Overview

  • Product name

    Anti-SOX17 antibody [BC24-3.5CH]
    See all SOX17 primary antibodies
  • Description

    Mouse monoclonal [BC24-3.5CH] to SOX17
  • Host species

    Mouse
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Dog, Human
  • Positive control

    • IHC-P: Mouse embryo tissue, endothelial cells and gut endoderm. ICC/IF: Rh18 and Rh30 cells, TF1 hematopoietic stem cell line. WB: MDCK cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab192453 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 2 - 3 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 3 - 5 µg/ml.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells.
  • Tissue specificity

    Expressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.
  • Involvement in disease

    Defects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3) [MIM:613674]. VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
    Contains 1 Sox C-terminal domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ22252 antibody
    • SOX17 antibody
    • SOX17_HUMAN antibody
    • SRY (sex determining region Y) box 17 antibody
    • SRY box 17 antibody
    • SRY related HMG box transcription factor SOX17 antibody
    • Transcription factor SOX-17 antibody
    • Transcription factor SOX17 antibody
    • VUR3 antibody
    see all

Images

  • Anti-SOX17 antibody [BC24-3.5CH] (ab192453) at 2 µg/ml + MDCK (Canine kidney cell line) cell lysate at 20 µg

    Secondary
    Peroxidase conjugated rabbit anti-mouse Ig at 1/20000 dilution

    Predicted band size: 45 kDa

  • Immunofluorescence staining of Rh18 and Rh30 Cells after treatment with Aphidicolin or Nocadazole labeling SOX17 using ab192453 and a PE anti-mouse Ig labeled secondary antibody.

  • Immunohistochemical analysis of PFA-fixed mouse embryo (A), gut endoderm (B) and endothelial cells (C), labeling SOX17 using ab192453 at 5 µg/mL, followed by a peroxidase labeled anti-mouse Ig secondary antibody and DAB staining.

  • Immunofluorescence analysis of endothelial cells and gut endoderm, staining SOX17 using ab192453 and a TRITC labeled anti mouse Ig secondary antibody. 

  • Immunofluorescence staining of TF1 hematopoietic stem cell line labeling SOX17 using ab192453 and a TRITC labeled anti-mouse Ig secondary antibody.

References

This product has been referenced in:

  • Chaturvedi P  et al. Stable and reproducible transgene expression independent of proliferative or differentiated state using BAC TG-EMBED. Gene Ther N/A:N/A (2018). Read more (PubMed: 29930343) »
  • Wu H  et al. Retinoic acid-induced upregulation of miR-219 promotes the differentiation of embryonic stem cells into neural cells. Cell Death Dis 8:e2953 (2017). Read more (PubMed: 28749472) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (Testis, adult)
Permeabilization
Yes - 0.1% Triton X-100 in PBS
Specification
Testis, adult
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 3% · Temperature: 20°C
Fixative
Paraformaldehyde

Mr. Bryan Niedenberger

Verified customer

Submitted Jul 05 2017

Application
Western blot
Sample
Human Cell lysate - nuclear (seminoma)
Gel Running Conditions
Reduced Denaturing (Bolt Bis-Tris 4-12% SDSPAGE)
Loading amount
156000 cells
Specification
seminoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 12 2017

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