Recombinant Anti-SOX10 antibody [EPR4007] (ab155279)
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279)](https://www.abcam.com/ps/products/155/ab155279/Images/ab155279-6-anti-sox10-antibody-epr4007-104-immunohistochemistry-frozen-cerebellum-mouse.jpg "Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279)")
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR4007] to SOX10
- Suitable for: ICC/IF, IHC-FoFr, Flow Cyt (Intra), WB
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX10 antibody [EPR4007]
See all SOX10 primary antibodies -
Description
Rabbit monoclonal [EPR4007] to SOX10 -
Host species
Rabbit -
Tested applications
Suitable for: ICC/IF, IHC-FoFr, Flow Cyt (Intra), WBmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: Human brain, SH-SY5Y, A375, Mouse brain, Neuro-2a, and Rat brain lysates; ICC/IF: C6 cells. Flow Cyt (intra): A-375 cells. IHC-Fr: Mouse cerebellum
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR4007 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab155279 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| ICC/IF | (3) |
1/500.
For unpurified use at 1/250 - 1/500. |
| IHC-FoFr | (1) |
1/50.
Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
| Flow Cyt (Intra) |
1/200.
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| WB | (1) |
1/1000 - 1/10000. Predicted molecular weight: 49 kDa.
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| Notes |
|---|
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ICC/IF
1/500. For unpurified use at 1/250 - 1/500. |
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IHC-FoFr
1/50. Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20). |
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Flow Cyt (Intra)
1/200. |
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WB
1/1000 - 1/10000. Predicted molecular weight: 49 kDa. |
Target
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Function
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. -
Tissue specificity
Expressed in fetal brain and in adult brain, heart, small intestine and colon. -
Involvement in disease
Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6663 Human
- Entrez Gene: 20665 Mouse
- Entrez Gene: 29361 Rat
- Omim: 602229 Human
- SwissProt: P56693 Human
- SwissProt: Q04888 Mouse
- SwissProt: O55170 Rat
- Unigene: 376984 Human
see all -
Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
see all
Images
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Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [EPR4007] (ab155279)
Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab155279 at 1/50 (2.2 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.
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![Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)](https://www.abcam.com/ps/products/155/ab155279/Images/ab155279-469391-antisox10-antibody-epr4007-western-blot-human.jpg)
Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)Anti-SOX10 antibody [EPR4007] (ab155279) at 1/2000 dilution + A375 (Human malignant melanoma epithelial cell) whole cell lysate at 15 µg
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/20000 dilution
Predicted band size: 49 kDa
Observed band size: 56-75 kDa why is the actual band size different from the predicted?
Exposure time: 3 secondsBlocking and diluting buffer: 5% NFDM/TBST
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![Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)](https://www.abcam.com/ps/products/155/ab155279/Images/ab155279-351369-anti-sox10-antibody-epr4007-westernblot-human-mouse-rat.jpg)
Western blot - Anti-SOX10 antibody [EPR4007] (ab155279)All lanes : Anti-SOX10 antibody [EPR4007] (ab155279) at 1/1000 dilution (Purified)
Lane 1 : Human brain lysates
Lane 2 : SH-SY5Y (Human neuroblastoma epithelial cell) whole cell lysates
Lane 3 : Mouse brain lysates
Lane 4 : Neuro-2a (Mouse neuroblastoma neuroblast) whole cell lysates
Lane 5 : Rat brain lysates
Lysates/proteins at 20 µg per lane.
Secondary
All lanes : Goat Anti-Rabbit IgG (HRP) with minimal cross-reactivity with human IgG at 1/2000 dilution
Predicted band size: 49 kDa
Observed band size: 56-75 kDa why is the actual band size different from the predicted?The bands observed are consistent with what have been described in PMID: 21423190
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![Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (ab155279)](https://www.abcam.com/ps/products/155/ab155279/Images/ab155279-4-anti-sox10-antibody-epr4007-immunocytochemistry-c6-rat.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [EPR4007] (ab155279)Immunocytochemistry/ Immunofluorescence analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab155279 at 1:50 dilution (3.6 µg/ml). Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control. -
![Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (ab155279)](https://www.abcam.com/ps/products/155/ab155279/Images/ab155279-276939-anti-sox10-antibody-epr4007-flow-cytometry.jpg)
Flow Cytometry (Intracellular) - Anti-SOX10 antibody [EPR4007] (ab155279)Intracellular Flow Cytometry analysis ofA-375 (human malignant melanoma cell line) cells labeling with purified ab155279 at 1/200 dilution (1ug/ml) (Red). Cells were fixed with4% paraformaldehydeand permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488) (ab150077) (1/2000 dilution) was used as the secondary antibody.Rabbit monoclonal IgG (Black) (ab172730) was used as a isotype control.Cell without incubation with primary antibody and secondary antibody (Blue) were used as unlabeled control.
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![Anti-SOX10 antibody [EPR4007] (ab155279)](https://www.abcam.com/ps/products/155/ab155279/Images/ab155279-7-benefits-of-recombinant-antibodies.png)
Anti-SOX10 antibody [EPR4007] (ab155279)
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (63)
ab155279 has been referenced in 63 publications.
- Tan M et al. Maternal vitamin A deficiency impairs cholinergic and nitrergic neurons, leading to gastrointestinal dysfunction in rat offspring via RARß. Life Sci 264:118688 (2021). PubMed: 33130074
- Bargagna-Mohan P et al. Corneal nonmyelinating Schwann cells illuminated by single-cell transcriptomics and visualized by protein biomarkers. J Neurosci Res 99:731-749 (2021). PubMed: 33197966
- Coletto E et al. Dysregulation of epithelial ion transport and neurochemical changes in the colon of a parkinsonian primate. NPJ Parkinsons Dis 7:9 (2021). PubMed: 33479243
- Yuan H et al. Premigratory neural crest stem cells generate enteric neurons populating the mouse colon and regulating peristalsis in tissue-engineered intestine. Stem Cells Transl Med 10:922-938 (2021). PubMed: 33481357
- Coassolo S et al. Citrullination of pyruvate kinase M2 by PADI1 and PADI3 regulates glycolysis and cancer cell proliferation. Nat Commun 12:1718 (2021). PubMed: 33741961