Overview

  • Product name

    Anti-SOX2 antibody [9-9-3]
    See all SOX2 primary antibodies
  • Description

    Mouse monoclonal [9-9-3] to SOX2
  • Host species

    Mouse
  • Tested applications

    Suitable for: ICC/IF, WB, IHC-Fr, Flow Cytmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human, Apteronotus leptorhynchus
    Predicted to work with: Sheep, Horse, Chicken, Cow, Pig, Xenopus laevis, Rhesus monkey
  • Immunogen

    Synthetic peptide corresponding to Human SOX2 aa 300 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab80398)

  • Positive control

    • ICC/IF: NCCIT and F9 cells. WB: F9 and PC-3 whole cell lysate. Flow Cyt: F9 cells.
  • General notes

    This antibody clone is manufactured by Abcam.

    If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.

Properties

Applications

Our Abpromise guarantee covers the use of ab79351 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ICC/IF Use at an assay dependent concentration.
WB Use a concentration of 1 µg/ml. Detects a band of approximately 43 kDa (predicted molecular weight: 34 kDa).
IHC-Fr Use at an assay dependent concentration.
Flow Cyt Use 0.1-1µg for 106 cells.

ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

 

Target

  • Function

    Transcription factor that forms a trimeric complex with OCT4 on DNA and controls the expression of a number of genes involved in embryonic development such as YES1, FGF4, UTF1 and ZFP206 (By similarity). Critical for early embryogenesis and for embryonic stem cell pluripotency.
  • Involvement in disease

    Defects in SOX2 are the cause of microphthalmia syndromic type 3 (MCOPS3) [MIM:206900]. Microphthalmia is a clinically heterogeneous disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues (anophthalmia). In many cases, microphthalmia/anophthalmia occurs in association with syndromes that include non-ocular abnormalities. MCOPS3 is characterized by the rare association of malformations including uni- or bilateral anophthalmia or microphthalmia, and esophageal atresia with trachoesophageal fistula.
  • Sequence similarities

    Contains 1 HMG box DNA-binding domain.
  • Post-translational
    modifications

    Sumoylation inhibits binding on DNA and negatively regulates the FGF4 transactivation.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • ANOP3 antibody
    • cb236 antibody
    • Delta EF2a antibody
    • lcc antibody
    • MCOPS3 antibody
    • MGC148683 antibody
    • MGC2413 antibody
    • RGD1565646 antibody
    • Sex determining region Y box 2 antibody
    • SOX 2 antibody
    • Sox2 antibody
    • SOX2_HUMAN antibody
    • SRY (sex determining region Y) box 2 antibody
    • SRY box containing gene 2 antibody
    • SRY related HMG box 2 antibody
    • SRY related HMG box gene 2 antibody
    • SRY-box 2 antibody
    • Transcription factor SOX 2 antibody
    • Transcription factor SOX-2 antibody
    • ysb antibody
    see all

Images

  • ab79351 staining SOX2 in 16 µm thick sections of Apteronotus leptorhynchus brain tissue by Immunohistochemistry (Frozen sections).
    Tissue was fixed in 2% paraformaldehyde, permeabilized using 0.3% Triton X-100, blocked with 3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS for 1 hour at 24°C and then incubated with ab79351 at a 1/50 dilution for 18 hours at 4°C. The secondary used was an Alexa-Fluor 488 conjugated goat anti-mouse polyclonal used at a 1/200 dilution.

    See Abreview

  • Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NCCIT (human pluripotent embryonal carcinoma) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on NCCIT cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
    The negative controls are as follows:
    -ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
    -ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.

  • All lanes : Anti-SOX2 antibody [9-9-3] (ab79351) at 1/10000 dilution

    Lane 1 : NCCIT whole cell lysate
    Lane 2 : PC-3 whole cell lysate

    Lysates/proteins at 10 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/2000 dilution

    Predicted band size: 34 kDa
    Observed band size: 34 kDa



    Blocking buffer: 5% NFDM/TBST
    Dilution buffer: 5% NFDM/TBST

  • Overlay histogram showing F9 cells stained with ab79351 (red line). The cells were fixed with 4% paraformaldehyde (10 min) and incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab79351, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was a goat anti-mouse DyLight® 488 (IgG H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive result in 80% methanol (5 min) fixed F9 cells used under the same conditions.

    Please note that Abcam do not have any data for use of this antibody on non-fixed cells. We welcome any customer feedback.

  • Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized F9 (mouse embryonal carcinoma) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing positive staining on F9 cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
    The negative controls are as follows:
    -ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
    -ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.

     

     

  • ICC/IF image of ab79351 stained D3 mouse embryonic stem cells. The cells were fixed in 4% Paraformaldehyde, permeabilized using 0.1% Triton X-100, blocked with 1% Goat serum, 0.1% BSA in PBS for 30 minutes at RT, before incubation with ab79351 at a 1/100 dilution for 2 hours at RT. The secondary used was an Alexa Fluor 488 conjugated goat anti-mouse polyclonal, used at 1/200 dilution.

    See Abreview

  • Immunofluorescent analysis of 4% PFA-fixed, 0.1% Triton X-100 permeabilized NIH/3T3 (mouse embryonic fibroblast cell line) (Negative control) cells labeling SOX2 with ab79351 at 1/200 dilution, followed by ab150113 AlexaFluor®488 Goat anti-Mouse secondary antibody at 1/1000 dilution (green). Confocal image showing showing no staining on NIH/3T3 cells. Counterstained with ab179504 anti-Tubulin (Rabbit mAb, 1/1000), ab150080 AlexaFluor®594 Goat anti-Rabbit secondary at a 1/1000 dilution. Nuclear stained with DAPI.
    The negative controls are as follows:
    -ve control 1: ab79351 at 1/200 dilution, followed by ab150080 at 1/1000 dilution.
    -ve control 2: ab179504 at 1/1000 dilution, followed by ab150113 at 1/1000 dilution.

References

This product has been referenced in:

See all 24 Publications for this product

Customer reviews and Q&As

1-2 of 2 Q&A

Answer

Vielen Dank fürIhre Anfrage.

Leider haben wirH00006657-A01 derzeit nicht in unserem Katalog verfügbar. Allerdings kann ich Ihnen gerne drei unserer anti-SOX2- Antikörper empfehlen, die wir für dieImmunhistochemie an humanen Paraffinschnitten (ICH-P) garantieren können:

ab97959: ein Kaninchen-polyklonaler anti-SOX2-Antikörper
https://www.abcam.com/index.html?datasheet=97959 (or use the following: https://www.abcam.com/index.html?datasheet=97959).

ab79351: ein Maus-monoklonaleranti-SOX2-Antikörper
https://www.abcam.com/index.html?datasheet=79351 (or use the following: https://www.abcam.com/index.html?datasheet=79351).

ab110145: ein Ziegen-polyklonaler anti-SOX2-Antikörper
https://www.abcam.com/index.html?datasheet=110145 (or use the following: https://www.abcam.com/index.html?datasheet=110145).

Wie alle unsere Produkte unterliegen diese Antikörper auch unserer Abcam-Garantie: Wenn sie nicht so funktionieren, wie auf dem Datenblatt beschrieben, gibt es einen kostenlosen Ersatz oder Ihr Geld zurück (https://www.abcam.com/Abpromise).

Ich hoffe, dies hilft Ihnen weiter. Bitte zögern Sie nicht, sich wieder bei uns zu melden, falls Sie weitere Fragen haben.

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Question
Answer

Thank you for your call today, and I apologize for the trouble with these codes! I have created new codes which will expire on March 1, 2012. I will go through your Abreviews and put these new codes in our internal notes so that the discounts can be applied to your orders tomorrow. Please let me know if you have any questions, or if there is anything else that we can do for you.

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