Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR2837(2)] to SOX18
- Suitable for: WB
- Reacts with: Human
Product nameAnti-SOX18 antibody [EPR2837(2)]
See all SOX18 primary antibodies
DescriptionRabbit monoclonal [EPR2837(2)] to SOX18
Tested applicationsSuitable for: WBmore details
Unsuitable for: Flow Cyt,ICC or IP
Species reactivityReacts with: Human
Synthetic peptide within Human SOX18 aa 300-400. The exact sequence is proprietary.
- K562, Human stomach, Jurkat, and HeLa cell lysates
Mouse, Rat: We have preliminary internal testing data to indicate this antibody may not react with these species. Please contact us for more information.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated 'PUR' on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferpH: 7.20
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol (glycerin, glycerine), 0.5% BSA
Concentration information loading...
PurityProtein A purified
Our Abpromise guarantee covers the use of ab109194 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000 - 1/10000. Detects a band of approximately 41 kDa (predicted molecular weight: 41 kDa).|
FunctionBinds to the consensus sequence 5'-AACAAAG-3' and is able to trans-activate transcription via this site.
Involvement in diseaseDefects in SOX18 are the cause of hypotrichosis-lymphedema-telangiectasia syndrome (HLTS) [MIM:607823].
Sequence similaritiesContains 1 HMG box DNA-binding domain.
Contains 1 Sox C-terminal domain.
- Information by UniProt
- HLTS antibody
- SOX 18 antibody
- Sox18 antibody
All lanes : Anti-SOX18 antibody [EPR2837(2)] (ab109194) at 1/1000 dilution
Lane 1 : K562 cell lysate
Lane 2 : Human stomach cell lysate
Lane 3 : Jurkat cell lysate
Lane 4 : HeLa cell lysate
Lysates/proteins at 10 µg per lane.
All lanes : HRP labelled goat anti-rabbit at 1/2000 dilution
Predicted band size: 41 kDa
Observed band size: 41 kDa
ab109194 has been referenced in 2 publications.
- Xu Y et al. Down-regulation of SOX18 inhibits laryngeal carcinoma cell proliferation, migration, and invasion through JAK2/STAT3 signaling. Biosci Rep 39:N/A (2019). PubMed: 31189744
- Miao Z et al. Upregulation of SOX18 in colorectal cancer cells promotes proliferation and correlates with colorectal cancer risk. Onco Targets Ther 11:8481-8490 (2018). PubMed: 30555240