Immunohistochemistry (Frozen sections) abreview for Anti-SOX2 antibody [9-9-3]

Excellent
Abreviews
Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (12µm frozen sections of mouse cerebellum)
Specification
12µm frozen sections of mouse cerebellum
Fixative
Paraformaldehyde
Permeabilization
No
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: RT°C

Other product details

Dilution
1/50
Incubation time
20 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Antibody Diluent (Ventana)

Secondary antibody

Name
Non-Abcam antibody was used: Invitrogen goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/500

Additional data

Additional Notes
Image taken of test at primary antibody dilution of 1:50, but 1:200 works as well.

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Submitted Jan 04 2013

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