Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)
![Protein Array - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)](https://www.abcam.com/ps/products/212/ab212843/Images/ab212843-336707-anti-sox10-antibody-sox10-991-protein-array.jpg "Protein Array - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)")
Key features and details
- Mouse monoclonal [SOX10/991] to SOX10 - BSA and Azide free
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Mouse, Human, Recombinant fragment
- Isotype: IgG2b
Overview
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Product name
Anti-SOX10 antibody [SOX10/991] - BSA and Azide free
See all SOX10 primary antibodies -
Description
Mouse monoclonal [SOX10/991] to SOX10 - BSA and Azide free -
Host species
Mouse -
Tested applications
Suitable for: WB, IHC-P, Protein Arraymore details -
Species reactivity
Reacts with: Mouse, Human, Recombinant fragment
Predicted to work with: Rat, Chicken, Pig
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Immunogen
Recombinant fragment (His-tag) corresponding to Human SOX10 aa 100-300.
Database link: P56693 -
Positive control
- WB: A375 cell lysate; Recombinant human SOX10 protein. IHC-P: Human melanoma and mouse brain tissue.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Constituent: 100% PBS -
Carrier free
Yes -
Concentration information loading... -
Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
SOX10/991 -
Isotype
IgG2b -
Light chain type
kappa -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Conjugation kits
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Isotype control
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Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab212843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa.
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| IHC-P | (2) |
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20-30 minutes. |
| Protein Array |
Use at an assay dependent concentration.
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| Notes |
|---|
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WB
Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa. |
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IHC-P
Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20-30 minutes. |
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Protein Array
Use at an assay dependent concentration. |
Target
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Function
Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia. -
Tissue specificity
Expressed in fetal brain and in adult brain, heart, small intestine and colon. -
Involvement in disease
Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Cytoplasm. Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 395573 Chicken
- Entrez Gene: 6663 Human
- Entrez Gene: 20665 Mouse
- Entrez Gene: 414903 Pig
- Entrez Gene: 29361 Rat
- Omim: 602229 Human
- SwissProt: Q9W757 Chicken
- SwissProt: P56693 Human
see all -
Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
see all
Images
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Protein Array - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)This data was produced with ab218522, the same antibody in a different formulation with BSA and Azide.
ab218522 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29. -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)](https://www.abcam.com/ps/products/212/ab212843/Images/ab212843-264420-anti-sox10-antibody-sox10-991-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma tissue labeling SOX10 with ab212843 at 1 µg/ml.
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![Western blot - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)](https://www.abcam.com/ps/products/212/ab212843/Images/ab212843-264419-anti-sox10-antibody-sox10-991-western-blot.jpg)
Western blot - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)All lanes : Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843) at 1 µg/ml
Lane 1 : Recombinant fragment of Human SOX10
Lane 2 : A375 cell lysate
Predicted band size: 50 kDa -
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)](https://www.abcam.com/ps/products/212/ab212843/Images/ab212843-304253-anti-sox10-antibody-sox10-991-immunohistochemistry.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843)Formalin-fixed, paraffin-embedded mouse brain tissue stained for SOX10 using ab212843 at 1 ug/ml in immunohistochemical analysis.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (8)
ab212843 has been referenced in 8 publications.
- Khaliq M et al. Epigenetic modulation reveals differentiation state specificity of oncogene addiction. Nat Commun 12:1536 (2021). PubMed: 33750776
- Shao Y et al. Effect of Sox10 on remyelination of the hippocampus in cuprizone-induced demyelinated mice. Brain Behav 10:e01623 (2020). PubMed: 32363773
- Wenzina J et al. Inhibition of p38/MK2 Signaling Prevents Vascular Invasion of Melanoma. J Invest Dermatol 140:878-890.e5 (2020). PubMed: 31622599
- Liu LP et al. Generation of Human iMelanocytes from Induced Pluripotent Stem Cells through a Suspension Culture System. STAR Protoc 1:100004 (2020). PubMed: 33111066
- Ferdoushi A et al. Schwann Cell Stimulation of Pancreatic Cancer Cells: A Proteomic Analysis. Front Oncol 10:1601 (2020). PubMed: 32984024
- Liu LP et al. Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation. Cell Rep 27:455-466.e5 (2019). PubMed: 30970249
- Huang GX et al. Primary malignant gastrointestinal neuroectodermal tumor occurring in the ileum with intra-abdominal granulomatous nodules: A case report and review of the literature. Oncol Lett 17:3899-3909 (2019). PubMed: 30930990
- Corces MR et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14:959-962 (2017). PubMed: 28846090