Overview

  • Product name

    Anti-SOX18 antibody [EPR2837(2)]
    See all SOX18 primary antibodies
  • Description

    Rabbit monoclonal [EPR2837(2)] to SOX18
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
    Unsuitable for: Flow Cyt,ICC or IP
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide within Human SOX18 aa 300-400. The exact sequence is proprietary.

  • Positive control

    • K562, Human stomach, Jurkat, and HeLa cell lysates
  • General notes

    Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.

     

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.

    We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.

    This product is a recombinant rabbit monoclonal antibody.

Properties

Applications

Our Abpromise guarantee covers the use of ab109194 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).
IHC-P 1/100 - 1/250. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Antigen retrieval is recommended.

  • Application notes
    Is unsuitable for Flow Cyt,ICC or IP.
  • Target

    • Function

      Binds to the consensus sequence 5'-AACAAAG-3' and is able to trans-activate transcription via this site.
    • Involvement in disease

      Defects in SOX18 are the cause of hypotrichosis-lymphedema-telangiectasia syndrome (HLTS) [MIM:607823].
    • Sequence similarities

      Contains 1 HMG box DNA-binding domain.
      Contains 1 Sox C-terminal domain.
    • Cellular localization

      Nucleus.
    • Information by UniProt
    • Database links

    • Alternative names

      • HLTS antibody
      • SOX 18 antibody
      • Sox18 antibody
      • SOX18_HUMAN antibody
      • SRY (sex determining region Y) box 18 antibody
      • SRY box 18 antibody
      • Transcription factor SOX 18 antibody
      • Transcription factor SOX-18 antibody
      see all

    Images

    • All lanes : Anti-SOX18 antibody [EPR2837(2)] (ab109194) at 1/1000 dilution

      Lane 1 : K562 cell lysate
      Lane 2 : Human stomach cell lysate
      Lane 3 : Jurkat cell lysate
      Lane 4 : HeLa cell lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution

      Predicted band size: 41 kDa
      Observed band size: 41 kDa

    References

    This product has been referenced in:

    • Miao Z  et al. Upregulation of SOX18 in colorectal cancer cells promotes proliferation and correlates with colorectal cancer risk. Onco Targets Ther 11:8481-8490 (2018). Read more (PubMed: 30555240) »
    See 1 Publication for this product

    Customer reviews and Q&As

    There are currently no Customer reviews or Questions for ab109194.
    Please use the links above to contact us or submit feedback about this product.

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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