Anti-SOX11 antibody [CL0142] (ab154138)
Key features and details
- Mouse monoclonal [CL0142] to SOX11
- Suitable for: ICC/IF, IHC-P
- Reacts with: Mouse, Human
- Isotype: IgG2a
Overview
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Product name
Anti-SOX11 antibody [CL0142]
See all SOX11 primary antibodies -
Description
Mouse monoclonal [CL0142] to SOX11 -
Host species
Mouse -
Tested applications
Suitable for: ICC/IF, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Pig -
Immunogen
Recombinant fragment corresponding to amino acids 56-166 of Human SOX11.
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Positive control
- IHC-P: Human mantle cell lymphoma, mouse retina, and mouse brain tissues. ICC/IF: RH-30 cells.
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General notes
The antibody solution should be gently mixed before use.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 59% PBS, 40% Glycerol -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
CL0142 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab154138 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
---|---|---|
ICC/IF |
Use a concentration of 2 - 10 µg/ml.
Fixation/Permeabilization: PFA/Triton X-100. |
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IHC-P |
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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Notes |
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ICC/IF
Use a concentration of 2 - 10 µg/ml. Fixation/Permeabilization: PFA/Triton X-100. |
IHC-P
1/200 - 1/500. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Probably important in the developing nervous system. May also have a role in tissue modeling during development. -
Tissue specificity
Expressed mainly in the nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6664 Human
- Entrez Gene: 20666 Mouse
- Omim: 600898 Human
- SwissProt: P35716 Human
- SwissProt: Q7M6Y2 Mouse
- Unigene: 432638 Human
- Unigene: 41702 Mouse
- Unigene: 466344 Mouse
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Alternative names
- MRD27 antibody
- SOX11 antibody
- SOX11_HUMAN antibody
see all
Images
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Immunohistochemical anaysis of human mantle cell lymphoma labeling SOX11 with ab154138 at 1/200 dilution.
Shows moderate to strong nuclear positivity in tumor cells.
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Immunohistochemical anaysis of human chronic lymphoid leukemia tissue showing no labeling of SOX11 with ab154138 at 1/200 dilution (negative control).
Shows no nuclear positivity in tumor cells as expected.
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Immunocytochemistry/Immunofluorescence analysis of RH-30 cells labelling SOX11 with ab154138 at 2µg/ml.
Shows specific staining in the nucleus and cytosol in green. Microtubule are visualized in red.
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Immunohistochemical anaysis of mouse embryo e14 retina labeling SOX11 with ab154138 at 1/200 dilution.
Shows moderate nuclear positivity in developing retina.
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Immunohistochemical anaysis of mouse embryo e14 labeling SOX11 with ab154138 at 1/200 dilution.
Shows strong nuclear positivity in the developing forebrain.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (2)
ab154138 has been referenced in 2 publications.
- Hussein D et al. A paediatric dysembryoplastic neuroepithelial tumour (DNET) with deregulated stem cell markers: a case report. Transl Pediatr 11:1040-1049 (2022). PubMed: 35800288
- Chang L et al. miR-145 targets theSOX113'UTR to suppress endometrial cancer growth. Am J Cancer Res 7:2305-2317 (2017). PubMed: 29218252