Recombinant Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free (ab220078)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4007-104] to SOX10 - BSA and Azide free
- Suitable for: IHC-P, IHC-Fr
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free
See all SOX10 primary antibodies -
Description
Rabbit monoclonal [EPR4007-104] to SOX10 - BSA and Azide free -
Host species
Rabbit -
Tested applications
Suitable for: IHC-P, IHC-Frmore details
Unsuitable for: ICC/IF,IP or WB -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain tissue, mouse breast and cerebellum tissue IHC-Fr: Mouse and rat cerebellum tissue
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General notes
ab220078 is the carrier-free version of ab180862.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Mouse: We have preliminary internal testing data to indicate this antibody may not react with this species. Please contact us for more information.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.20
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4007-104 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-SOX10 antibody [EPR4007-104] (ab180862)
- Alexa Fluor® 488 Anti-SOX10 antibody [EPR4007-104] (ab311012)
- Alexa Fluor® 647 Anti-SOX10 antibody [EPR4007-104] (ab311140)
- Alexa Fluor® 594 Anti-SOX10 antibody [EPR4007-104] (ab311777)
- Alexa Fluor® 568 Anti-SOX10 antibody [EPR4007-104] (ab313058)
- Alexa Fluor® 555 Anti-SOX10 antibody [EPR4007-104] (ab313258)
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab220078 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
IHC-P | (2) |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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IHC-Fr | (1) |
Use at an assay dependent concentration.
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Notes |
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IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
IHC-Fr
Use at an assay dependent concentration. |
Target
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Function
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. -
Tissue specificity
Expressed in fetal brain and in adult brain, heart, small intestine and colon. -
Involvement in disease
Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6663 Human
- Entrez Gene: 20665 Mouse
- Entrez Gene: 29361 Rat
- Omim: 602229 Human
- SwissProt: P56693 Human
- SwissProt: Q04888 Mouse
- SwissProt: O55170 Rat
- Unigene: 376984 Human
see all -
Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling SOX10 with purified ab180862 at 1/500 (0.22 µg/ml). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862). -
Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab180862). -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/500 (0.22 µg/ml). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862). -
Immunohistochemistry (Frozen) analysis of rat cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide ab180862). -
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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This IHC data was generated using the same anti-SOX10 antibody clone, EPR4007-104, in a different buffer formulation (cat# ab180862).
Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue labeling SOX10 with ab180862 at 1/100 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
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This IHC data was generated using the same anti-SOX10 antibody clone, EPR4007-104, in a different buffer formulation (cat# ab180862).
Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.
Heat mediated antigen retrieval was performed before commencing with IHC staining protocol.
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab220078 has not yet been referenced specifically in any publications.