:292-308.e7 (2018). PubMed: 29438698

    Customer reviews and Q&As

    Show All Reviews Q&A
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    1-6 of 6 Abreviews or Q&A

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX10 antibody [EPR4007-104]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Mouse Tissue sections (Optic nerve)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% TritonX-100 + 0.05% Tween20
    Specification
    Optic nerve
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 15 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX10 antibody [EPR4007-104]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Dog Tissue sections (Optic nerve)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.2% TritonX-100 + 0.05% Tween20
    Specification
    Optic nerve
    Blocking step
    (agent) for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunohistochemistry (PFA perfusion fixed frozen sections) abreview for Anti-SOX10 antibody [EPR4007-104]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (PFA perfusion fixed frozen sections)
    Sample
    Cat Tissue sections (Optic nerve)
    Antigen retrieval step
    None
    Permeabilization
    Yes - 0.05% Tween 20 + 0.2% TritonX 100
    Specification
    Optic nerve
    Blocking step
    Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 23°C
    Fixative
    Paraformaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Mar 11 2020

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX10 antibody [EPR4007-104]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Dog Tissue sections (Malignant melanoma)
    Antigen retrieval step
    Enzymatic - Buffer/Enzyme Used: EDTA
    Permeabilization
    No
    Specification
    Malignant melanoma
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted Sep 30 2019

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX10 antibody [EPR4007-104]

    Excellent
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Pig Tissue sections (Melanoma lymph node)
    Antigen retrieval step
    Heat mediated
    Permeabilization
    No
    Specification
    Melanoma lymph node
    Blocking step
    Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C
    Fixative
    Formaldehyde
    Read More

    Kibo Nam

    Verified customer

    Submitted Jul 27 2017

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX10 antibody [EPR4007-104]

    Good
    Abreviews
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Tasmanian devil Tissue sections (spinal column)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: commercial target retrieval solution
    Permeabilization
    No
    Specification
    spinal column
    Blocking step
    commercial protein block serum free as blocking agent for 30 minute(s) · Concentration: 100%
    Fixative
    Formaldehyde
    Read More

    Abcam user community

    Verified customer

    Submitted May 24 2017

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