Application
Flow Cytometry
Fixation
Frozen
Permeabilization
Yes - 0.1% Triton-X
Sample
Human Cell (Brain)
Specification
Brain
Gating Strategy
forward scatter/side scatter (all cells)
Preparation
Cell harvesting/tissue preparation method: Homogenized frozen tissue
Sample buffer: Tris/EDTA/sucrose/Triton
Sample buffer: Tris/EDTA/sucrose/Triton
Other product details
Incubation time
30 minute(s) · Temperature: 4°C · Diluent: PBS with BSA
Dilution
1/500
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen goat anti-rabbit 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor 647
Dilution
1/500
Additional data
Abcam response
Thank you so much for testing this antibody in flow cytometry.
We appreciate very much your efforts, but are sorry to hear that the antibody did not work well in this application. The customer received a discount on a future antibody purchase for testing this product in flow cytometry. We would welcome additional feedback from other customers as well, but we would also suggest including a reference sample, such as an isotype control.
Abcam user community
Verified customer
Submitted Sep 03 2013