Key features and details
- Mouse monoclonal [CL0142] to SOX11
- Suitable for: ICC/IF, IHC-P, WB
- Reacts with: Mouse, Human
- Isotype: IgG2a
Product nameAnti-SOX11 antibody [CL0142]
See all SOX11 primary antibodies
DescriptionMouse monoclonal [CL0142] to SOX11
Tested applicationsSuitable for: ICC/IF, IHC-P, WBmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Pig
- IHC-P: Human mantle cell lymphoma, mouse retina, and mouse brain tissues. WB: SOX11 over-expression HEK293T cell lysate. ICC/IF: RH-30 cells.
General notesThe antibody solution should be gently mixed before use.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles.
Storage bufferpH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab154138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100.
|IHC-P||1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||Use a concentration of 1 µg/ml. Predicted molecular weight: 47 kDa.|
FunctionProbably important in the developing nervous system. May also have a role in tissue modeling during development.
Tissue specificityExpressed mainly in the nervous system.
Sequence similaritiesContains 1 HMG box DNA-binding domain.
- Information by UniProt
- MRD27 antibody
- SOX11 antibody
- SOX11_HUMAN antibody
All lanes : Anti-SOX11 antibody [CL0142] (ab154138) at 1/1000 dilution
Lane 1 : Negative control (vector only transfected HEK293T lysate)
Lane 2 : SOX11 over-expression HEK293T cell lysate (co-expressed with a C terminal myc-DDK tag (~3.1 kDa))
Predicted band size: 47 kDa
Immunohistochemical anaysis of human mantle cell lymphoma labeling SOX11 with ab154138 at 1/200 dilution.
Shows moderate to strong nuclear positivity in tumor cells.
Immunohistochemical anaysis of human chronic lymphoid leukemia tissue showing no labeling of SOX11 with ab154138 at 1/200 dilution (negative control).
Shows no nuclear positivity in tumor cells as expected.
Immunocytochemistry/Immunofluorescence analysis of RH-30 cells labelling SOX11 with ab154138 at 2µg/ml.
Shows specific staining in the nucleus and cytosol in green. Microtubule are visualized in red.
Immunohistochemical anaysis of mouse embryo e14 retina labeling SOX11 with ab154138 at 1/200 dilution.
Shows moderate nuclear positivity in developing retina.
Immunohistochemical anaysis of mouse embryo e14 labeling SOX11 with ab154138 at 1/200 dilution.
Shows strong nuclear positivity in the developing forebrain.
ab154138 has been referenced in 1 publication.
- Chang L et al. miR-145 targets theSOX113'UTR to suppress endometrial cancer growth. Am J Cancer Res 7:2305-2317 (2017). PubMed: 29218252