Recombinant
RabMAb

Recombinant Anti-SOX11 antibody [EPR20457] - BSA and Azide free (ab239787)

Overview

  • Product name

    Anti-SOX11 antibody [EPR20457] - BSA and Azide free
    See all SOX11 primary antibodies
  • Description

    Rabbit monoclonal [EPR20457] to SOX11 - BSA and Azide free
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SOX11 aa 1-100. The exact sequence is proprietary.
    Database link: P35716

  • Positive control

    • IHC-P: Human mantle cell lymphoma tissue.
  • General notes

    Ab239787 is the carrier-free version of ab229185. This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    ab239787 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab239787 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use at an assay dependent concentration. Detects a band of approximately 60 kDa (predicted molecular weight: 47 kDa).
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

Target

  • Function

    Probably important in the developing nervous system. May also have a role in tissue modeling during development.
  • Tissue specificity

    Expressed mainly in the nervous system.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • MRD27 antibody
    • SOX11 antibody
    • SOX11_HUMAN antibody
    • SRY (sex determining region Y) box 11 antibody
    • SRY related HMG box gene 11 antibody
    • SRY-box 11 antibody
    • Transcription factor SOX-11 antibody
    see all

Images

  • Immunohistochemical analysis of paraffin-embedded human glioma tissue labeling SOX11 with ab229185 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining on human glioma (PMID:15583815.) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab229185).

  • Immunohistochemical analysis of paraffin-embedded human mantle cell lymphoma tissue labeling SOX11 with ab229185 at 1/250 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) Ready to use. Nucleus staining in human mantle cell lymphoma (PMID: 19880788. PMID:17934069) is observed. Counterstained with hematoxylin.

    Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) Ready to use.

    Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

    This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol and sodium azide (ab229185).

     

References

ab239787 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab239787.
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Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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