Recombinant
RabMAb

Recombinant Anti-SOX10 antibody [EPR4007] (ab155279)

Rabbit recombinant monoclonal SOX10 antibody [EPR4007]. Validated in WB, Flow Cyt, ICC/IF and tested in Mouse, Rat, Human. Cited in 20 publication(s). Independently reviewed in 4 review(s).

Overview

  • Product name

    Anti-SOX10 antibody [EPR4007]
    See all SOX10 primary antibodies
  • Description

    Rabbit monoclonal [EPR4007] to SOX10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ICC/IF, WB, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
  • Immunogen

    corresponding to Human SOX10 aa 400 to the C-terminus.
    Database link: P56693

  • Positive control

    • WB: Human brain, SH-SY5Y, A-375, C6, Mouse brain, Neuro-2a, and Rat brain lysates; ICC/IF: A-375 cells; Flow Cyt: A-375 cells.
  • General notes

     

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab155279 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50.

For unpurified use at 1/250 - 1/500.

WB 1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
Flow Cyt 1/200.

Target

  • Function

    Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
  • Tissue specificity

    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • Involvement in disease

    Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
    Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
    Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • DOM antibody
    • DOM antibody
    • Dominant megacolon mouse human homolog of antibody
    • MGC15649 antibody
    • PCWH antibody
    • SOX 10 antibody
    • SOX10 antibody
    • SOX10_HUMAN antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY box 10 antibody
    • SRY box containing gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • Transcription factor SOX 10 antibody
    • Transcription factor SOX-10 antibody
    • WS2E antibody
    • WS4 antibody
    • WS4C antibody
    see all

Images

  • All lanes : Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution (Purified)

    Lane 1 : Human brain lysates
    Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
    Lane 3 : Mouse brain lysates
    Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
    Lane 5 : Rat brain lysates

    Lysates/proteins at 20 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution

    Predicted band size: 49 kDa
    Observed band size: 56-75 kDa
    why is the actual band size different from the predicted?



    The bands observed are consistent with what have been described in PMID: 21423190

  • Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1:50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Flow cytometry analysis of A-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution ( 1ug/ml) (Red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488)(ab150077) )(1/2000 dilution) was used as the secondary antibody. Rabbit monoclonal IgG (Black)(ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.

  • All lanes : Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution (unpurified)

    Lane 1 : A-375 (human malignant melanoma cell line) lysate
    Lane 2 : C6 (rat glial tumor cell line) lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 49 kDa

  • Immunofluorescent staining of A-375 (human malignant melanoma cell line) cells labeling SOX10 with ab155279 (unpurified) at a 1/250 dilution.

References

This product has been referenced in:

  • Pitarokoili K  et al. Intrathecal triamcinolone acetonide exerts anti-inflammatory effects on Lewis rat experimental autoimmune neuritis and direct anti-oxidative effects on Schwann cells. J Neuroinflammation 16:58 (2019). Read more (PubMed: 30851725) »
  • Pandamooz S  et al. Enhancing the expression of neurotrophic factors in epidermal neural crest stem cells by valproic acid: A potential candidate for combinatorial treatment. Neurosci Lett 704:8-14 (2019). Read more (PubMed: 30904572) »
See all 26 Publications for this product

Customer reviews and Q&As

1-5 of 5 Abreviews or Q&A

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (Skin, melanocytes)
Permeabilization
No
Specification
Skin, melanocytes
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Mar 20 2019

Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Oligodendrocytes Precursors Cells primary mouse ce)
Specification
Oligodendrocytes Precursors Cells primary mouse ce
Blocking step
BSA as blocking agent for 20 minute(s) · Concentration: 2% · Temperature: 24°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Dec 11 2017

Application
Other
Sample
Mouse Cell (Mouse brain)

Abcam user community

Verified customer

Submitted Nov 21 2017

Application
Immunohistochemistry (PFA perfusion fixed frozen sections)
Sample
Mouse Tissue sections (cerebral cortex)
Antigen retrieval step
None
Permeabilization
Yes - 0.5% Triton X-100
Specification
cerebral cortex
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 25°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Jul 25 2017

Application
Flow Cytometry
Fixation
Frozen
Permeabilization
Yes - 0.1% Triton-X
Sample
Human Cell (Brain)
Specification
Brain
Gating Strategy
forward scatter/side scatter (all cells)
Preparation
Cell harvesting/tissue preparation method: Homogenized frozen tissue
Sample buffer: Tris/EDTA/sucrose/Triton

Abcam user community

Verified customer

Submitted Sep 03 2013

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