Recombinant
RabMAb

Recombinant Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free (ab220078)

Overview

  • Product name

    Anti-SOX10 antibody [EPR4007-104] - BSA and Azide free
    See all SOX10 primary antibodies
  • Description

    Rabbit monoclonal [EPR4007-104] to SOX10 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: IHC-Pmore details
    Unsuitable for: ICC,IP or WB
  • Species reactivity

    Reacts with: Mouse, Human
  • Immunogen

    Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human SOX10 aa 400 to the C-terminus (Cysteine residue). The exact sequence is proprietary.
    Database link: P56693

  • Positive control

    • Human fetal brain tissue, mouse breast and cerebellum tissue
  • General notes

    ab220078 is the carrier-free version of ab180862 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab220078 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Mouse: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab220078 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
  • Application notes
    Is unsuitable for ICC,IP or WB.
  • Target

    • Function

      Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
    • Tissue specificity

      Expressed in fetal brain and in adult brain, heart, small intestine and colon.
    • Involvement in disease

      Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
      Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
      Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
      Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
    • Sequence similarities

      Contains 1 HMG box DNA-binding domain.
    • Cellular localization

      Cytoplasm. Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • DOM antibody
      • DOM antibody
      • Dominant megacolon mouse human homolog of antibody
      • MGC15649 antibody
      • PCWH antibody
      • SOX 10 antibody
      • SOX10 antibody
      • SOX10_HUMAN antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY box 10 antibody
      • SRY box containing gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • Transcription factor SOX 10 antibody
      • Transcription factor SOX-10 antibody
      • WS2E antibody
      • WS4 antibody
      • WS4C antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling SOX10 with purified ab180862 at 1/500 (0.22 µg/ml). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab180862 at 1/500 (0.22 µg/ml). Antigen retrieval was heat mediated with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 20 minutes. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain.
      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).

    • Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab180862).

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • This IHC data was generated using the same anti-SOX10 antibody clone, EPR4007-104, in a different buffer formulation (cat# ab180862).

      Immunohistochemical analysis of paraffin-embedded Human fetal brain tissue labeling SOX10 with ab180862 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    • This IHC data was generated using the same anti-SOX10 antibody clone, EPR4007-104, in a different buffer formulation (cat# ab180862).

      Immunohistochemical analysis of paraffin-embedded Human melanoma tissue labeling SOX10 with ab180862 at 1/100 dilution.

      Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

    References

    ab220078 has not yet been referenced specifically in any publications.

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