Western blot abreview for Anti-SOX2 antibody [9-9-3]

Excellent
Abreviews
Application
Western blot
Sample
Xenopus laevis Recombinant protein (Clone expressed in Promega's TnT® SP6 High-Yield)
Gel Running Conditions
Non-reduced Denaturing (BioRad TGX Fast Cast 12%)
Loading amount
10 µg
Specification
Clone expressed in Promega's TnT® SP6 High-Yield
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% Milk in PBS-Tween
Dilution
1/1000

Secondary antibody

Dilution
1/3000
Name
Non-Abcam antibody was used: donkey anti-mouse IgG-HRP
Host species: Donkey
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
No-template IVT mix (lane 1), and sox21-FLAG IVT (lane 3).
Exposure
30 second(s)
Bands
Specific: 37 kDa
Positive control
anti-HA-HRP blot (blot C.)

Additional data

Additional Notes
Loading amount set to default 10ug. However, given these antibodies were tested qualitatively, the samples were not quantified.

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Submitted Jun 08 2018

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