Anti-SOX10 antibody [SOX10/1074] (ab216020)
Key features and details
- Mouse monoclonal [SOX10/1074] to SOX10
- Suitable for: Protein Array, IHC-P, WB
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Get better batch-to-batch reproducibility with a recombinant antibody
- Research with confidence – consistent and reproducible results with every batch
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- Success from the first experiment – confirmed specificity through extensive validation
- Ethical standards compliant – production is animal-free
Overview
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Product name
Anti-SOX10 antibody [SOX10/1074]
See all SOX10 primary antibodies -
Description
Mouse monoclonal [SOX10/1074] to SOX10 -
Host species
Mouse -
Tested applications
Suitable for: Protein Array, IHC-P, WBmore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment -
Immunogen
Recombinant fragment within Human SOX10 aa 100-300. The exact immunogen sequence used to generate this antibody is proprietary information. If additional detail on the immunogen is needed to determine the suitability of the antibody for your needs, please contact our Scientific Support team to discuss your requirements.
Database link: P56693 -
Positive control
- Human melanoma tissue; Recombinant SOX10 protein; A375 cell lysate; Mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.05% Sodium azide
Constituents: 99% PBS, 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
ab216020 was purified from Bioreactor Concentrate by Protein A/G. -
Clonality
Monoclonal -
Clone number
SOX10/1074 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab216020 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Protein Array |
Use at an assay dependent concentration.
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IHC-P |
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
(Primary incubation for 30 minutes at RT). |
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WB |
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa.
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Notes |
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Protein Array
Use at an assay dependent concentration. |
IHC-P
Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. (Primary incubation for 30 minutes at RT). |
WB
Use a concentration of 1 - 2 µg/ml. Predicted molecular weight: 49 kDa. |
Target
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Function
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. -
Tissue specificity
Expressed in fetal brain and in adult brain, heart, small intestine and colon. -
Involvement in disease
Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6663 Human
- Entrez Gene: 20665 Mouse
- Omim: 602229 Human
- SwissProt: P56693 Human
- SwissProt: Q04888 Mouse
- Unigene: 376984 Human
- Unigene: 276739 Mouse
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Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
see all
Images
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Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma tissue labeling SOX10 with ab216020 at 1 µg/ml.
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All lanes : Anti-SOX10 antibody [SOX10/1074] (ab216020) at 1 µg/ml
Lane 1 : Recombinant fragment Human SOX10 aa 115-269
Lane 2 : A375 cell lysate
Predicted band size: 49 kDa -
ab216020 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
Immunohistochemical analysis of formalin-fixed, paraffin-embedded mouse brain tissue labeling SOX10 with ab216020 at 1 µg/ml.
Protocols
Datasheets and documents
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Datasheet download
References (8)
ab216020 has been referenced in 8 publications.
- Vittoria MA et al. Inactivation of the Hippo tumor suppressor pathway promotes melanoma. Nat Commun 13:3732 (2022). PubMed: 35768444
- Prabhakar S et al. Gene replacement therapy in a schwannoma mouse model of neurofibromatosis type 2. Mol Ther Methods Clin Dev 26:169-180 (2022). PubMed: 35846573
- Ou MY et al. Dedifferentiated Schwann cell-derived TGF-β3 is essential for the neural system to promote wound healing. Theranostics 12:5470-5487 (2022). PubMed: 35910794
- Sun Y et al. Ninj2 regulates Schwann cells development by interfering laminin-integrin signaling. Theranostics 12:7307-7318 (2022). PubMed: 36438492
- Grigore F et al. BRAF inhibition in melanoma is associated with the dysregulation of histone methylation and histone methyltransferases. Neoplasia 22:376-389 (2020). PubMed: 32629178
- Duman M et al. EEF1A1 deacetylation enables transcriptional activation of remyelination. Nat Commun 11:3420 (2020). PubMed: 32647127
- De Logu F et al. Macrophages and Schwann cell TRPA1 mediate chronic allodynia in a mouse model of complex regional pain syndrome type I. Brain Behav Immun 88:535-546 (2020). PubMed: 32315759
- De Logu F et al. Schwann cell TRPA1 mediates neuroinflammation that sustains macrophage-dependent neuropathic pain in mice. Nat Commun 8:1887 (2017). PubMed: 29192190