Recombinant
RabMAb

Recombinant Anti-SOX17 antibody [EPR20684] (ab224637)

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Overview

  • Product name

    Anti-SOX17 antibody [EPR20684]
    See all SOX17 primary antibodies

  • Description

    Rabbit monoclonal [EPR20684] to SOX17

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, IP, WBmore details

  • Species reactivity

    Reacts with: Mouse, Rat, Human

  • Immunogen

    Synthetic peptide within Human SOX17 aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: Q9H6I2

  • Positive control

    • WB: SOX17 recombinant protein. IHC-P: Human seminoma tissue; Rat lung tissue; Mouse spleen tissue. IP: SK-OV-3 whole cell lysate.

  • General notes

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

Properties

Applications

Our Abpromise guarantee covers the use of ab224637 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P 1/2000. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
IP 1/30.
WB 1/500. Detects a band of approximately 55 kDa (predicted molecular weight: 44 kDa).

Target

  • Function

    Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells.

  • Tissue specificity

    Expressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.

  • Involvement in disease

    Defects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3) [MIM:613674]. VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.

  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
    Contains 1 Sox C-terminal domain.

  • Cellular localization

    Nucleus.
  • Information by UniProt

  • Database links

    • Alternative names

      • FLJ22252 antibody
      • SOX17 antibody
      • SOX17_HUMAN antibody
      • SRY (sex determining region Y) box 17 antibody
      • SRY box 17 antibody
      • SRY related HMG box transcription factor SOX17 antibody
      • Transcription factor SOX-17 antibody
      • Transcription factor SOX17 antibody
      • VUR3 antibody
      see all

    Images

    • Western blot - Anti-SOX17 antibody [EPR20684] (ab224637)
      Western blot - Anti-SOX17 antibody [EPR20684] (ab224637)
      All lanes : Anti-SOX17 antibody [EPR20684] (ab224637) at 1/500 dilution

      Lane 1 : SK-OV-3 (Human adenocarcinoma) whole cell lysates with NFDM/TBST
      Lane 2 : NIH: OVCAR-3 (Human ovary adenocarcinoma) whole cell lysates with NFDM/TBST
      Lane 3 : HeLa (Human cervix adenocarcinoma) whole cell lysates with NFDM/TBST
      Lane 4 : Rat E14 embryo tissue lysates with NFDM/TBST
      Lane 5 : Rat E9.5 embryo tissue lysates with NFDM/TBST
      Lane 6 : Rat lung tissue lysates with NFDM/TBST

      Lysates/proteins at 20 µg per lane.

      Blocking peptides at 5 % per lane.

      Secondary
      All lanes : Goat Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG) at 1/2000 dilution

      Predicted band size: 44 kDa



      Exposure time: 
      Lanes 1-2: 5 seconds
      Lanes 3-6: 3 minutes

      The expression profile observed is consistent with the literature (PMID: 11786926).

      Negative control: HeLa.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)

      Immunohistochemical analysis of paraffin-embedded human seminoma tissue labeling SOX17 with ab224637 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on tumor cells of human seminoma (PMID:19369635; PMID:18348160) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunoprecipitation - Anti-SOX17 antibody [EPR20684] (ab224637)
      Immunoprecipitation - Anti-SOX17 antibody [EPR20684] (ab224637)

      SOX17 was immunoprecipitated from 0.35 mg of SK-OV-3 (human ovarian cancer cell line) lysate with ab224637 at 1/30 dilution. Western blot was performed from the immunoprecipitate using ab224637 at 1/500 dilution. VeriBlot for IP Detection Reagent (HRP) (ab131366), was used for detection at 1/1000 dilution.

      Lane 1: SK-OV-3 whole cell lysate 10 µg (Input). 

      Lane 2: ab224637 IP in SK-OV-3 whole cell lysate. 

      Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab224637 in SK-OV-3 whole cell lysate.

      Blocking and dilution buffer and concentration: 5% NFDM/TBST.

      Exposure time: 1 second.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)

      Immunohistochemical analysis of paraffin-embedded human choriocarcinoma tissue labeling SOX17 with ab224637 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Negative tissue: no staining on tumor cells of human choriocarcinoma (PMID: 19369635) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)

      Immunohistochemical analysis of paraffin-embedded rat lung tissue labeling SOX17 with ab224637 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelium of rat lung (PMID:24418654) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-SOX17 antibody [EPR20684] (ab224637)

      Immunohistochemical analysis of paraffin-embedded mouse spleen tissue labeling SOX17 with ab224637 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) ready to use. Nuclear staining on endothelium of mouse spleen (PMID:24418654) is observed. Counter stained with Hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) ready to use.

      Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.

    References

    ab224637 has not yet been referenced specifically in any publications.

    Publishing research using ab224637? Please let us know so that we can cite the reference in this datasheet.

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    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX17 antibody [EPR20684]

     Good
    Abreviews
    abreview image
    Application
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
    Sample
    Human Tissue sections (Normal Breast)
    Antigen retrieval step
    Heat mediated - Buffer/Enzyme Used: ab94681 Tris EDTA pH9.0
    Permeabilization
    Yes - 0.05% tween 20
    Specification
    Normal Breast
    Blocking step
    Sea Block as blocking agent for 30 minute(s) · Concentration: 100% · Temperature: 22°C
    Fixative
    10%NBF
    Read More

    Mr. David Ivancic

    Verified customer

    Submitted Jun 05 2018

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