Product nameAnti-SOX11 antibody
See all SOX11 primary antibodies
DescriptionRabbit polyclonal to SOX11
Tested applicationsSuitable for: WBmore details
Species reactivityReacts with: Human
Predicted to work with: Rabbit, Horse, Pig, Chimpanzee, Macaque monkey, Gorilla
Synthetic peptide corresponding to Human SOX11 aa 250-350 conjugated to keyhole limpet haemocyanin.
Database link: P35716
- This antibody gave a positive signal in Human Brain Adult and Fetal tissue lysates.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Batches of this product that have a concentration < 1mg/ml may have BSA added as a stabilising agent. If you would like information about the formulation of a specific lot, please contact our scientific support team who will be happy to help.
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab134739 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).|
FunctionProbably important in the developing nervous system. May also have a role in tissue modeling during development.
Tissue specificityExpressed mainly in the nervous system.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- MRD27 antibody
- SOX11 antibody
- SOX11_HUMAN antibody
All lanes : Anti-SOX11 antibody (ab134739) at 1 µg/ml
Lane 1 : Human brain tissue lysate - total protein (ab29466)
Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)
Lysates/proteins at 10 µg per lane.
All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 47 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Additional bands at: 85 kDa (possible non-specific binding)
Exposure time: 150 seconds
The predicted molecular weight of SOX11 is 46kDa (SwissProt), however we expect to observe a banding pattern around 70kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.
This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134739 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.
ab134739 has not yet been referenced specifically in any publications.