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    sox10-antibody-sp275-ab227684.pdf

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RecombinantRabMAb

Recombinant Anti-SOX10 antibody [SP275] (ab227684)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
  • Flow Cytometry - Anti-SOX10 antibody [SP275] (ab227684)
  • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (ab227684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
  • Western blot - Anti-SOX10 antibody [SP275] (ab227684)
  • Flow Cytometry - Anti-SOX10 antibody [SP275] (ab227684)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
  • Anti-SOX10 antibody [SP275] (ab227684)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [SP275] to SOX10
  • Suitable for: WB, Flow Cyt, IHC-P, ICC/IF
  • Reacts with: Human

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Overview

  • Product name

    Anti-SOX10 antibody [SP275]
    See all SOX10 primary antibodies
  • Description

    Rabbit monoclonal [SP275] to SOX10
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, Flow Cyt, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.

  • Positive control

    • IHC-P: Human melanoma, and breast tissue; WB: A-375 cell lysate; Flow Cyt: A-375 cells.
  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    This product is FOR RESEARCH USE ONLY. For commercial use, please contact partnerships@abcam.com.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.60
    Preservative: 0.1% Sodium azide
    Constituents: PBS, 1% BSA
  • Concentration information loading...
  • Purity

    Protein A/G purified
  • Purification notes

    Purified from TCS by protein A/G.
  • Clonality

    Monoclonal
  • Clone number

    SP275
  • Isotype

    IgG
  • Research areas

    • Neuroscience
    • Cell Type Marker
    • Neural Stem Cell marker
    • Epigenetics and Nuclear Signaling
    • Transcription
    • Domain Families
    • HMG Box
    • Stem Cells
    • Lineage Markers
    • Ectoderm
    • Developmental Biology
    • Lineage specification
    • Ectoderm
    • Neuroscience
    • Development

Associated products

  • Alternative Versions

    • Anti-SOX10 antibody [SP275] - BSA and Azide free (ab239751)
  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730)
  • Recombinant Protein

    • Recombinant Human SOX10 protein (ab114238)
  • Related Products

    • Anti-PRAME antibody [EPR20330] (ab219650)
    • Anti-PRAME antibody [EPR20330] - BSA and Azide free (ab232571)

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab227684 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
1/400. Predicted molecular weight: 50 kDa.
Flow Cyt
1/400.
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

ICC/IF
1/50.
Notes
WB
1/400. Predicted molecular weight: 50 kDa.
Flow Cyt
1/400.
IHC-P
1/100.

Perform heat mediated antigen retrieval with EDTA buffer pH 8.0 before commencing with IHC staining protocol.

ICC/IF
1/50.

Target

  • Function

    Transcription factor that seems to function synergistically with the POU domain protein TST-1/OCT6/SCIP. Could confer cell specificity to the function of other transcription factors in developing and mature glia.
  • Tissue specificity

    Expressed in fetal brain and in adult brain, heart, small intestine and colon.
  • Involvement in disease

    Defects in SOX10 are the cause of Waardenburg syndrome type 2E (WS2E) [MIM:611584]. WS2 is a genetically heterogeneous, autosomal dominant disorder characterized by sensorineural deafness, pigmentary disturbances, and absence of dystopia canthorum. The frequency of deafness is higher in WS2 than in WS1.
    Defects in SOX10 are a cause of Waardenburg syndrome type 4C (WS4C) [MIM:613266]; also known as Waardenburg-Shah syndrome. WS4C is characterized by the association of Waardenburg features (depigmentation and deafness) and the absence of enteric ganglia in the distal part of the intestine (Hirschsprung disease).
    Defects in SOX10 are a cause of Yemenite deaf-blind hypopigmentation syndrome (YDBHS) [MIM:601706]. YDBHS consists of cutaneous hypopigmented and hyperpigmented spots and patches, microcornea, coloboma and severe hearing loss. Another case observed in a girl with similar skin symptoms and hearing loss but without microcornea or coloboma is reported as a mild form of this syndrome.
    Defects in SOX10 are the cause of peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease (PCWH) [MIM:609136]; also called neurologic variant of Waardenburg-Shah syndrome. PCWH is a rare, complex and more severe neurocristopathy that includes features of 4 distinct syndromes: peripheral demyelinating neuropathy, central dysmyelinating leukodystrophy, Waardenburg syndrome, and Hirschsprung disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Cytoplasm. Nucleus.
  • Target information above from: UniProt accession P56693 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 6663 Human
    • Omim: 602229 Human
    • SwissProt: P56693 Human
    • Unigene: 376984 Human
    • Alternative names

      • DOM antibody
      • DOM antibody
      • Dominant megacolon mouse human homolog of antibody
      • MGC15649 antibody
      • PCWH antibody
      • SOX 10 antibody
      • SOX10 antibody
      • SOX10_HUMAN antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY (sex determining region Y) box 10 antibody
      • SRY box 10 antibody
      • SRY box containing gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • SRY related HMG box gene 10 antibody
      • Transcription factor SOX 10 antibody
      • Transcription factor SOX-10 antibody
      • WS2E antibody
      • WS4 antibody
      • WS4C antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of Human melanoma tissue sections labeling SOX10 with ab227684 at 1/100 dilution (4.09 µg/ml). Heat mediated antigen retrieval was performed Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0, epitope retrieval solution 2) for 10 mins. Rabbit specific IHC polymer detection kit HRP/DAB (ab209101) was used as the secondary antibody. Negative control: PBS instead of the primary antibody. Hematoxylin was used as a counterstain.
    • Flow Cytometry - Anti-SOX10 antibody [SP275] (ab227684)
      Flow Cytometry - Anti-SOX10 antibody [SP275] (ab227684)
      Flow Cytometry analysis of A375 (Human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1:400 dilution (1.02 µg/ml) (red). Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. A Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) secondary antibody was used at 1:2000 dilution. Isotype control - Rabbit monoclonal IgG (ab172730) / Black. Unlabeled control - Unlabelled cells / blue.
    • Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (ab227684)
      Immunocytochemistry/ Immunofluorescence - Anti-SOX10 antibody [SP275] (ab227684)

      Immunocytochemistry/ Immunofluorescence analysis of A375 (human malignant melanoma epithelial cell) cells labeling SOX10 with purified ab227684 at 1:50 (8.1 µg/ml). Cells were fixed in 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) 1:200 (2.5 µg/ml). Goat anti rabbit IgG (Alexa Fluor® 488, ab150077) was used as the secondary antibody at 1:1000 (2 µg/ml) dilution. DAPI (blue) was used as nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)

      Formalin-fixed, paraffin-embedded human melanoma tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

    • Western blot - Anti-SOX10 antibody [SP275] (ab227684)
      Western blot - Anti-SOX10 antibody [SP275] (ab227684)
      Anti-SOX10 antibody [SP275] (ab227684) at 1/400 dilution + A-375 (human malignant melanoma cell line) cell lysate

      Predicted band size: 50 kDa

    • Flow Cytometry - Anti-SOX10 antibody [SP275] (ab227684)
      Flow Cytometry - Anti-SOX10 antibody [SP275] (ab227684)

      Flow Cytometry analysis of A-375 (human malignant melanoma cell line) cells labeling SOX10 with ab227684 at 1/400 dilution (green) compared to a Rabbit IgG negative control (blue).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX10 antibody [SP275] (ab227684)

      Formalin-fixed, paraffin-embedded human breast tissue stained for SOX10 using ab227684 at 1/100 dilution in immunohistochemical analysis.

    • Anti-SOX10 antibody [SP275] (ab227684)
      Anti-SOX10 antibody [SP275] (ab227684)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (1)

    Publishing research using ab227684? Please let us know so that we can cite the reference in this datasheet.

    ab227684 has been referenced in 1 publication.

    • Obeidin F  et al. Sinonasal glomangiopericytoma: A clinicopathologic study. Pathol Res Pract 215:983-987 (2019). PubMed: 30739805

    Customer reviews and Q&As

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