Recombinant Anti-SOX11 antibody [EPR20457] (ab229185)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR20457] to SOX11
- Suitable for: WB, IHC-P
- Reacts with: Human
Related conjugates and formulations
Overview
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Product name
Anti-SOX11 antibody [EPR20457]
See all SOX11 primary antibodies -
Description
Rabbit monoclonal [EPR20457] to SOX11 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- WB: SH-SY5Y whole cell lysate; Human fetal brain lysate. IHC-P: Human mantle cell lymphoma and glioma tissues.
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General notes
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.2
Preservative: 0.01% Sodium azide
Constituents: PBS, 40% Glycerol (glycerin, glycerine), 0.05% BSA -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR20457 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Isotype control
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab229185 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
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IHC-P |
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
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Notes |
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WB
1/1000. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa). |
IHC-P
1/250. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol. |
Target
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Function
Probably important in the developing nervous system. May also have a role in tissue modeling during development. -
Tissue specificity
Expressed mainly in the nervous system. -
Sequence similarities
Contains 1 HMG box DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 6664 Human
- Omim: 600898 Human
- SwissProt: P35716 Human
- Unigene: 432638 Human
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Alternative names
- MRD27 antibody
- SOX11 antibody
- SOX11_HUMAN antibody
see all
Images
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All lanes : Anti-SOX11 antibody [EPR20457] (ab229185) at 1/1000 dilution
Lane 1 : SH-SY5Y (human neuroblastoma cell line from bone marrow) whole cell lysate at 20 µg
Lane 2 : Human fetal brain lysate at 10 µg
Secondary
All lanes : VeriBlot for IP Detection Reagent (HRP) (ab131366) at 1/1000 dilution
Predicted band size: 47 kDaExposure time : Lane 1: 1 minute, Lane 2: 3 minutes.
Blocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is phosphorylated form, consistent with what has been described in the literature. (PMID:19880779, PMID:29973868).
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Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma tissue labeling SOX11 with ab229185 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining in human mantle cell lymphoma (PMID: 19880788. PMID:17934069) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
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Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling SOX11 with ab229185 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining on human glioma (PMID:15583815.) is observed. Counterstained with hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.
Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
Certificate of Compliance
References (1)
ab229185 has been referenced in 1 publication.
- Ma Z et al. MiR-937-3p promotes metastasis and angiogenesis and is activated by MYC in lung adenocarcinoma. Cancer Cell Int 22:31 (2022). PubMed: 35033084