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RecombinantRabMAb

Recombinant Anti-SOX10 antibody [SP267] (ab227680)

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Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)
  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
  • Western blot - Anti-SOX10 antibody [SP267] (ab227680)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
  • Anti-SOX10 antibody [SP267] (ab227680)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP267] to SOX10
  • Suitable for: Flow Cyt (Intra), IHC-FoFr, IHC-P, WB, ICC/IF
  • Reacts with: Mouse, Rat, Human

Conjugates logo Related conjugates and formulations

Alexa Fluor® 488 Alexa Fluor® 647 Carrier Free PE

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View more associated products

Overview

  • Product name

    Anti-SOX10 antibody [SP267]
    See all SOX10 primary antibodies
  • Description

    Rabbit monoclonal [SP267] to SOX10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: Flow Cyt (Intra), IHC-FoFr, IHC-P, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Chicken, Pig
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • WB: A-375 cell lysate. IHC-P: Human melanoma tissue; mouse and rat breast tissue. Flow Cyt (intra): A-375, C6, and B16-F0 cells. ICC/IF: A-375, C6, and B16-F0 cells. IHC-Fr: Mouse cerebellum.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.20
    Preservative: 0.1% Sodium azide
    Constituents: 1% BSA, PBS
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    SP267
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-SOX10 antibody [SP267] - C-terminal, prediluted (ab228188)
    • Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
    • Alexa Fluor® 488 Anti-SOX10 antibody [SP267] (ab270150)
    • Alexa Fluor® 647 Anti-SOX10 antibody [SP267] (ab270151)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SOX10 protein (ab114238)
  • Related Products

    • Anti-PRAME antibody [EPR20330] (ab219650)
    • Anti-PRAME antibody [EPR20330] - BSA and Azide free (ab232571)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt (Intra)
1/20 - 1/200.
IHC-FoFr
1/50.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

WB (1)
1/400. Predicted molecular weight: 49 kDa.

Primary antibody incubation for 1 hour at room temperature.

ICC/IF
1/25.
Notes
Flow Cyt (Intra)
1/20 - 1/200.
IHC-FoFr
1/50.

Perform heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20).

IHC-P
1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Primary antibody incubation for 10 minutes at room temperature.

WB
1/400. Predicted molecular weight: 49 kDa.

Primary antibody incubation for 1 hour at room temperature.

ICC/IF
1/25.

Target

  • Function

    Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
  • Tissue specificity

    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • Involvement in disease

    Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
    Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
    Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P56693 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 395573 Chicken
    • Entrez Gene: 6663 Human
    • Entrez Gene: 20665 Mouse
    • Entrez Gene: 414903 Pig
    • Entrez Gene: 29361 Rat
    • Omim: 602229 Human
    • SwissProt: Q9W757 Chicken
    • SwissProt: P56693 Human
    • SwissProt: Q04888 Mouse
    • SwissProt: A5A763 Pig
    • SwissProt: O55170 Rat
    • Unigene: 376984 Human
    • Unigene: 276739 Mouse
    • Unigene: 10883 Rat
    see all
  • Alternative names

    • DOM antibody
    • DOM antibody
    • Dominant megacolon mouse human homolog of antibody
    • MGC15649 antibody
    • PCWH antibody
    • SOX 10 antibody
    • SOX10 antibody
    • SOX10_HUMAN antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY (sex determining region Y) box 10 antibody
    • SRY box 10 antibody
    • SRY box containing gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • SRY related HMG box gene 10 antibody
    • Transcription factor SOX 10 antibody
    • Transcription factor SOX-10 antibody
    • WS2E antibody
    • WS4 antibody
    • WS4C antibody
    see all

Images

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)

    ab227680 staining SOX10 in A375 cells, with negative expression in HeLa cells. The cells were fixed with 4% formaldehyde (10 min), permeabilised with 0.1% Triton x-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab227680 at 1 µg/ml and ab7291, Mouse monoclonal [DM1A] to alpha Tubulin at 0.5 µg/ml. Cells were then incubated with ab150081, Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (Alexa Fluor® 488), pre-adsorbed at 1/1000 dilution (shown in green) and ab150119, Goat polyclonal Secondary Antibody to Mouse IgG - H&L (Alexa Fluor® 647), pre-adsorbed at 1/1000 dilution (shown in red). Nuclear DNA was labelled with DAPI (shown in blue).

    Image was acquired with a high-content analyser (Operetta CLS, Perkin Elmer) and a maximum intensity projection of confocal sections is shown.

    This product also work with 100% methanol (5 min) fixation under the same testing conditions.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human melanoma tissue sections labeling SOX10 with ab227680 at 1/100 dilution (0.97 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human melanoma, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227680 for 10 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
    Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
  • Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [SP267] (ab227680)
    Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-SOX10 antibody [SP267] (ab227680)

    Immunohistochemistry (Frozen) analysis of mouse cerebellum tissue sections labeling SOX10 with purified ab227680 at 1/50 (1.4 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) at 1/1000 (2 µg/ml) was used as the secondary antibody. Sections were fixed with 4% paraformaldehyde and permeabilised with 0.2% Triton X-100. Negative control: PBS instead of the primary antibody. DAPI (blue) was used as nuclear counterstain. Heat mediated antigen retrieval using sodium citrate buffer (10mM citrate pH 6.0 + 0.05% Tween-20) was performed.

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat breast tissue sections labeling SOX10 with ab227680 at 1/100 dilution (0.97 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat breast, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227680 for 10 mins at room temperature.

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)
    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)

    Intracellular Flow Cytometry analysis of C6 (Rat glial tumor glial cell) cells labeling SOX10 with purified ab227680 at 1/20 dilution (3.75µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. 

     

     

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse breast tissue sections labeling SOX10 with ab227680 at 1/100 dilution (0.97 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
    The section was incubated with ab227680 for 10 mins at room temperature.

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)

    Immunocytochemistry/ Immunofluorescence analysis of B16-F0 (mouse melanoma epithelial cell-like) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)
    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)

    Intracellular Flow Cytometry analysis of B16-F0 (Mouse melanoma epithelial cell-like) cells labeling SOX10 with purified ab227680 at 1/200 dilution (0.375µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. 

     

     

  • Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)
    Flow Cytometry (Intracellular) - Anti-SOX10 antibody [SP267] (ab227680)

    Intracellular Flow Cytometry analysis of A-375 (Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227680 at 1/200 dilution (0.375µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue. 

     

     

  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)
    Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)

    Immunocytochemistry/ Immunofluorescence analysis of A-375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

  • Western blot - Anti-SOX10 antibody [SP267] (ab227680)
    Western blot - Anti-SOX10 antibody [SP267] (ab227680)
    Anti-SOX10 antibody [SP267] (ab227680) at 1/400 dilution + A-375 (human malignant melanoma cell line) cell lysate

    Predicted band size: 49 kDa

  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

    Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227680 at 1/100 dilution in immunohistochemical analysis.

  • Anti-SOX10 antibody [SP267] (ab227680)
    Anti-SOX10 antibody [SP267] (ab227680)

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (2)

Publishing research using ab227680? Please let us know so that we can cite the reference in this datasheet.

ab227680 has been referenced in 2 publications.

  • Liu JL  et al. Increased nuclear translation of YAP might act as a potential therapeutic target for NF1-related plexiform neurofibroma. Int J Med Sci 18:2008-2016 (2021). PubMed: 33850471
  • Chen W  et al. The Neurogenic Compound P7C3 Regulates the Aerobic Glycolysis by Targeting Phosphoglycerate Kinase 1 in Glioma. Front Oncol 11:644492 (2021). PubMed: 34221965

Customer reviews and Q&As

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1-4 of 4 Abreviews or Q&A

Western blot abreview for Anti-SOX10 antibody [SP267]

Excellent
Abreviews
Abreviews
abreview image
Application
Western blot
Sample
Mouse Tissue lysate - whole (Brain)
Gel Running Conditions
Non-reduced Denaturing (4-20%)
Loading amount
15 µg
Specification
Brain
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Read More

Abcam user community

Verified customer

Submitted Jan 11 2022

Flow Cytometry abreview for Anti-SOX10 antibody [SP267]

Excellent
Abreviews
Abreviews
abreview image
Application
Flow Cytometry
Sample
Human Cell (Glioma)
Permeabilization
Yes - 30min 0.5% Triton-x PBS
Specification
Glioma
Preparation
Cell harvesting/tissue preparation method: In vitro patient-derrived cells
Sample buffer: PBS
Fixation
Paraformaldehyde
Read More

Lucy Brooks

Verified customer

Submitted Oct 29 2020

Immunohistochemistry free floating abreview for Anti-SOX10 antibody [SP267]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Mouse brain)
Specification
Mouse brain
Read More

Lucy Brooks

Verified customer

Submitted Oct 29 2020

Immunohistochemistry free floating abreview for Anti-SOX10 antibody [SP267]

Excellent
Abreviews
Abreviews
abreview image
Application
Immunohistochemistry free floating
Sample
Mouse Tissue sections (Brain cortex)
Specification
Brain cortex
Read More

Abcam user community

Verified customer

Submitted Sep 10 2020

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