Overview

  • Product name

    Anti-SOX17 antibody [OTI3B10]
    See all SOX17 primary antibodies
  • Description

    Mouse monoclonal [OTI3B10] to SOX17
  • Host species

    Mouse
  • Tested applications

    Suitable for: Flow Cyt, WB, ICC/IFmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Recombinant protein, expressed in E.coli corresponding to amino acids 177-414 of Human SOX17

  • Positive control

    • WB: HepG2, HeLa, SVT2, A549, COS7, Jurkat and MDCK cell lysates. ICC/IF: HT29 cells. Flow Cyt: Transfected HEK293 expressing SOX17.
  • General notes

    This product was changed from ascites to tissue culture supernatant on 31st May 2019. Please note that the dilutions may need to be adjusted accordingly. If you have any questions, please do not hesitate to contact our scientific support team.

    ab84990 should be diluted with PBS before use.

    The clone number has been updated from 3B10 to OTI3B10, both clone numbers name the same clone.

Properties

Applications

Our Abpromise guarantee covers the use of ab84990 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Flow Cyt Use at an assay dependent concentration.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

WB Use at an assay dependent concentration. Predicted molecular weight: 44 kDa.
ICC/IF Use at an assay dependent concentration.

Target

  • Function

    Acts as transcription regulator that binds target promoter DNA and bends the DNA. Binds to the sequences 5'-AACAAT-'3 or 5'-AACAAAG-3'. Modulates transcriptional regulation via WNT3A. Inhibits Wnt signaling. Promotes degradation of activated CTNNB1. Plays a key role in the regulation of embryonic development. Required for normal looping of the embryonic heart tube. Required for normal development of the definitive gut endoderm. Probable transcriptional activator in the premeiotic germ cells.
  • Tissue specificity

    Expressed in adult heart, lung, spleen, testis, ovary, placenta, fetal lung, and kidney. In normal gastrointestinal tract, it is preferentially expressed in esophagus, stomach and small intestine than in colon and rectum.
  • Involvement in disease

    Defects in SOX17 are the cause of vesicoureteral reflux type 3 (VUR3) [MIM:613674]. VUR3 is a disease belonging to the group of congenital anomalies of the kidney and urinary tract. It is characterized by the reflux of urine from the bladder into the ureters and sometimes into the kidneys, and is a risk factor for urinary tract infections. Primary disease results from a developmental defect of the ureterovesical junction. In combination with intrarenal reflux, the resulting inflammatory reaction may result in renal injury or scarring, also called reflux nephropathy. Extensive renal scarring impairs renal function and may predispose patients to hypertension, proteinuria, renal insufficiency and end-stage renal disease.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
    Contains 1 Sox C-terminal domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • FLJ22252 antibody
    • SOX17 antibody
    • SOX17_HUMAN antibody
    • SRY (sex determining region Y) box 17 antibody
    • SRY box 17 antibody
    • SRY related HMG box transcription factor SOX17 antibody
    • Transcription factor SOX-17 antibody
    • Transcription factor SOX17 antibody
    • VUR3 antibody
    see all

Images

  • Immunocytochemistry/Immunofluorescence analysis of HT29 cells labelling SOX17 with ab84990.

    This image was generated using the ascites version of the product.

     

  • All lanes : Anti-SOX17 antibody [OTI3B10] (ab84990) at 1/200 dilution

    Lane 1 : HepG2 cell lysate
    Lane 2 : HeLa cell lysate
    Lane 3 : SVT2 cell lysate
    Lane 4 : A549 cell lysate
    Lane 5 : COS7 cell lysate
    Lane 6 : Jurkat cell lysate
    Lane 7 : MDCK cell lysate

    Lysates/proteins at 10 µg per lane.

    Predicted band size: 44 kDa



    This image was generated using the ascites version of the product.

  • Flow cytometry analysis of HEK293T cells transfected with SOX17 (red) or an empty vector control plasmid (blue) labelling SOX17 with ab84990.

    This image was generated using the ascites version of the product.

References

This product has been referenced in:

  • Sato K  et al. Distribution of label-retaining cells and their properties in the vocal fold mucosa. Laryngoscope Investig Otolaryngol 4:76-82 (2019). Read more (PubMed: 30828622) »
  • Varghese DS  et al. Effects of Aminoglycoside Antibiotics on Human Embryonic Stem Cell Viability during Differentiation In Vitro. Stem Cells Int 2017:2451927 (2017). Read more (PubMed: 29147115) »
See all 7 Publications for this product

Customer reviews and Q&As

1-3 of 3 Abreviews or Q&A

Application
Western blot
Sample
Human Cell lysate - nuclear (seminoma)
Gel Running Conditions
Reduced Denaturing (Bolt Bis-Tris 4-12% SDS PAGE)
Loading amount
156000 cells
Specification
seminoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Apr 11 2017

Application
Immunocytochemistry
Sample
Human Cultured Cells (NPCs derived from iPSC)
Permeabilization
Yes - 0.25% Triton X-100 in PBS
Specification
NPCs derived from iPSC
Blocking step
BSA as blocking agent for 30 minute(s) · Concentration: 1% · Temperature: 22°C
Fixative
Paraformaldehyde

Abcam user community

Verified customer

Submitted Feb 10 2016

Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (iCell® Neurons)
Permeabilization
No
Specification
iCell® Neurons
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Fixative
Acetone

Abcam user community

Verified customer

Submitted Sep 17 2015

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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