Key features and details
- Mouse monoclonal [SOX10/991] to SOX10 - BSA and Azide free
- Suitable for: WB, IHC-P, Protein Array
- Reacts with: Mouse, Human
- Isotype: IgG2b
Product nameAnti-SOX10 antibody [SOX10/991] - BSA and Azide free
See all SOX10 primary antibodies
DescriptionMouse monoclonal [SOX10/991] to SOX10 - BSA and Azide free
Tested applicationsSuitable for: WB, IHC-P, Protein Arraymore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Chicken, Pig
- WB: A375 cell lysate; Recombinant human SOX10 protein. IHC-P: Human melanoma and mouse brain tissue.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferConstituent: 100% PBS
Concentration information loading...
PurityProtein A purified
Light chain typekappa
Our Abpromise guarantee covers the use of ab212843 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 0.5 - 1 µg/ml. Predicted molecular weight: 50 kDa.|
|IHC-P||Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
Boil tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 min followed by cooling at RT for 20-30 minutes.
|Protein Array||Use at an assay dependent concentration.|
FunctionTranscription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
Tissue specificityExpressed in fetal brain and in adult brain, heart, small intestine and colon.
Involvement in diseaseDefects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
Cellular localizationCytoplasm. Nucleus.
- Information by UniProt
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
This data was produced with ab218522, the same antibody in a different formulation with BSA and Azide.
ab218522 was tested in protein array against over 19000 different full-length human proteins.
Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target.
A MAb is specific to its intended target if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.
Immunohistochemical analysis of formalin-fixed, paraffin-embedded human melanoma tissue labeling SOX10 with ab212843 at 1 µg/ml.
All lanes : Anti-SOX10 antibody [SOX10/991] - BSA and Azide free (ab212843) at 1 µg/ml
Lane 1 : Recombinant fragment of Human SOX10
Lane 2 : A375 cell lysate
Predicted band size: 50 kDa
Formalin-fixed, paraffin-embedded mouse brain tissue stained for SOX10 using ab212843 at 1 ug/ml in immunohistochemical analysis.
ab212843 has been referenced in 3 publications.
- Liu LP et al. Therapeutic Potential of Patient iPSC-Derived iMelanocytes in Autologous Transplantation. Cell Rep 27:455-466.e5 (2019). PubMed: 30970249
- Huang GX et al. Primary malignant gastrointestinal neuroectodermal tumor occurring in the ileum with intra-abdominal granulomatous nodules: A case report and review of the literature. Oncol Lett 17:3899-3909 (2019). PubMed: 30930990
- Corces MR et al. An improved ATAC-seq protocol reduces background and enables interrogation of frozen tissues. Nat Methods 14:959-962 (2017). PubMed: 28846090