Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-SOX2 antibody [9-9-3]

Good
Abreviews
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Mouse Tissue sections (embryonic brain)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: 0.01M Sodium-citrate buffer, pH 6.0
Permeabilization
Yes - 0.3% Triton X-100 in PBS for 10 minutes
Specification
embryonic brain
Blocking step
10% goat serum + 1% BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 21°C
Fixative
Paraformaldehyde

Other product details

Incubation time
16 hour(s) and 0 minute(s) · Temperature: 21°C · Diluent: 1% BSA in PBS + 0.1%Triton X-100 in PBS
Dilution
1/500

Secondary antibody

Dilution
1/400
Name
Non-Abcam antibody was used: A-21422 (Life Technologies)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 555

Dr. Laura Lahti

Verified customer

Submitted Apr 20 2015

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