For the best experience on the Abcam website please upgrade to a modern browser such as Google Chrome
Xenopus laevis Recombinant protein (Clone expressed in Promega's TnT® SP6 High-Yield)
Gel Running Conditions
Non-reduced Denaturing (BioRad TGX Fast Cast 12%)
Clone expressed in Promega's TnT® SP6 High-Yield
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C
Other product details
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS-Tween
Non-Abcam antibody was used: donkey anti-mouse IgG-HRP
Host species: Donkey
Conjugation: Horse Radish Peroxidase
No-template IVT mix (lane 1), and sox21-FLAG IVT (lane 3).
anti-HA-HRP blot (blot C.)
Loading amount set to default 10ug. However, given these antibodies were tested qualitatively, the samples were not quantified.
Abcam user community
Submitted Jun 08 2018