Immunohistochemistry (Frozen sections) abreview for Anti-SOX2 antibody [9-9-3]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Apteronotus leptorhynchus Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Permeabilization
Yes - 0.3% Triton X-100
Blocking step
3% sheep serum, 1% BSA, 1% teleostean gelatine in TBS as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 24°C

Other product details

Dilution
1/50
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: blocking solution

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor 488-conjugated goat anti-mouse IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

Additional data

Additional Notes
The antibody was applied on 16 µm thick sections of brown ghost knifefish spinal cord and brain. Excellent nuclear labeling of numerous cells in the ventricular regions of the intact brain.

Dr. Ruxandra Sirbulescu

Verified customer

Submitted Nov 14 2011

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