Western blot abreview for Anti-SOX2 antibody [9-9-3]

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - whole cell (D3 Mouse Embryonic Stem Cell Line)
Loading amount
200000 cells
Specification
D3 Mouse Embryonic Stem Cell Line
Gel Running Conditions
Reduced Denaturing (gel 10%)
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: RT°C

Other product details

Concentration
0.5 µg/ml
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk

Secondary antibody

Name
Non-Abcam antibody was used: Dako Polyclonal Goat anti-Mouse Immunoglobulin/HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000

Detection

Detection method
Lumigen TMA-6
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: ~32 kDa
Positive control
D3 (Mouse Embryonic Stem Cell Line) whole cell lysate. Sox2 is expressed in pluripotent embryonic stem cells.

Additional data

Additional Notes
Lane 1 - Sox2 antibody used at 0.5 ug/ml.
Lane 2 - Sox2 antibody used at 1.0 ug/ml.

Joe Segal

Verified customer

Submitted Sep 04 2012

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