Overview

  • Product name
  • Description
    Rabbit polyclonal to SOX11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: WBmore details
  • Species reactivity
    Reacts with: Human
    Predicted to work with: Rabbit, Horse, Pig, Chimpanzee, Macaque monkey, Gorilla
  • Immunogen

    Synthetic peptide corresponding to Human SOX11 aa 250-350 conjugated to Keyhole Limpet Haemocyanin (KLH).
    Database link: P35716

  • Positive control
    • This antibody gave a positive signal in Human Brain Adult and Fetal tissue lysates.

Properties

Applications

Our Abpromise guarantee covers the use of ab134739 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 70 kDa (predicted molecular weight: 47 kDa).

Target

  • Function
    Probably important in the developing nervous system. May also have a role in tissue modeling during development.
  • Tissue specificity
    Expressed mainly in the nervous system.
  • Sequence similarities
    Contains 1 HMG box DNA-binding domain.
  • Cellular localization
    Nucleus.
  • Information by UniProt
  • Database links
  • Alternative names
    • MRD27 antibody
    • SOX11 antibody
    • SOX11_HUMAN antibody
    • SRY (sex determining region Y) box 11 antibody
    • SRY related HMG box gene 11 antibody
    • SRY-box 11 antibody
    • Transcription factor SOX-11 antibody
    see all

Images

  • All lanes : Anti-SOX11 antibody (ab134739) at 1 µg/ml

    Lane 1 : Human brain tissue lysate - total protein (ab29466)
    Lane 2 : Brain (Human) Tissue Lysate - fetal normal tissue (ab29467)

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 47 kDa
    Observed band size: 70 kDa (why is the actual band size different from the predicted?)
    Additional bands at: 85 kDa (possible non-specific binding)


    Exposure time: 150 seconds


    The predicted molecular weight of SOX11 is 46kDa (SwissProt), however we expect to observe a banding pattern around 70kDa. Abcam welcomes customer feedback and would appreciate any comments regarding this product and the data presented above.

    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 5% Bovine Serum Albumin before being incubated with ab134739 overnight at 4°C. Antibody binding was detected using an anti-rabbit antibody conjugated to HRP, and visualised using ECL development solution.

References

ab134739 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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