Overview

  • Product name
    Anti-SOX11 antibody [EPR8191(2)]
    See all SOX11 primary antibodies
  • Description
    Rabbit monoclonal [EPR8191(2)] to SOX11
  • Host species
    Rabbit
  • Tested applications
    Suitable for: ICC/IF, Flow Cyt, IP, WBmore details
    Unsuitable for: IHC
  • Species reactivity
    Reacts with: Mouse, Rat, Human
  • Immunogen

    Synthetic peptide within Human SOX11 aa 1-100 (N terminal) (Cysteine residue). The exact sequence is proprietary.
    Database link: P35716

  • Positive control
    • WB: Human fetal brain tissue lysate and Y79 cell lysate. ICC/IF: SH-SY5Y cells. Flow Cyt: SH-SY5Y cells.
  • General notes

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab170916 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF 1/50 - 1/100.
Flow Cyt 1/60.
IP 1/10 - 1/100.
WB 1/1000 - 1/10000. Predicted molecular weight: 47 kDa.
  • Application notes
    Is unsuitable for IHC.
  • Target

    Images

    • All lanes : Anti-SOX11 antibody [EPR8191(2)] (ab170916) at 1/1000 dilution

      Lane 1 : Human fetal brain tissue lysate
      Lane 2 : SH SY5Y cell lysate
      Lane 3 : Y79 cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat anti-rabbit HRP conjugated antibody at 1/2000 dilution

      Developed using the ECL technique.

      Predicted band size: 47 kDa

    • Immunofluorescent analysis of SH SY5Y cells labeling SOX11 with ab170916 at 1/50.

    • Flow cytometry analysis of SH-SY5Y ( Human neuroblastoma epithelial cell) cells labeling SOX11 (red) with ab170916 at a 1/60 dilution. Cells were fixed with 4% paraformaldehyde and permeabilized with 90% methanol. A goat anti-rabbit IgG (Alexa Fluor® 488) (ab150077) was used as the secondary antibody at a 1/2000 dilution. Black - Rabbit monoclonal IgG (Black) (ab172730). Blue (unlabeled control) - Cell without incubation with primary antibody and secondary antibody (Blue).

    • Lane 1 Primary: ab170916, 1/1000 dilution
      Sample: Immunoprecipitation pellet from Human fetal brain lysate using ab170916 at 1/10.
      Secondary: HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit IgG.

      Lane 2 Primary: ab170916, 1/1000 dilution
      Sample: Immunoprecipitation pellet from PBS using ab170916 at 1/10 (negative control).
      Secondary: HRP-conjugated anti-rabbit IgG preferentially detecting the non-reduced form of rabbit

    References

    This product has been referenced in:
    See 1 Publication for this product

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    Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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