Western blot abreview for Anti-SOX2 antibody [9-9-3]

Excellent
Abreviews
Application
Western blot
Loading amount
100 µg
Gel Running Conditions
Reduced Denaturing (4-12% Nupage, MOPS)
Sample
Mouse Cell lysate - whole cell (Embryonic stem cells (E14Tg2a))
Specification
Embryonic stem cells (E14Tg2a)
Treatment
DMSO or GlcNAcstatin C (Oga inhibitor) for 24h
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in TBST
Dilution
1/1000

Secondary antibody

Dilution
1/1000

Detection

Detection method
LumiGLO
Bands
Specific: 39 kDa
Exposure
45 second(s)
Negative control
Embryonic stem cell lysates mock immunoprecipitated (lane 5).
Positive control
Embryonic stem cell lysates immunoprecipitated with a rabbit anti-Sox2 (ab97959) covalently bound to Sepharose-Protein G beads (lane 1).

Additional data

Additional Notes
1: ES cells + DMSO (0.01%). 2: ES cells + Oga inhibitor (GlcNAcstatin C, 1uM). 3: Differentiated ES cells + DMSO (0.01%). 4: Differentiated ES cells + Oga inhibitor (GlcNAcstatin C, 1uM). 5: ES cells + Oga inhibitor (GlcNAcstatin C, 1uM), Mock IP.

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Submitted Apr 03 2014

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