Name: Anti-SOX10 antibody [SP267]
Brand: RabMAb
SKU: ab227680

Product name

Anti-SOX10 antibody [SP267]
See all SOX10 primary antibodies
Description

Rabbit monoclonal [SP267] to SOX10
Host species

Rabbit
Tested applications

Suitable for: IHC-P, WB, Flow Cyt, ICC/IFmore details
Species reactivity

Reacts with: Human
Predicted to work with: Mouse, Rat, Chicken, Pig
Immunogen

Synthetic peptide within Human SOX10 aa 350-450. The exact sequence is proprietary.
Database link: P56693
Positive control
- WB: A-375 cell lysate. IHC-P: Human melanoma tissue, melanoma tissue, mouse and rat breast tissue. FC: A375, C6, and B16-F0 cells. ICC/IF: A375, C6, and B16-F0 cells.
General notes

Our RabMAb^® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab^® patents.
This product is a recombinant rabbit monoclonal antibody.

Form

Liquid
Storage instructions

Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage buffer

pH: 7.2
Preservative: 0.1% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
Purity

Protein A purified
Clonality

Monoclonal
Clone number

SP267
Isotype

IgG
Research areas

Alternative Versions
- Anti-SOX10 antibody [SP267] - C-terminal, prediluted (ab228188)
- Anti-SOX10 antibody [SP267] - BSA and Azide free (ab245760)
Compatible Secondaries
- Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
- Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
Isotype control
- Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
Recombinant Protein
- Recombinant Human SOX10 protein (ab114238)
Related Products
- Anti-PRAME antibody [EPR20330] (ab219650)
- Anti-PRAME antibody [EPR20330] - BSA and Azide free (ab232571)

Our Abpromise guarantee covers the use of ab227680 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application	Abreviews	Notes
IHC-P		1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Primary antibody incubation for 10 minutes at room temperature.
WB		1/400. Predicted molecular weight: 49 kDa. Primary antibody incubation for 1 hour at room temperature.
Flow Cyt		1/20 - 1/200.
ICC/IF		1/25.

Function

Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
Tissue specificity

Expressed in fetal brain and in adult brain, heart, small intestine and colon.
Involvement in disease

Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
Sequence similarities

Contains 1 HMG box DNA-binding domain.
Cellular localization

Cytoplasm. Nucleus.
Target information above from: UniProt accession P56693 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010) .

Information by UniProt
Database links
- Entrez Gene: 395573 Chicken
- Entrez Gene: 6663 Human
- Entrez Gene: 20665 Mouse
- Entrez Gene: 414903 Pig
- Entrez Gene: 29361 Rat
- Omim: 602229 Human
- SwissProt: Q9W757 Chicken
- SwissProt: P56693 Human
- SwissProt: Q04888 Mouse
- SwissProt: A5A763 Pig
- SwissProt: O55170 Rat
- Unigene: 376984 Human
- Unigene: 276739 Mouse
- Unigene: 10883 Rat
see all
Alternative names
- DOM antibody
- DOM antibody
- Dominant megacolon mouse human homolog of antibody
- MGC15649 antibody
- PCWH antibody
- SOX 10 antibody
- SOX10 antibody
- SOX10_HUMAN antibody
- SRY (sex determining region Y) box 10 antibody
- SRY (sex determining region Y) box 10 antibody
- SRY box 10 antibody
- SRY box containing gene 10 antibody
- SRY related HMG box gene 10 antibody
- SRY related HMG box gene 10 antibody
- Transcription factor SOX 10 antibody
- Transcription factor SOX-10 antibody
- WS2E antibody
- WS4 antibody
- WS4C antibody
see all

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling SOX10 with ab227680 at 1/100 dilution (0.97 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Mainly nuclear staining on the human melanoma, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227680 for 10 mins at room temperature.
Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)

Immunocytochemistry/ Immunofluorescence analysis of C6 (rat glial tumor glial cell) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Rat breast tissue sections labeling SOX10 with ab227680 at 1/100 dilution (0.97 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the rat breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227680 for 10 mins at room temperature.
Flow Cytometry - Anti-SOX10 antibody [SP267] (ab227680)

Flow Cytometry analysis of C6(Rat glial tumor glial cell) cells labeling SOX10 with purified ab227680 at 1:20 dilution (3.75 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Mouse breast tissue sections labeling SOX10 with ab227680 at 1/100 dilution (0.97 μg/ml). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 6.0, epitope retrieval solution 1) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Hematoxylin was used as a counterstain. Nuclear staining on the mouse breast, performed on a Leica Biosystems BOND™ RX instrument.
The section was incubated with ab227680 for 10 mins at room temperature.
Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)

Immunocytochemistry/ Immunofluorescence analysis of B16-F0 ( mouse melanoma epithelial cell-like) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Flow Cytometry - Anti-SOX10 antibody [SP267] (ab227680)

Flow Cytometry analysis of B16-F0(Mouse melanoma epithelial cell-like) cells labeling SOX10 with purified ab227680 at 1:200 dilution (0.375 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Flow Cytometry - Anti-SOX10 antibody [SP267] (ab227680)

Flow Cytometry analysis of A375(Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227680 at 1:200 dilution (0.375 µg/ml) Red. Cells were fixed with 4% paraformaldehyde . A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / Blue.
Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP267] (ab227680)

Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227680 at 1:25 (3 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
Western blot - Anti-SOX10 antibody [SP267] (ab227680)

Anti-SOX10 antibody [SP267] (ab227680) at 1/400 dilution + A-375 (human malignant melanoma cell line) cell lysate

Predicted band size: 49 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP267] (ab227680)

Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227680 at 1/100 dilution in immunohistochemical analysis.

Datasheet

ab227680 has not yet been referenced specifically in any publications.

Publishing research using ab227680? Please let us know so that we can cite the reference in this datasheet.

Location selector

Your location is currently set to:

Custom solutions & partnerships

Recombinant Anti-SOX10 antibody [SP267] (ab227680)

Overview

Properties

Associated products

Applications

Target

Images

Datasheets and documents

References

Customer reviews and Q&As